المؤلفون: LANDRIEL, SOLEDAD CAMINATA, CASTILLO, JULIETA D.L.M., TABOGA, OSCAR A., FERRAROTTI, SUSANA A., GOTTLIEB, ALEXANDRA M., COSTA, HERNÁN

المصدر: Anais da Academia Brasileira de Ciências. January 2019 91(3)

مصطلحات موضوعية: Cyclodextrin glycosyltransferases, housekeeping genes, Paenibacillus, 16S rRNA

الوصف: Cyclodextrin glycosyltransferases (CGTases) are important enzymes in the biotechnology field because they catalyze starch conversion into cyclodextrins and linear oligosaccharides, which are used in food, pharmaceutical and cosmetic industries. The CGTases are classified according to their product specificity in α-, β-, α/β- and γ-CGTases. As molecular markers are the preferred tool for bacterial identification, we employed six molecular markers (16S rRNA, dnaK, gyrB, recA, rpoB and tufA) to test the identification of a CGTase-producing bacterial strain (DF 9R) in a phylogenetic context. In addition, we assessed the phylogenetic relationship of CGTases along bacterial evolution. The results obtained here allowed us to identify the strain DF 9R as Paenibacillus barengoltzii, and to unveil a complex origin for CGTase types during archaeal and bacterial evolution. We postulate that the α-CGTase activity represents the ancestral type, and that the γ-activity may have derived from β-CGTases.

وصف الملف: text/html

الوصول الحر: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500620Test

المؤلفون: Masip, Yamil E., Caeiro, Lucas D., Cosenza, Maximiliano, Postan, Miriam, Molina, Guido, Taboga, Oscar, Molinari, María Paula, Tekiel, Valeria

المساهمون: Agencia Nacional de Promoción Científica y Tecnológica

المصدر: Frontiers in Cellular and Infection Microbiology ; volume 14 ; ISSN 2235-2988

مصطلحات موضوعية: Infectious Diseases, Microbiology (medical), Immunology, Microbiology

الوصف: Chagas’ is a neglected disease caused by the eukaryotic kinetoplastid parasite, Trypanosoma cruzi. Currently, approximately 8 million people are infected worldwide, most of whom are in the chronic phase of the disease, which involves cardiac, digestive, or neurologic manifestations. There is an urgent need for a vaccine because treatments are only effective in the initial phase of infection, which is generally underdiagnosed. The selection and combination of antigens, adjuvants, and delivery platforms for vaccine formulations should be designed to trigger mixed humoral and cellular immune responses, considering that T. cruzi has a complex life cycle with both intracellular and bloodstream circulating parasite stages in vertebrate hosts. Here, we report the effectiveness of vaccination with a T. cruzi -specific protein family (TcTASV), employing both recombinant proteins with aluminum hydroxide and a recombinant baculovirus displaying a TcTASV antigen at the capsid. Vaccination stimulated immunological responses by producing lytic antibodies and antigen-specific CD4+ and CD8+ IFNɣ secreting lymphocytes. More than 90% of vaccinated animals survived after lethal challenges with T. cruzi , whereas all control mice died before 30 days post-infection. Vaccination also induced a strong decrease in chronic tissue parasitism and generated immunological memory that allowed vaccinated and infected animals to control both the reactivation of the infection after immunosuppression and a second challenge with T. cruzi . Interestingly, inoculation with wild-type baculovirus partially protected the mice against T. cruzi . In brief, we demonstrated for the first time that the combination of the baculovirus platform and the TcTASV family provides effective protection against Trypanosoma cruz i, which is a promising vaccine for Chagas disease.

الإتاحة: https://doi.org/10.3389/fcimb.2024.1297321Test

المؤلفون: López, María Gabriela, López, Cinthia Ayelén, Gravisaco, María José, Alfonso, Victoria, Taboga, Oscar

المصدر: Archives of Virology; May2024, Vol. 169 Issue 5, p1-11, 11p

مستخلص: The occlusion bodies of Autographa californica multiple nucleopolyhedrovirus are proteinaceous formations with significant biotechnological potential owing to their capacity to integrate foreign proteins through fusion with polyhedrin, their primary component. However, the strategy for successful heterologous protein inclusion still requires further refinement. In this study, we conducted a comparative assessment of various conditions to achieve the embedding of recombinant proteins within polyhedra. Two baculoviruses were constructed: AcPHGFP (polh+), with GFP as a fusion to wild type (wt) polyhedrin and AcΔPHGFP (polh+), with GFP fused to a fragment corresponding to amino acids 19 to 110 of polyhedrin. These baculoviruses were evaluated by infecting Sf9 cells and stably transformed Sf9, Sf9POLH, and Sf9POLHE44G cells. The stably transformed cells contributed another copy of wt or a mutant polyhedrin, respectively. Polyhedra of each type were isolated and characterized by classical methods. The fusion PHGFP showed more-efficient incorporation into polyhedra than ΔPHGFP in the three cell lines assayed. However, ΔPHGFP polyhedron yields were higher than those of PHGFP in Sf9 and Sf9POLH cells. Based on an integral analysis of the studied parameters, it can be concluded that, except for the AcΔPHGFP/Sf9POLHE44G combination, deficiencies in one factor can be offset by improved performance by another. The combinations AcPHGFP/Sf9POLHE44G and AcΔPHGFP/Sf9POLH stand out due to their high level of incorporation and the large number of recombinant polyhedra produced, respectively. Consequently, the choice between these approaches becomes dependent on the intended application. [ABSTRACT FROM AUTHOR]

