Involvement of Alveolar Epithelial Cell Necroptosis in Idiopathic Pulmonary Fibrosis Pathogenesis
| العنوان: | Involvement of Alveolar Epithelial Cell Necroptosis in Idiopathic Pulmonary Fibrosis Pathogenesis |
|---|---|
| المؤلفون: | Mi So Kim, Ae-Rin Baek, Ji Min Lee, Do Jin Kim, Shunsuke Minagawa, Masahiro Yoshida, Su Sie Chin, An Soo Jang, Choon-Sik Park, Sung Woo Park, June-Hyuk Lee, Jun Araya, Kazuyoshi Kuwano |
| المصدر: | American Journal of Respiratory Cell and Molecular Biology. 59:215-224 |
| بيانات النشر: | American Thoracic Society, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Pulmonary and Respiratory Medicine, Programmed cell death, Necroptosis, Clinical Biochemistry, Apoptosis, Kidney, Bleomycin, Pathogenesis, Mice, Necrosis, 03 medical and health sciences, chemistry.chemical_compound, Idiopathic pulmonary fibrosis, 0302 clinical medicine, Fibrosis, medicine, Animals, Humans, Molecular Biology, Lung, Caspase 3, business.industry, Graft Survival, Epithelial Cells, Cell Biology, respiratory system, medicine.disease, respiratory tract diseases, 030104 developmental biology, medicine.anatomical_structure, chemistry, Receptor-Interacting Protein Serine-Threonine Kinases, 030220 oncology & carcinogenesis, Cancer research, business |
| الوصف: | Alveolar epithelial cell (AEC) injury leading to cell death is involved in the process of fibrosis development during idiopathic pulmonary fibrosis (IPF). Among regulated/programmed cell death, the excessive apoptosis of AECs has been widely implicated in IPF pathogenesis. Necroptosis is a type of regulated/programmed necrosis. A multiprotein complex composed of receptor-interacting protein kinase (RIPK)-1 and -3 plays a key regulatory role in initiating necroptosis. Although necroptosis participates in disease pathogeneses through the release of damage-associated molecular patterns, its association with IPF progression remains elusive. In this study, we attempted to illuminate the involvement of RIPK3-regulated necroptosis in IPF pathogenesis. IPF lung tissues were used to detect necroptosis, and the role of RIPK3 was determined using cell culturing models of AECs. Lung fibrosis models of bleomycin (BLM) treatment were also used. RIPK3 expression levels were increased in IPF lungs, and both apoptosis and necroptosis were detected mainly in AECs. Necrostatin-1 and RIPK3 knockout experiments in AECs revealed the participation of necroptosis in BLM and hydrogen peroxide-induced cell death. BLM treatment induced RIPK3 expression in AECs and increased high-mobility group box 1 and IL-1β levels in mouse lungs. The efficient attenuation of BLM-induced lung inflammation and fibrosis was determined in RIPK3 knockout mice and by necrostatin-1 with a concomitant reduction in high-mobility group box 1 and IL-1β. RIPK3-regulated necroptosis in AECs is involved in the mechanism of lung fibrosis development through the release of damage-associated molecular patterns as part of the pathogenic sequence of IPF. |
| تدمد: | 1535-4989 1044-1549 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::628e6e52d56d96daf1247c27d3c1a5e9Test https://doi.org/10.1165/rcmb.2017-0034ocTest |
| رقم الانضمام: | edsair.doi.dedup.....628e6e52d56d96daf1247c27d3c1a5e9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15354989 10441549 |
|---|