التفاصيل البيبلوغرافية
| العنوان: |
环状 RNA hsa-circ-0001360 在同型半胱氨酸诱导人脐静脉内皮细胞凋亡中的作用. (Chinese) |
| العنوان البديل: |
Effect of cyclic RNA hsa-circ-0001360 on homocysteine-induced apoptosis of human umbilical vein endothelial cells. (English) |
| المؤلفون: |
况园军, 于素美, 钟颖怡, 章旭红, 马胜超, 杨安宁, 郝银菊, 熊建团, 焦 运, 姜怡邓 |
| المصدر: |
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu; 9/8/2024, Vol. 28 Issue 25, p4060-4064, 5p |
| الملخص (بالإنجليزية): |
BACKGROUND: Increased homocysteine level induces apoptosis of human umbilical vein endothelial cells, but the mechanism remains unclear. OBJECTIVE: To investigate the role of hsa-circ-0001360 in human umbilical vein endothelial cell apoptosis induced by homocysteine. METHODS: In vitro cultured human umbilical vein endothelial cells were divided into control group, homocysteine group, interference control group, interference control + homocysteine group, hsa-circ-0001360 interference group, hsa-circ-0001360 + homocysteine interference group, overexpression control group, overexpression control + homocysteine group, hsa-circ-0001360 overexpression group and hsa-circ-0001360 + homocysteine overexpression group. All groups were treated with 100 μmol/L homocysteine. After 72 hours of intervention, the expressions of apoptosis-related proteins Bax, Bcl-2, and Caspase-3 were detected by western blot assay. The apoptotic rate was detected by flow cytometry. Quantitative real-time PCR was used to detect the expression of hsacirc-0001360. RESULTS AND CONCLUSION: (1) Compared with the control group, the expression of Caspase-3 and Bax was significantly increased (P < 0.01), and the expression of Bcl-2 was significantly decreased (P < 0.01), and the apoptotic rate was significantly increased (P < 0.01) in the homocysteine group. (2) Compared with control group, the expression of hsa-circ-0001360 was significantly increased in the homocysteine group (P < 0.01). (3) The expression of hsa-circ-0001360 was significantly higher in the cytoplasm than that in the nucleus (P < 0.01). (4) Compared with the interference control C group and interference control + homocysteine group, the expressions of Caspase-3 and Bax were significantly decreased (P < 0.01), while the expression of Bcl-2 was significantly increased (P < 0.01); the apoptotic rate was significantly decreased (P < 0.01) in sh-hsa-circ-0001360 interference group and sh-hsa-circ-0001360 + homocysteine interference group. (5) Compared with overexpression control group and overexpression control + homocysteine group, the expressions of Caspase-3 and Bax were significantly increased (P < 0.01), while the expression of Bcl-2 was significantly decreased (P < 0.01); the apoptotic rate was significantly increased (P < 0.01) in the hsa-circ-0001360 overexpression group and the hsa-circ-0001360 + homocysteine overexpression group. (6) In conclusion, hsa-circ-0001360 can promote the apoptosis of human umbilical vein endothelial cells induced by homocysteine. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
背景：同型半胱氨酸水平上升会诱导人脐静脉内皮细胞发生凋亡，但机制尚不清楚。 目的：探讨hsa-circ-0001360在同型半胱氨酸诱导人脐静脉内皮细胞发生凋亡中的作用。 方法：体外培养人脐静脉内皮细胞，将其分为对照组、同型半胱氨酸组、干扰对照组、干扰对照+同型半胱氨酸组、干扰hsa-circ-0001360 组、干扰hsa-circ-0001360+同型半胱氨酸组、过表达对照组、过表达对照+同型半胱氨酸组、过表达hsa-circ-0001360组和过表达 hsa-circ-0001360+同型半胱氨酸组，同型半胱氨酸的干预浓度均为100 μmol/L。干预细胞72 h后，应用Western blot检测凋亡相关蛋白Bax、 Bcl-2和Caspase-3的表达；流式细胞仪检测细胞的凋亡率；实时荧光定量PCR(qRT-PCR)检测hsa-circ-0001360的表达水平。 结果与结论：①与对照组相比，同型半胱氨酸组人脐静脉内皮细胞中Caspase-3和Bax的表达明显升高(P < 0.01)，Bcl-2的表达明显降低( P < 0.01)，细胞凋亡率明显增高(P < 0.01)；②与对照组相比，同型半胱氨酸组人脐静脉内皮细胞中hsa-circ-0001360的表达明显升高(P < 0.01)； ③hsa-circ-0001360在人脐静脉内皮细胞的细胞质中表达显著高于细胞核(P < 0.01)；④与干扰对照组或干扰对照+同型半胱氨酸组相比，干 扰hsa-circ-0001360组和干扰hsa-circ-0001360+同型半胱氨酸组人脐静脉内皮细胞中Caspase-3、Bax的表达明显降低(P < 0.01)，Bcl-2的表达明 显升高(P < 0.01)，细胞凋亡率明显降低(P < 0.01)；⑤与过表达对照组或过表达对照+同型半胱氨酸组相比，过表达hsa-circ-0001360组和过 表达hsa-circ-0001360+同型半胱氨酸组人脐静脉内皮细胞中Caspase-3、Bax的表达明显升高(P < 0.01)，Bcl-2的表达明显降低(P < 0.01)，细胞 凋亡率明显升高(P < 0.01)；⑥以上结果表明，hsa-circ-0001360可以促进同型半胱氨酸诱导人脐静脉内皮细胞发生凋亡。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