: Copyright of Archives of Virology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Villafañe, Luciana, Rocha, Rosana Valeria, Bigi, María Mercedes, Klepp, Laura Inés, Taboga, Oscar Alberto, Forrellad, Marina Andrea, López, María Gabriela, Bigi, Fabiana

المصدر: Vet Immunol Immunopathol ; ISSN:1873-2534 ; Volume:273

مصطلحات موضوعية: Antigens, EsxG, IFN-γ assay, Mb0309, Mycobacterium bovis

الوصف: Bovine tuberculosis (bTB) represents a threat to livestock production. Mycobacterium bovis is the main causative agent of bTB and a pathogen capable of infecting wildlife and humans. Eradication programs based on surveillance in slaughterhouses with mandatory testing and culling of reactive cattle have failed to eradicate bTB in many regions worldwide. Therefore, developing effective tools to control this disease is crucial. Using a computational tool, we identified proteins in the M. bovis proteome that carry predictive binding peptides to BoLADRB3.2 and selected Mb0309, Mb1090, Mb1810 and Mb3810 from all the identified proteins. The expression of these proteins in a baculovirus-insect cell expression system was successful only for Mb0309 and Mb3810. In parallel, we expressed the ESAT-6 family proteins EsxG and EsxH in this system. Among the recombinant proteins, Mb0309 and EsxG exhibited moderate performance in distinguishing between cattle that test positive and negative to bTB using the official test, the intradermal tuberculin test (IDT), when used to stimulate interferon-gamma production in blood samples from cattle. However, when combined as a protein cocktail, Mb0309 and EsxG were reactive in 50 % of positive cattle. Further assessments in cattle that evade the IDT (false negative) and cattle infected with Mycobacterium avium paratuberculosis are necessary to determine the potential utility of this cocktail as an additional tool to assist the accurate diagnosis of bTB.

العلاقة: https://doi.org/10.1016/j.vetimm.2024.110788Test; https://pubmed.ncbi.nlm.nih.gov/38838485Test

الإتاحة: https://doi.org/10.1016/j.vetimm.2024.110788Test
https://pubmed.ncbi.nlm.nih.gov/38838485Test

المؤلفون: Plastine, María del Pilar, Amalfi, Sabrina, López, María Gabriela, Gravisaco, María José, Taboga, Oscar, Alfonso, Victoria

المصدر: Gene Therapy ; ISSN 0969-7128 1476-5462

مصطلحات موضوعية: Genetics, Molecular Biology, Molecular Medicine

الإتاحة: https://doi.org/10.1038/s41434-024-00442-4Test
https://www.nature.com/articles/s41434-024-00442-4.pdfTest
https://www.nature.com/articles/s41434-024-00442-4Test

المؤلفون: Cacciabue, Marco, Aguilera, Pablo, Gismondi, María Inés, Taboga, Oscar

المصدر: Infection, Genetics and Evolution ; volume 99, page 105261 ; ISSN 1567-1348

مصطلحات موضوعية: Infectious Diseases, Microbiology (medical), Genetics, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Microbiology

الإتاحة: https://doi.org/10.1016/j.meegid.2022.105261Test
https://api.elsevier.com/content/article/PII:S1567134822000582?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1567134822000582?httpAccept=text/plainTest

المؤلفون: Zanetti, Flavia A., Fernández, Ignacio, Baquero, Eduard, Guardado-Calvo, Pablo, Dubois, Sarah, Morel, Etienne, Alfonso, Victoria, Aguilera, Milton O., Celayes, María E., Polo, Luis M., Suhaiman, Laila, Galassi, Vanesa V., Chiarpotti, María V., Allende, Carolina, Rodríguez, Javier M., Castón, José R., Lijavetzky, Diego, Taboga, Oscar, Colombo, María I., Pópolo, Mario G. Del, Rey, Félix A., Delgui, Laura R.

الوصف: Birnaviruses form a distinct class of double-stranded RNA (dsRNA) viruses characterized by the absence of a transcription-competent inner core particle. The early endosomes (EE) of cells infected with the infectious bursal disease virus (IBDV) - a prototypical birnavirus and an important avian pathogen - constitute a platform for viral replication. Here, we study the mechanism of birnaviral hijacking of EE membranes for this process. We demonstrate that the viral protein 3 (VP3) specifically binds to phosphatidylinositol-3-phosphate (PI3P) present in EE membranes. We identify the domain of VP3 involved in PI3P-binding and its role in viral replication. Finally, our molecular simulations results unveil a two-stage modular mechanism for VP3 association with EE. Firstly, the carboxy-terminal region of VP3 adsorbs to the membrane via non-specific electrostatic interactions. Then, in the second stage, the VP3 core seals the membrane engagement by specifically binding PI3P through its P2 domain, additionally promoting PI3P accumulation.

