Molecular Cloning, Bioinformatics Analysis and Expression of Insulin-Like Growth Factor 2 from Tianzhu White Yak, Bos grunniens
| العنوان: | Molecular Cloning, Bioinformatics Analysis and Expression of Insulin-Like Growth Factor 2 from Tianzhu White Yak, Bos grunniens |
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| المؤلفون: | Jishang Gong, Quanwei Zhang, Xingxu Zhao, Yong Zhang, Yalan Li, Xueying Wang, Youji Ma, Xiaohu Wu |
| المصدر: | International Journal of Molecular Sciences Volume 15 Issue 1 Pages 504-524 International Journal of Molecular Sciences, Vol 15, Iss 1, Pp 504-524 (2014) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | animal structures, endocrine system diseases, sequence analysis, Sequence analysis, rapid amplification of cDNA ends (RACE), Molecular Sequence Data, Sequence alignment, Molecular cloning, Biology, Molecular Dynamics Simulation, Catalysis, Protein Structure, Secondary, Article, Inorganic Chemistry, lcsh:Chemistry, Insulin-Like Growth Factor II, Complementary DNA, Coding region, Animals, Humans, Amino Acid Sequence, Physical and Theoretical Chemistry, Cloning, Molecular, Molecular Biology, Peptide sequence, Gene, lcsh:QH301-705.5, Spectroscopy, Phylogeny, Genetics, Binding Sites, Tianzhu white yak, Base Sequence, Organic Chemistry, IGF2 gene, Computational Biology, General Medicine, bioinformatics, Computer Science Applications, Protein Structure, Tertiary, Open reading frame, lcsh:Biology (General), lcsh:QD1-999, Cattle, Sequence Alignment |
| الوصف: | The IGF family is essential for normal embryonic and postnatal development and plays important roles in the immune system, myogenesis, bone metabolism and other physiological functions, which makes the study of its structure and biological characteristics important. Tianzhu white yak (Bos grunniens) domesticated under alpine hypoxia environments, is well adapted to survive and grow against severe hypoxia and cold temperatures for extended periods. In this study, a full coding sequence of the IGF2 gene of Tianzhu white yak was amplified by reverse transcription PCR and rapid-amplification of cDNA ends (RACE) for the first time. The cDNA sequence revealed an open reading frame of 450 nucleotides, encoding a protein with 179 amino acids. Its expression in different tissues was also studied by Real time PCR. Phylogenetic tree analysis indicated that yak IGF2 was similar to Bos taurus, and 3D structure showed high similarity with the human IGF2. The putative full CDS of yak IGF2 was amplified by PCR in five tissues, and cDNA sequence analysis showed high homology to bovine IGF2. Moreover the super secondary structure prediction showed a similar 3D structure with human IGF2. Its conservation in sequence and structure has facilitated research on IGF2 and its physiological function in yak. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms15010504 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2943d54382716afc7b572bfb5be19a4eTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....2943d54382716afc7b572bfb5be19a4e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms15010504 |