Molecular Characterization and Comparative Sequence Analysis of Defense-Related Gene, Oryza rufipogon Receptor-Like Protein Kinase 1
| العنوان: | Molecular Characterization and Comparative Sequence Analysis of Defense-Related Gene, Oryza rufipogon Receptor-Like Protein Kinase 1 |
|---|---|
| المؤلفون: | Beng Kah Song, R Wickneswari V Ratnam, Jennifer Ann Harikrishna, Ranganath Gudimella, Yee-Song Law |
| المصدر: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 13; Issue 7; Pages: 9343-9362 |
| بيانات النشر: | Molecular Diversity Preservation International (MDPI), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Subfamily, disease resistance, Sequence analysis, Molecular Sequence Data, Leucine-rich repeat, Genes, Plant, Polymorphism, Single Nucleotide, Catalysis, Article, Inorganic Chemistry, Complementary DNA, Oryza rufipogon, RPK1, LRR domain, kinase domain, Coding region, Physical and Theoretical Chemistry, Molecular Biology, Gene, Spectroscopy, Plant Proteins, Genetics, Oryza sativa, biology, Base Sequence, Organic Chemistry, fungi, food and beverages, Oryza, General Medicine, Exons, biology.organism_classification, Computer Science Applications, Protein Kinases |
| الوصف: | Many of the plant leucine rich repeat receptor-like kinases (LRR-RLKs) have been found to regulate signaling during plant defense processes. In this study, we selected and sequenced an LRR-RLK gene, designated as Oryza rufipogon receptor-like protein kinase 1 (OrufRPK1), located within yield QTL yld1.1 from the wild rice Oryza rufipogon (accession IRGC105491). A 2055 bp coding region and two exons were identified. Southern blotting determined OrufRPK1 to be a single copy gene. Sequence comparison with cultivated rice orthologs (OsI219RPK1, OsI9311RPK1 and OsJNipponRPK1, respectively derived from O. sativa ssp. indica cv. MR219, O. sativa ssp. indica cv. 9311 and O. sativa ssp. japonica cv. Nipponbare) revealed the presence of 12 single nucleotide polymorphisms (SNPs) with five non-synonymous substitutions, and 23 insertion/deletion sites. The biological role of the OrufRPK1 as a defense related LRR-RLK is proposed on the basis of cDNA sequence characterization, domain subfamily classification, structural prediction of extra cellular domains, cluster analysis and comparative gene expression. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f9d7e9c8b9fe00d3f486cdccf737222Test http://europepmc.org/articles/PMC3430300Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0f9d7e9c8b9fe00d3f486cdccf737222 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
|---|