التفاصيل البيبلوغرافية
| العنوان: |
Human leukocyte antigen class I gene mutations in cervical cancer. |
| المؤلفون: |
Koopman, Louise A., Van Der Slik, Arno R., Koopman, L A, van Der Slik, A R, Giphart, M J, Fleuren, G J |
| المصدر: |
JNCI: Journal of the National Cancer Institute; 10/06/99, Vol. 91 Issue 19, p1669-1677, 9p, 5 Diagrams, 3 Charts |
| مصطلحات موضوعية: |
HLA histocompatibility antigens, ANTIGENS, GENETIC mutation, CHROMOSOMES, DNA probes, GENES, POLYMERASE chain reaction, CERVIX uteri tumors, PHENOTYPES, CANCER cell culture, GENOTYPES |
| مستخلص: |
Background: Various mechanisms contribute to the loss of human leukocyte antigen (HLA) class I expression that is frequently observed in cancers. Although some single allele losses have been ascribed to mutations in HLA class I genes, direct evidence for this phenomenon in vivo is still lacking. Thus, we investigated whether HLA class I gene mutations could account for the loss of allele-specific expression in cervical carcinomas.Methods: We used polymerase chain reaction-based techniques, including sequencing, oligonucleotide hybridization, and microsatellite analysis, to identify HLA class I gene defects in two tumor-derived cell lines and to confirm the presence of these defects in the original tumors.Results: In one tumor, in exon 2 of the HLA-B15 gene, a four-nucleotide insertion resulted in a stop codon in exon 3. In the other tumor, in two duplicated copies of the HLA-A24 gene, single-point mutations resulted in stop codons in exons 2 and 5.Conclusions: To our knowledge, this is the first report of HLA class I gene mutations identified in primary tumors that lead to loss of allelic expression in tumor cells. Such tumor-specific mutations may permit the cell to escape HLA class I-restricted cytotoxic T-cell responses. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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