Identification of pathogens by comprehensive real-time PCR versus conventional methods in community-acquired pneumonia in Japanese adults
| العنوان: | Identification of pathogens by comprehensive real-time PCR versus conventional methods in community-acquired pneumonia in Japanese adults |
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| المؤلفون: | Kenichiro Shimizu, Katsutoshi Nakayama, Naoki Takasaka, Yutaka Yoshii, Shunsuke Minagawa, Saburo Ito, Shigeo Hanada, Hiromichi Hara, Kazuma Kishi, Yumi Kaneko, Keisuke Saito, Miyuki Morozumi, Takanori Numata, Hironori Uruga, Jun Kojima, Jun Araya, Makoto Kawaishi, Kazuyoshi Kuwano, Naoko Chiba, Kimiko Ubukata, Hiroshi Wakui |
| المصدر: | Infectious Diseases. 48:782-788 |
| بيانات النشر: | Informa UK Limited, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Adult, Male, Microbiological Techniques, 0301 basic medicine, Microbiology (medical), medicine.medical_specialty, 030106 microbiology, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Microbiology, law.invention, 03 medical and health sciences, High morbidity, Japan, Community-acquired pneumonia, law, Nasopharynx, Internal medicine, medicine, Humans, Prospective Studies, Antibiotic use, Polymerase chain reaction, Aged, Aged, 80 and over, Bacteria, General Immunology and Microbiology, business.industry, Sputum, Pneumonia, General Medicine, Middle Aged, medicine.disease, Community-Acquired Infections, Infectious Diseases, Real-time polymerase chain reaction, Molecular Diagnostic Techniques, Viruses, Pneumococcal pneumonia, Female, medicine.symptom, business |
| الوصف: | Community-acquired pneumonia (CAP) has high morbidity and mortality. Unfortunately, the pathogen detection rate using conventional culture methods is relatively low. We compared comprehensive real-time polymerase chain reaction (real-time PCR) analysis of nasopharyngeal swab specimens (NPS) and sputum samples against conventional methods for ability to detect causative pathogens of CAP.We prospectively enrolled adult CAP patients, including those with prior antibiotic use, from December 2012 to May 2014. For each patient, causative pathogens were investigated conventionally and by real-time PCR that can identify 6 bacterial and 11 viral pathogens.Patients numbered 92 (mean age, 63 years; 59 male), including 30 (33%) with prior antibiotic use. Considering all patients, identification of causative pathogens by real-time PCR was significantly more frequent than by conventional methods in all patients (72% vs. 57%, p = 0.018). In patients with prior antibiotic use, identification rates also differed significantly (PCR, 77%; conventional, 50%; p = 0.027). Mixed infections were more frequent according to real-time PCR than conventional methods (26% vs. 4%, p 0.001). By the real-time PCR, Streptococcus pneumoniae was most frequently identified (38%) as a causative pathogen, followed by Haemophilus influenzae (37%) and Mycoplasma pneumoniae (5%). PCR also identified viral pathogens (21%), with sensitivity enhanced by simultaneous examination of both NPS and sputum samples rather than only NPS samples.Real-time PCR of NPS and sputum samples could better identify bacterial and viral pathogens in CAP than conventional methods, both overall and in patients with prior antibiotic treatment. |
| تدمد: | 2374-4243 2374-4235 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bcfa7098aa05accc91d6d9dfcd0de96Test https://doi.org/10.1080/23744235.2016.1193788Test |
| رقم الانضمام: | edsair.doi.dedup.....6bcfa7098aa05accc91d6d9dfcd0de96 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23744243 23744235 |
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