التفاصيل البيبلوغرافية
| العنوان: |
Slow Folding–Unfolding Kinetics of an Octameric β‑Peptide Bundle |
| المؤلفون: |
Montalvo, Geronda L, Gai, Feng, Roder, Heinrich, DeGrado, William F |
| المصدر: |
ACS Chemical Biology, vol 9, iss 1 |
| بيانات النشر: |
eScholarship, University of California |
| سنة النشر: |
2014 |
| المجموعة: |
University of California: eScholarship |
| مصطلحات موضوعية: |
Kinetics, Oligopeptides, Protein Folding, Protein Structure, Secondary, Temperature, Thermodynamics, Tyrosine, Chemical Sciences, Biological Sciences, Organic Chemistry |
| جغرافية الموضوع: |
276 - 281 |
| الوصف: |
β-Peptide foldamers offer attractive frameworks for examining the effect of backbone flexibility on the dynamics of protein folding. Herein, we study the folding-unfolding kinetics of a β-peptide, Acid-1Y,1 which folds in aqueous solution into an octameric bundle of peptides in a conformation known as the 14-helix. Acid-1Y is comprised exclusively of β-amino acids, which differ from α-amino acids by the addition of a single methylene into the backbone. We aim to understand how the additional degree of freedom and increased backbone flexibility in the β-amino acid affect folding dynamics and to measure folding rates of this octameric β-peptide. Previously, we found that the T-jump induced relaxation kinetics of a monomeric β-peptide that forms a monomeric 14-helix occurred on the nanosecond time scale2 and were noticeably slower than a similar alanine-based α-helical peptide.3 Additionally, in comparison to similar α-helices, the relaxation rates showed a weaker dependence on temperature. Here, we find that the T-jump induced relaxation kinetics of the octameric β-peptide occurs on an even slower time scale (minutes) and the unfolding relaxation rates show a large dependence on temperature. These differences indicate that folding energy landscapes of β-peptide secondary and quaternary structure are markedly distinct from one another and also from their α-helical counterparts. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| العلاقة: |
qt27z2x74f; https://escholarship.org/uc/item/27z2x74fTest |
| الإتاحة: |
https://escholarship.org/uc/item/27z2x74fTest |
| حقوق: |
public |
| رقم الانضمام: |
edsbas.69D62082 |
| قاعدة البيانات: |
BASE |