الإتاحة: https://doi.org/10.7554/elife.97261Test
https://elifesciences.org/reviewed-preprints/97261v1/pdfTest

المؤلفون: Zanetti, Flavia A., Fernández, Ignacio, Baquero, Eduard, Guardado-Calvo, Pablo, Dubois, Sarah, Morel, Etienne, Alfonso, Victoria, Aguilera, Milton O., Celayes, María E., Polo, Luis M., Suhaiman, Laila, Galassi, Vanesa V., Chiarpotti, María V., Allende, Carolina, Rodríguez, Javier M., Castón, José R., Lijavetzky, Diego, Taboga, Oscar, Colombo, María I., Pópolo, Mario G. Del, Rey, Félix A., Delgui, Laura R.

الإتاحة: https://doi.org/10.7554/elife.97261.1.sa4Test

المؤلفون: Molina, Guido Nicolas, Amalfi, Sabrina, Otero, Ignacio, Taboga, Oscar Alberto, Molinari, Maria Paula

المصدر: Veterinary Sciences 8 (11) : 278 (Noviembre 2021)

مصطلحات موضوعية: Baculovirus, Interferons, Swine, Antiviral Agents, Interferonas, Cerdo, Autographa californica, Viricidas

الوصف: The huge variety of viruses affecting swine represents a global threat. Since vaccines against highly contagious viruses last several days to induce protective immune responses, antiviral strategies for rapid control of outbreak situations are needed. The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), an insect virus, has been demonstrated to be an effective vaccine vector for mammals. Besides the ability to display or transduce heterologous antigens, it also induces strong innate immune responses and provides IFN-mediated protection against lethal challenges with viruses like foot-and-mouth disease virus (FMDV) in mice. Thus, the aim of this study was to evaluate the ability of AcMNPV to induce IFN production and elicit antiviral activity in porcine peripheral blood mononuclear cells (PBMCs). Our results demonstrated that AcMNPV induced an IFN-α-mediated antiviral activity in PBMCs in vitro. Moreover, the inoculation of AcMNPV in piglets led to the production of type I and II IFNs in sera from inoculated animals and antiviral activities against vesicular stomatitis virus (VSV) and FMDV measured by in vitro assays. Finally, it was demonstrated that the pseudotyping of AcMNPV with VSV-G protein, but not the enrichment of the AcMNPV genome with specific immunostimulatory CpG motifs for the porcine TLR9, improved the ability to induce IFN-α production in PBMCs in vitro. Together, these results suggest that AcMNPV is a promising tool for the induction of IFNs in antiviral strategies, with the potential to be biotechnologically improved. ; Instituto de Biotecnología ; Fil: Molina, Guido Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina ; Fil: Molina, Guido Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina ; Fil: Amalfi, Sabrina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina ; Fil: Amalfi, Sabrina. ...

وصف الملف: application/pdf

العلاقة: info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud?; http://hdl.handle.net/20.500.12123/16611Test; https://www.mdpi.com/2306-7381/8/11/278Test; https://doi.org/10.3390/vetsci8110278Test

الإتاحة: https://doi.org/20.500.12123/16611Test
https://doi.org/10.3390/vetsci8110278Test
https://hdl.handle.net/20.500.12123/16611Test
https://www.mdpi.com/2306-7381/8/11/278Test

المؤلفون: Smith, Ignacio, Mc Callum, Gregorio Juan, Sabljic, Adriana Victoria, Marfia, Juan Ignacio, Bombicino, Silvina Sonia, Trabucchi, Aldana, Iacono, Ruben Francisco, Birenbaum, Joaquín Manuel, Vazquez, Susana Claudia, Minoia, Juan Mauricio, Cascone, Osvaldo, López, María Gabriela, Taboga, Oscar, Targovnik, Alexandra Marisa, Wolman, Federico Javier, Fingermann, Matías, Alonso, Leonardo Gabriel, Valdez, Silvina Noemí, Miranda, María Victoria

المساهمون: Fondo para la Investigación Científica y Tecnológica

المصدر: Biotechnology and Bioengineering ; volume 118, issue 10, page 4129-4137 ; ISSN 0006-3592 1097-0290

الوصف: Serology testing for COVID‐19 is important in evaluating active immune response against SARS‐CoV‐2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, the duration of antibodies in virus‐exposed individuals and vaccine‐mediated immunity. In this study, recombinant S protein of SARS‐CoV‐2 was expressed in Rachiplusia nu , an important agronomic plague. One gram of insect larvae produces an amount of S protein sufficient for 150 determinations in the ELISA method herein developed. We established a rapid production process for SARS‐CoV‐2 S protein that showed immunoreactivity for anti‐SARS‐CoV‐2 antibodies and was used as a single antigen for developing the ELISA method with high sensitivity (96.2%) and specificity (98.8%). Our findings provide an efficient and cost‐effective platform for large‐scale S protein production, and the scale‐up is linear, thus avoiding the use of complex equipment like bioreactors.

الإتاحة: https://doi.org/10.1002/bit.27889Test