التفاصيل البيبلوغرافية
| العنوان: |
Histomonas meleagridis isolates compared by virulence and gene expression. |
| المؤلفون: |
Wei, Zehui1 (AUTHOR), Abraham, Mathew2 (AUTHOR), Chadwick, Elle V.3 (AUTHOR), Beckstead, Robert B.3 (AUTHOR) beckstead@ncsu.edu |
| المصدر: |
Veterinary Parasitology. Oct2020, Vol. 286, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*CYSTEINE proteinases, *GENE expression, *POLYMERASE chain reaction, *SUPEROXIDE dismutase, *WEIGHT gain, *MONOCULTURE agriculture |
| مصطلحات جغرافية: |
UNITED States |
| مستخلص: |
• H. meleagridis isolates do not differ in pathogenicity based on geographic origin in the United States. • Expression pattern of various cysteine proteinases differ between H. meleagridis isolates. • SOD may act as a protective mechanism for H. meleagridis in oxygen-rich environments. Pathology and putative virulence factor expression of three Histomonas meleagridis isolates differing in geographic origin, cell passage number (56 or 100), or cell populations grown from a monoculture were compared. Turkey poults inoculated with the high cell passage number isolates or monoculture isolates varied in gross lesion severity and weight gain (P<0.0001). Screening of a published H. meleagridis cDNA library identified forty- eight cysteine proteinases (CP) and one superoxide dismutase (Fe-SOD) proposed to function in either tissue damage and/or invasion and oxidative defense. The Fe-SOD and eight CPs were analyzed using real time polymerase chain reaction. CP2, CP3, and CP8 showed significant differences in expression among the field isolates (P ≤ 0.05). The high passage isolates had decreased CP2, CP3 and CP4 expression when compared with their field isolate. CP7 did not differ between field isolates or the 56-passaged isolate. The Fe-SOD gene showed significant differences in expression among the various isolates. When exposing cultured H. meleagridis to air, Fe-SOD expression decreased rapidly during the first hour of air exposure but increased progressively through the next 3 h. This study provides information on gross pathology and virulence factors associated with various isolates of Histomonas meleagridis which can aid in determining the pathogenetic mechanisms used by this organism. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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