دورية أكاديمية
AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny
العنوان: | AcMNPV Core Gene ac109 Is Required for Budded Virion Transport to the Nucleus and for Occlusion of Viral Progeny |
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المؤلفون: | Alfonso, Victoria, Maroniche, Guillermo Andrés, Reca, Sol Rita, Lopez, Maria Gabriela, Del Vas, Mariana, Taboga, Oscar Alberto |
المصدر: | PLoS ONE 7 (9) : e46146. (2012) |
بيانات النشر: | Plos One |
سنة النشر: | 2012 |
المجموعة: | Inta Digital (ID - Instituto Nacional de Tecnología Agropecuaria) |
مصطلحات موضوعية: | Lepidoptera, Autographa californica, Genética, Fenotipos, Virus, Genetics, Phenotypes, Viruses |
الوصف: | The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ac109 core gene has been previously characterized as an essential late gene. Our results showed that budded virions could be detected in supernatants of infected Sf-9 cells, even when ac109 knockout viruses displayed a single-cell infection phenotype. Moreover, confocal microscopy analysis revealed that budded virions can enter the cytoplasm but are unable to enter the cell nucleus. This defect could be repaired by complementing ac109 in trans. In addition, polyhedra of normal size could be detected in Sf-9 nuclei infected with ac109 knockout viruses. However, electron microscopy demonstrated that these occlusion bodies were empty. Altogether, these results indicate that ac109 is required for infectivity of both phenotypes of virus. ; Instituto de Biotecnología ; Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina ; Fil: Reca, Sol Rita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. ; Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina ; Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | application/pdf |
اللغة: | English |
تدمد: | 1932-6203 |
العلاقة: | info:eu-repograntAgreement/INTA/AERG/232161/AR./Vacunas a subunidades: optimización de la presentación antigénica e inmunomodulación y de los métodos biotecnológicos de producción; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046146Test; http://hdl.handle.net/20.500.12123/5117Test; https://doi.org/10.1371/journal.pone.0046146Test |
DOI: | 10.1371/journal.pone.0046146 |
الإتاحة: | https://doi.org/20.500.12123/5117Test https://doi.org/10.1371/journal.pone.0046146Test https://hdl.handle.net/20.500.12123/5117Test https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046146Test |
حقوق: | info:eu-repo/semantics/openAccess |
رقم الانضمام: | edsbas.73899FF6 |
قاعدة البيانات: | BASE |
تدمد: | 19326203 |
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DOI: | 10.1371/journal.pone.0046146 |