Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)
| العنوان: | Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea) |
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| المؤلفون: | Juan Carlos Navarro, Qinghui Ai, Kai Liao, Douglas R. Tocher, Kangsen Mai, Yuhui Yuan, Francisco Hontoria, Wei Xu, Songlin Li, Óscar Monroig, Tianjiao Wang |
| المساهمون: | National Natural Science Foundation of China, Ministerio de Economía y Competitividad (España), Monroig, Óscar, Navarro, Juan Carlos, Hontoria, Francisco, Tocher, Douglas R., Monroig, Óscar [0000-0001-8712-0440], Navarro, Juan Carlos [0000-0001-6976-6686], Hontoria, Francisco [0000-0003-2466-1375], Tocher, Douglas R. [0000-0002-8603-9410] |
| المصدر: | Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017) Digital.CSIC. Repositorio Institucional del CSIC instname Scientific Reports |
| بيانات النشر: | Springer Nature, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Fish Proteins, 0301 basic medicine, DNA, Complementary, Fatty Acid Elongases, Sequence analysis, Science, Article, Gene Expression Regulation, Enzymologic, 03 medical and health sciences, Acetyltransferases, Complementary DNA, Transcriptional regulation, Animals, Humans, Larimichthys crocea, Coding region, Amino Acid Sequence, Cloning, Molecular, Peptide sequence, Gene, Cells, Cultured, Phylogeny, Regulation of gene expression, Multidisciplinary, Sequence Homology, Amino Acid, biology, food and beverages, Sequence Analysis, DNA, biology.organism_classification, Perciformes, Isoenzymes, HEK293 Cells, 030104 developmental biology, Biochemistry, Fatty Acids, Unsaturated, Medicine, lipids (amino acids, peptides, and proteins) |
| الوصف: | In the present study, two elongases, Elovl4 and Elovl5, were functionally characterized and their transcriptional regulation in response to n-3 LC-PUFA administration were investigated in vivo and in vitro. We previously described the molecular characterization of croaker elovl5. Here, we report the full-length cDNA sequence of croaker elovl4, which contained 1794 bp (excluding the polyA tail), including 909 bp of coding region that encoded a polypeptide of 302 amino acids possessing all the characteristic features of Elovl proteins. Functional studies showed that croaker Elovl5, displayed high elongation activity towards C18 and C20 PUFA, with only low activity towards C22 PUFA. In contrast, croaker Elovl4 could effectively convert both C20 and C22 PUFA to longer polyenoic products up to C34. n-3 LC-PUFA suppressed transcription of the two elongase genes, as well as srebp-1 and lxrα, major regulators of hepatic lipid metabolism. The results of dual-luciferase reporter assays and in vitro studies both indicated that the transcriptions of elovl5 and elovl4 elongases could be regulated by Lxrα. Moreover, Lxrα could mediate the transcription of elovl4 directly or indirectly through regulating the transcription of srebp-1. The above findings contribute further insight and understanding of the mechanisms regulating LC-PUFA biosynthesis in marine fish species. This work was funded by the National Science Fund for Distinguished Young Scholars of China (31525024) and Spanish Government through the LONGFAQUA project (AGL2013-40986-R). We are grateful to prof. Qicun Zhou for their selfless assistance in vitro study. Additionally, we thank Xiaojing Dong for her help in promoter study, and Jianlong Du and Hanlin Xu for their help in fish rearing. |
| اللغة: | English |
| تدمد: | 3152-5024 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83df1135ff2e51f6fabc6cb45fa8ff0cTest http://hdl.handle.net/10261/190048Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....83df1135ff2e51f6fabc6cb45fa8ff0c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 31525024 |
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