مورد إلكتروني
Systematic cyanobacterial membrane proteome analysis by combining acid hydrolysis and digestive enzymes with nano-liquid chromatography-Fourier transform mass spectrometry
| العنوان: | Systematic cyanobacterial membrane proteome analysis by combining acid hydrolysis and digestive enzymes with nano-liquid chromatography-Fourier transform mass spectrometry |
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| بيانات النشر: | Institutionen för biokemi och biofysik 2010 |
| تفاصيل مُضافة: | Kwon, Joseph Oh, Jeehyun Park, Chiyoul Cho, Kun Kim, Seung Il Kim, Soohyun Lee, Sunghoon Bhak, Jong Norling, Birgitta Choi, Jejong-Soon |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The identification of membrane proteins is currently under-represented since the trans-membrane domains of membrane proteins have a hydrophobic property. Membrane proteins have mainly been analyzed by cleaving and identifying exposed hydrophilic domains. We developed the membrane proteomics method for targeting integral membrane proteins by the following sequential process: in-solution acid hydrolysis, reverse phase chromatographic separation, trypsin or chymotrypsin digestion and nano-liquid chromatography-Fourier transform mass spectrometry. When we employed total membrane proteins of Synechocystis sp. PCC 6803, 155 integral membrane proteins out of a predictable 706 were identified in a single application, corresponding to 22% of a genome. The combined methods of acid hydrolysis-trypsin (AT) and acid hydrolysis-chymotrypsin (AC) identified both hydrophilic and hydrophobic domains of integral membrane proteins, respectively. The systematic approach revealed a more concrete data in mapping the repertoire of cyanobacterial membrane and membrane-linked proteome. authorCount :10 |
| مصطلحات الفهرس: | Acid hydrolysis/chymotrypsin, Acid hydrolysis/trypsin, Synechocystis sp PCC 6803, Trans-membrane domain, Integral membrane protein, HPLC, nano-LC-MS, Natural Sciences, Naturvetenskap, Article in journal, info:eu-repo/semantics/article, text |
| DOI: | 10.1016.j.chroma.2009.11.045 |
| URL: | Journal of Chromatography A, 0021-9673, 2010, 1217:3, s. 285-293 |
| الإتاحة: | Open access content. Open access content info:eu-repo/semantics/restrictedAccess |
| ملاحظة: | English |
| أرقام أخرى: | UPE oai:DiVA.org:su-50315 doi:10.1016/j.chroma.2009.11.045 ISI:000273912300006 1234796752 |
| المصدر المساهم: | UPPSALA UNIV LIBR From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1234796752 |
| قاعدة البيانات: | OAIster |
| DOI: | 10.1016.j.chroma.2009.11.045 |
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