المؤلفون: O’Connor, Aoife M., Seunik, Maximillian, Radi, Blas, Matthyse, Liberty, Gable, Lance, Huffstetler, Hanna E., Meier, Benjamin Mason

المصدر: Journal of Law, Medicine & Ethics ; volume 50, issue 3, page 409-424 ; ISSN 1073-1105 1748-720X

الوصف: Despite a recent wave in global recognition of the rights of transgender and gender-diverse populations, referred to in this text by the umbrella label of trans*, international law continues to presume a cisgender binary definition of gender — dismissing the lived realities of trans* individuals throughout the world. This gap in international legal recognition and protection has fundamental implications for health, where trans* persons have been and continue to be subjected to widespread discrimination in health care, longstanding neglect of health needs, and significant violations of bodily autonomy.

الإتاحة: https://doi.org/10.1017/jme.2022.84Test
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1073110522000845Test

المؤلفون: Hibbitts, Alan, O’Connor, Aoife M., McCarthy, Joanna, Forde, Éanna B., Hessman, Gary, O’Driscoll, Caitriona M., Cryan, Sally-Ann, Devocelle, Marc

المساهمون: Science Foundation Ireland, Higher Education Authority

المصدر: ACS Omega ; volume 4, issue 6, page 10078-10088 ; ISSN 2470-1343 2470-1343

الإتاحة: https://doi.org/10.1021/acsomega.9b00265Test

المؤلفون: O’Connor, Aoife M., McManus, Brenda A., Kinnevey, Peter M., Brennan, Gráinne I., Fleming, Tanya E., Cashin, Phillipa J., O’Sullivan, Michael, Polyzois, Ioannis, Coleman, David C.

المصدر: Frontiers in Microbiology ; volume 9 ; ISSN 1664-302X

الإتاحة: https://doi.org/10.3389/fmicb.2018.01558Test

المؤلفون: O'Connor, Aoife M., McManus, Brenda A., Coleman, David C.

المساهمون: Microbiology Research Unit, Dublin Dental University Hospital

المصدر: Infection, Genetics and Evolution ; volume 61, page 60-66 ; ISSN 1567-1348

مصطلحات موضوعية: Infectious Diseases, Microbiology (medical), Genetics, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Microbiology

الإتاحة: https://doi.org/10.1016/j.meegid.2018.03.012Test
https://api.elsevier.com/content/article/PII:S1567134818301114?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S1567134818301114?httpAccept=text/plainTest

المؤلفون: McManus, Brenda A., O'Connor, Aoife M., Kinnevey, Peter M., O'Sullivan, Michael, Polyzois, Ioannis, Coleman, David C.

المصدر: Antimicrobial Agents and Chemotherapy ; volume 61, issue 10 ; ISSN 0066-4804 1098-6596

الوصف: The type III arginine catabolic mobile element (ACME) was detected in three Staphylococcus epidermidis oral isolates recovered from separate patients (one healthy, one healthy with dental implants, and one with periodontal disease) based on ACME- arc -operon- and ACME- opp3 -operon-directed PCR. These isolates were subjected to whole-genome sequencing to characterize the precise structural organization of ACME III for the first time, which also revealed that all three isolates were the same sequence type, ST329.

الإتاحة: https://doi.org/10.1128/aac.01216-17Test

المؤلفون: O'Connor, Aoife M.¹, Seunik, Maximillian¹, Radi, Blas², Matthyse, Liberty³, Gable, Lance⁴, Huffstetler, Hanna E.⁵, Meier, Benjamin Mason⁵

المصدر: Journal of Law, Medicine & Ethics. Fall2022, Vol. 50 Issue 3, p409-424. 16p.

مصطلحات موضوعية: *HEALTH services accessibility, *HUMAN rights, *LEGAL status of transgender people, *BINARY gender system, *DISCRIMINATION (Sociology), *AUTONOMY (Psychology), *MEDICAL needs assessment

مستخلص: Despite a recent wave in global recognition of the rights of transgender and gender-diverse populations, referred to in this text by the umbrella label of trans*, international law continues to presume a cisgender binary definition of gender — dismissing the lived realities of trans* individuals throughout the world. This gap in international legal recognition and protection has fundamental implications for health, where trans* persons have been and continue to be subjected to widespread discrimination in health care, longstanding neglect of health needs, and significant violations of bodily autonomy. [ABSTRACT FROM AUTHOR]

المؤلفون: O’Connor, Aoife M., McManus, Brenda A., Kinnevey, Peter M., Brennan, Gráinne I., Fleming, Tanya E., Cashin, Phillipa J., O’Sullivan, Michael, Polyzois, Ioannis, Coleman, David C.

