المؤلفون: Meropi Mari, Konstantinos Palikaras, George Filippidis, Costas Fotakis, Barbara Petanidou, Nektarios Tavernarakis

المصدر: Advanced Microscopy Techniques IV; and Neurophotonics II.

مصطلحات موضوعية: biology, medicine.diagnostic_test, Somatic cell, Nile red, Anatomy, biology.organism_classification, Cell biology, Green fluorescent protein, Pharyngeal muscles, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, In vivo, Microscopy, medicine, Lipid profile, Caenorhabditis elegans

الوصف: The non-linear techniques Second and Third Harmonic Generation (SHG, THG) have been employed simultaneously to record three dimensional (3D) imaging and localize the lipid content of the muscular areas (ectopic fat) of Caenorhabditis elegans (C. elegans). Simultaneously, Two-Photon Fluorescence (TPEF) was used initially to localize the stained lipids with Nile Red, but also to confirm the THG potential to image lipids successfully. In addition, GFP labelling of the somatic muscles, proves the initial suggestion of the existence of ectopic fat on the muscles and provides complementary information to the SHG imaging of the pharynx. The ectopic fat may be related to a complex of pathological conditions including type-2 diabetes, hypertension and cardiovascular diseases. The elucidation of the molecular path leading to the development of metabolic syndrome is a vital issue with high biological significance and necessitates accurate methods competent of monitoring lipid storage distribution and dynamics in vivo. THG microscopy was employed as a quantitative tool to monitor the lipid accumulation in non-adipose tissues in the pharyngeal muscles of 12 unstained specimens while the SHG imaging revealed the anatomical structure of the muscles. The ectopic fat accumulation on the pharyngeal muscles increases in wild type (N2) C. elegans between 1 and 9 days of adulthood. This suggests a correlation of the ectopic fat accumulation with the aging. Our results can provide new evidence relating the deposition of ectopic fat with aging, but also validate SHG and THG microscopy modalities as new, non-invasive tools capable of localizing and quantifying selectively lipid accumulation and distribution.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::458aa824f18b73c583edbec2a0c4e646Test
https://doi.org/10.1364/ecbo.2015.953613Test

المؤلفون: Susana I. C. O. Santos, Aleksandar J. Krmpot, M. Vlachos, Nektarios Tavernarakis, George Filippidis, David Artigas, G. J. Tserevelakis, Rodrigo Aviles-Espinosa, Wilhelm Kaenders, Andreas Brodschelm, Pablo Loza-Alvarez

المصدر: Biomedical Optics and 3-D Imaging.

مصطلحات موضوعية: Cell division, business.industry, Tissue characterization, Biology, Laser, Fluorescence, law.invention, Optics, law, Microscopy, Cell tracking, Third Harmonic Generation Microscopy, business, Scanning microscopy

الوصف: C. elegans embryogenesis, at the cell division stage, was imaged using third harmonic generation microscopy employing ultrashort pulsed lasers at 1028nm and 1550nm. This technique could be used for cell tracking studies without fluorescent markers.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::687ad22efb54bac8f0a8f2e4d2edbbb8Test
https://doi.org/10.1364/biomed.2010.btud78Test