المؤلفون: Shiohara, Tetsuo¹, Moriya, Noriko¹, Gotoh, Chie¹, Gomi, Toshihiko¹, Nigashima, Masaji¹

المصدر: Journal of Investigative Dermatology. Mar1989, Vol. 92 Issue 3, p360-365. 6p.

مصطلحات موضوعية: *ANTIGENS, *EPIDERMIS, *CELLS, *BIOLOGICAL assay, *LYMPH nodes, *KERATINOCYTES

مستخلص: Three types of L3T4+ cloned T cells with different antigen specificities, auto-, allo-, and antigen-reactive, were characterized with respect to their migratory potential using an in vitro migration assay under agar gel. Autoreactive T cells, BB5, and alloreactive T cells, SK 1, both of which have been proved to be epidermotropic in vivo, showed specific directional migration to the epidermis, whereas no directional migration was seen with non-epidermotropic cloned T cells and freshly isolated lymph node T cells. Both BB5 and SK 1 cells were equally attracted to all the epidermal fragments tested regardless of their I-A antigens. The directional migration of BB5 cells to the epidermis was significantly inhibited by the co-cultivation with the epidermis, but not the dermis. Studies with cell lines, the conditioned media (CM), and recombinant interleukin (IL) 1, 2, and 3 revealed that BB5 cells were chemotactically attracted to a transformed keratinocyte cell line PAM212 and, to a lesser extent, to the CM from PAM212 and IL-2, but not to IL-1 and IL 3. These results suggest that epidermotropic T cells may be preferentially trapped in an area with a high concentration of keratinocyte-derived growth factors as well as IL-2. [ABSTRACT FROM AUTHOR]

المؤلفون: Shiohara, Tetsuo¹, Moriya, Noriko¹, Gotoh, Chie¹, Saizawa, Kaj², Nagashima, Masaji¹

المصدر: Journal of Investigative Dermatology. Jul88, Vol. 91 Issue 1, p69-75. 7p.

مصطلحات موضوعية: *TISSUES, *T cells, *KERATINOCYTES, *SERUM, *EPIDERMIS, *CELLS

مستخلص: The role of Ia+ keratinocytes in epidermotropism of T cells was analyzed by suing self-IA specific autoreactive cloned T cells with epidermotropic nature (termed BB5) and those without it (termed C10). These T-cell clones were injection of normal mouse serum. Ia expression by keratinocytes was associated with the increased epidermal invasion of BB5 cells, but did nor render C10 cells capable of migrating into the epidermis. The migration of the T cells to epidermis was also studied in vitro using a migration assay under agar. Ia expression by keratinocytes significantly enhanced the in vitro migration of BB5 cells to the epidermis, but had no effect on the migration of C10 cells and freshly isolated unstimulated lymphocyts. We surmise from these results that Ia+keratinocytes may facilitate the epidermotorpic nature, but not those without it. However, the possibility certainly exists that the observed preferential migration of BB5 cells to Ia+ keratinocyts may be secondary to the alteration of other factors associated with the Ia expression. The injection of normal mouse serum was accompanied by an increase in the production of epidermal cell-derived thymocyte activating factor (ETAF) indicating that the increased ETAF production may have contributed to some of the observed preferential migration of BB5 cells. [ABSTRACT FROM AUTHOR]

المؤلفون: Shiohara, Tetsuo¹, Moriya, Noriko¹, Mochizuki, Tomoko¹, Nagashima, Masaji¹

المصدر: Journal of Investigative Dermatology. Jul87, Vol. 89 Issue 1, p8-14. 7p. 15 Black and White Photographs, 1 Chart, 1 Graph.

مصطلحات موضوعية: *T cells, *LYMPHOCYTES, *PHENOTYPES, *LYMPHOKINES, *KERATINOCYTES, *LEUCOCYTES, *INTERFERONS, *LANGERHANS cells, *EPIDERMIS

مستخلص: Recent studies have suggested that T lymphocytes with helper phenotype may play an important role in the pathogenesis of lichenoid tissue reactions (LTR). In order to elucidate whether murine self-Ia-specific autoreactive T cells with helper phenotype can induce LTR in naive syngeneic hosts, two clones of autoreactive cloned T cells with both helper and cytotoxic activities, capable of producing the cytotoxic lymphokines gamma interferon and lymphotoxin in response to self-la antigens, were examined for their ability to induce LTR. One clone, BB5, when injected into footpads of syngeneic hosts, induced prominent epidermotropic cellular infiltrates that led to basal vacuolar degeneration and Civatte body formation; the other clone, CIO, did not. No difference between BB5 and CIO was detected with respect to their ability to induce delayed-type hypersensitivity reactions. Immunohistochemical studies using anti-Lyt monoclonal antibodies showed that BBS-induced LTR was induced by the epidermal invasion of the injected BB5 cells themselves. We further observed that the infusion of BB5 cells not only resulted in la antigen expression on keratinocytes, but was also accompanied by a morphologic change in Langerhans cells at 24 h, when the epidermal invasion had not yet developed. However, similar observations were also observed with CIO cells, indicating that la expression on keratinocytes may not be a prerequisite for the induction of LTR. [ABSTRACT FROM AUTHOR]

المؤلفون: Kano, Yoko, Chiba, Masako, Yagita, Akikuni, Shiohara, Tetsuo

المصدر: International Journal of Dermatology; Apr1997, Vol. 36 Issue 4, p280-282, 3p

مصطلحات موضوعية: PSORIASIS, SURGICAL excision, SKIN cancer, CYTOKINES, CANCER cells, KERATINOCYTES

مستخلص: The article focuses on clinical course of psoriasis after excision of an underlying malignancy, and complete remission of psoriasis on excision of an underlying carcinoma. There are several possible explanations why psoriatic lesions resolve completely after the excision of the thyroid carcinoma. One possibility is that the treatment modalities used were incidentally responsible for the resolution of the patient's psoriasis. A second possibility is that tumor cells may produce growth factors and cytokines with potent stimulatory effects on epidermal keratinocytes.