Evaluation for Synergistic Effects by Combinations of Photodynamic Therapy (PDT) with Temoporfin (mTHPC) and Pt(II) Complexes Carboplatin, Cisplatin or Oxaliplatin in a Set of Five Human Cancer Cell Lines
| العنوان: | Evaluation for Synergistic Effects by Combinations of Photodynamic Therapy (PDT) with Temoporfin (mTHPC) and Pt(II) Complexes Carboplatin, Cisplatin or Oxaliplatin in a Set of Five Human Cancer Cell Lines |
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| المؤلفون: | Patrick J. Bednarski, Carsten Lange |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 19, Iss 10, p 3183 (2018) Volume 19 Issue 10 |
| بيانات النشر: | MDPI, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, combination assay, medicine.medical_treatment, cisplatin, Photodynamic therapy, Apoptosis, Carboplatin, lcsh:Chemistry, chemistry.chemical_compound, 0302 clinical medicine, Cytotoxicity, lcsh:QH301-705.5, Spectroscopy, Drug Synergism, General Medicine, Computer Science Applications, photodynamic therapy, Mesoporphyrins, 030220 oncology & carcinogenesis, cancer therapy, medicine.drug, Cell Survival, DNA Fragmentation, Phosphatidylserines, Catalysis, Article, Exocytosis, Inorganic Chemistry, Temoporfin, 03 medical and health sciences, Inhibitory Concentration 50, synergism, Cell Line, Tumor, medicine, Humans, MTT assay, Physical and Theoretical Chemistry, Molecular Biology, Cisplatin, Organic Chemistry, oxaliplatin, Cancer, Cell Cycle Checkpoints, medicine.disease, Oxaliplatin, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, chemistry, Photochemotherapy, Cancer research, Reactive Oxygen Species, mTHPC, Pt(II) complexes |
| الوصف: | The platinum(II) complexes carboplatin (CBDCA), cisplatin (CDDP) and oxaliplatin (1-OHP) are used as anticancer drugs in a large number of tumour chemotherapy regimens. Many attempts have been made to combine Pt(II)-based chemotherapy with alternative treatment strategies. One such alternative anticancer approach is known as photodynamic therapy (PDT), where a non-toxic photosensitizer (PS) produces oxidative stress via the formation of reactive oxygen species (ROS) after local illumination of the affected tissue. A very promising PS is 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (mTHPC, Temoporfin), which is approved for the treatment of head and neck cancer in Europe. In the present study, a combination of mTHPC-mediated PDT and either CBDCA, CDDP, or 1-OHP was applied to five human cancer cell lines from different tumour origins. Cytotoxicity was determined by the MTT assay and synergistic effects on cytotoxicity were evaluated by calculation of Combination Indices (CI). Synergy was identified in some of the combinations, for example, with 1-OHP in three of the tested cell lines but antagonism was also observed for a number of combinations in certain cell lines. In cases of synergy, elevated ROS levels were observed after combination but apoptosis induction was not necessarily increased compared to a treatment with a single compound. Cell cycle analysis revealed a formation of apoptotic subG1 populations and S phase as well as G2/M phase arrests after combination. In conclusion, pre-treatment with mTHPC-PDT has the potential to sensitize some types of tumour cells towards Pt(II) complexes, in particular 1-OHP but synergy is highly dependent on the type of cancer. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0a75c2572265281add91180b900fe22Test http://europepmc.org/articles/PMC6214074Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b0a75c2572265281add91180b900fe22 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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