المصدر: Frontiers in Microbiology; 7/12/2018, pN.PAG-N.PAG, 15p

مصطلحات موضوعية: ARGININE, MOBILE genetic elements, STAPHYLOCOCCUS epidermidis

مستخلص: Staphylococcus aureus and Staphylococcus epidermidis are frequent commensals of the nares and skin and are considered transient oral residents. Reports on their prevalence in the oral cavity, periodontal pockets and subgingivally around infected oral implants are conflicting, largely due to methodological limitations. The prevalence of these species in the oral cavities, periodontal pockets and subgingival sites of orally healthy individuals with/without implants and in patients with periodontal disease or infected implants (peri-implantitis) was investigated using selective chromogenic agar and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Staphylococcus epidermidis was predominant in all participant groups investigated. Its prevalence was significantly higher (P = 0.0189) in periodontal pockets (30%) than subgingival sites of healthy individuals (7.8%), and in subgingival peri-implantitis sites (51.7%) versus subgingival sites around non-infected implants (16.1%) (P = 0.0057). In contrast, S. aureus was recovered from subgingival sites of 0-12.9% of the participant groups, but not from periodontal pockets. The arginine catabolic mobile element (ACME), thought to enhance colonization and survival of S. aureus, was detected in 100/179 S. epidermidis and 0/83 S. aureus isolates screened using multiplex PCR and DNA microarray profiling. Five distinct ACME types, including the recently described types IV and V (I; 14, II; 60, III; 10, IV; 15, V; 1) were identified. ACME-positive S. epidermidis were significantly (P = 0.0369) more prevalent in subgingival peri-implantitis sites (37.9%) than subgingival sites around non-infected implants (12.9%) and also in periodontal pockets (25%) compared to subgingival sites of healthy individuals (4.7%) (P = 0.0167). To investigate the genetic diversity of ACME, 35 isolates, representative of patient groups, sample sites and ACME types underwent whole genome sequencing from which multilocus sequence types (STs) were identified. Sequencing data permitted ACME types II and IV to be subdivided into subtypes IIa-c and IVa-b, respectively, based on distinct flanking direct repeat sequences. Distinct ACME types were commonly associated with specific STs, rather than health/disease states or recovery sites, suggesting that ACME types/subtypes originated amongst specific S. epidermidis lineages. Ninety of the ACME-positive isolates encoded the ACME-arc operon, which likely contributes to oral S. epidermidis survival in the nutrient poor, semi-anaerobic, acidic and inflammatory conditions present in periodontal disease and peri-implantitis. [ABSTRACT FROM AUTHOR]

: Copyright of Frontiers in Microbiology is the property of Frontiers Media S.A. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: McManus, Brenda A.¹ (AUTHOR), O'Connor, Aoife M.¹ (AUTHOR), Egan, Sarah A.¹ (AUTHOR), Flanagan, Peter R.¹ (AUTHOR), Coleman, David C.¹ (AUTHOR) david.coleman@dental.tcd.ie

المصدر: Infection, Genetics & Evolution. Jul2019, Vol. 71, p51-53. 3p.

مصطلحات موضوعية: *STAPHYLOCOCCUS, *STAPHYLOCOCCUS epidermidis, *NUCLEOTIDE sequencing, *METHICILLIN-resistant staphylococcus aureus, *DENTAL plaque, *OPERONS

مستخلص: The arginine catabolic mobile element (ACME) was first described in methicillin-resistant Staphylococcus aureus and is considered to enhance transmission, persistence and survival. Subsequently ACMEs were shown to be more prevalent in the coagulase-negative Staphylococcus epidermidis. Previously, ACME types were distinguished by characteristic combinations of the arc and opp3 operons [I (arc +, opp3 +), II (arc +, opp3 -) and III (arc -, opp3 +)] encoding an arginine deaminase pathway and oligopeptide permease transporter, respectively. Recently two novel ACME types harboring the potassium transporter-encoding operon kdp were described in oral S. epidermidis isolates [IV (arc +, opp3 -, kdp +), and V (arc +, opp3 +, kdp +)]. This study investigated two independent oral S. epidermidis isolates that yielded amplimers with kdp -directed primers only when subjected to ACME typing PCRs. Hybrid assemblies based on Illumina MiSeq short-read and Oxford Nanopore MinION long-read whole genome sequences revealed that both isolates harbored a sixth, novel ACME type (VI) integrated into orfX. Both ACME VIs lacked the arc and opp3 operons, harbored the kdp operon adjacent to other commonly ACME-associated genes including speG , hsd , sdr , and rep, but the structural organization of the adjacent regions were distinct. These ACMEs were flanked by different direct repeat sequences and the ACME VI-positive isolates belonged to unrelated genetic clusters. Overall these findings are indicative of independent evolution. The identification of ACME type VI further illustrates the diversity of ACME elements in S. epidermidis. The presence of ACMEs harboring kdp may confer a selective advantage on oral S. epidermidis in a potassium-rich environment such as found in dental plaque. Image 1 • First description of novel arginine catabolic mobile element (ACME) type VI • The ACME type VI element harbors the kdp operon but lacks the arc and opp3 operons. • Two phylogenetically distinct oral S. epidermidis isolates harbored ACME VI. • Each ACME VI investigated was flanked by distinct direct repeat sequences. • The kdp operon encodes a potassium transporter system. [ABSTRACT FROM AUTHOR]

المؤلفون: McManus, Brenda A., O'Connor, Aoife M., Kinnevey, Peter M., O'Sullivan, Michael, Polyzois, Ioannis, Coleman, David C.

المصدر: Antimicrobial Agents and Chemotherapy; August 2017, Vol. 61 Issue: 10

مستخلص: ABSTRACTThe type III arginine catabolic mobile element (ACME) was detected in three Staphylococcus epidermidisoral isolates recovered from separate patients (one healthy, one healthy with dental implants, and one with periodontal disease) based on ACME-arc-operon- and ACME-opp3-operon-directed PCR. These isolates were subjected to whole-genome sequencing to characterize the precise structural organization of ACME III for the first time, which also revealed that all three isolates were the same sequence type, ST329.