دورية أكاديمية
IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation
| العنوان: | IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation |
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| المؤلفون: | Daria Kartasheva-Ebertz, Jesintha Gaston, Loriane Lair-Mehiri, Estelle Mottez, Tan-Phuc Buivan, Pierre-Philippe Massault, Olivier Scatton, Sebastien Gaujoux, Jean-Christophe Vaillant, Stanislas Pol, Sylvie Lagaye |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 9773, p 9773 (2022) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2022 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | fibrosis, human liver, type 3 inflammation, IL-17A, human liver slice culture, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | IL-17A is considered to guide liver inflammation and fibrosis. From twenty-two human liver samples of different fibrosis stages (F0 to F4), IL-17A, IL-22, and TGFβ1 protein expression in liver tissue lysates were analyzed. Ten paired samples of liver tissue (F0–F1 stage) and blood from the same patient were used to analyze intrahepatic and blood T-lymphoid IL-17A + cells by flow cytometry. The analyses have been performed regardless of pathology, considering the stage of fibrosis. Human liver tissue was used for the primary human liver slice cultures, followed by subsequent cytokine stimulation and fibrotic markers’ analysis by ELISA. IL-17A production in human liver tissue was significantly higher in the early fibrotic stage compared with the advanced stage. Th17 T cells and, to a lesser extent, MAIT cells were the main sources of IL-17A in both compartments, the liver and the blood. Moreover, the presence of liver Th17IL-17A + INFγ + cells was detected in the liver. IL-17A stimulation of human liver slice culture increased the expression of profibrotic and pro-inflammatory markers. IL-17A, secreted by Th17 and MAIT cells in the liver, triggered fibrosis by inducing the expression of IL-6 and profibrotic markers and could be a target for antifibrotic treatment. Further amplitude studies are needed to confirm the current results. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| العلاقة: | https://www.mdpi.com/1422-0067/23/17/9773Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test; https://doaj.org/article/13e744ab665d4aafb2626c08cd3b787aTest |
| DOI: | 10.3390/ijms23179773 |
| الإتاحة: | https://doi.org/10.3390/ijms23179773Test https://doaj.org/article/13e744ab665d4aafb2626c08cd3b787aTest |
| رقم الانضمام: | edsbas.26690BAF |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms23179773 |