دورية أكاديمية
Serum proteomic profiles in CKCS with Mitral valve disease
| العنوان: | Serum proteomic profiles in CKCS with Mitral valve disease |
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| المؤلفون: | C. Locatelli, C. Piras, RISCAZZI, GIULIA, I. Alloggio, SPALLA, ILARIA, A. Soggiu, L. Bonizzi, P.G. Brambilla, V. Greco, P. Roncada |
| المساهمون: | C. Locatelli, C. Pira, G. Riscazzi, I. Alloggio, I. Spalla, A. Soggiu, V. Greco, L. Bonizzi, P. Roncada, P.G. Brambilla |
| بيانات النشر: | BioMed Central Ltd. |
| سنة النشر: | 2017 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | Cavalier King Charles Spaniel, Dog, Mitral valve disease, Proteomic analysi, Animal, Blood Protein, Breeding, Case-Control Studie, Dog Disease, Female, Heart Valve Disease, Male, Mitral Valve, Proteomic, Proteome, Veterinary (all), Settore VET/08 - Clinica Medica Veterinaria, Settore VET/05 - Malattie Infettive degli Animali Domestici, Settore MED/07 - Microbiologia e Microbiologia Clinica, Settore BIO/10 - Biochimica |
| الوصف: | Background: Myxomatous mitral valve disease (MVD) is the most common acquired heart disease in dogs, and the Cavalier King Charles Spaniel (CKCS) is the most studied breed because of the high prevalence, early onset and hereditary component evidenced in the breed. MVD has different severity levels, and there are many practical limitations in identifying its asymptomatic stages. Proteomic techniques are valuable for studying the proteins and peptides involved in cardiovascular diseases, including the period prior to the clinical onset of the disease. The aim of this study was to identify the serum proteins that were differentially expressed in healthy CKCS and those affected by MVD in mild to severe stages. Proteomics analysis was performed using two-dimensional gel electrophoresis separation and a bioinformatics analysis for the detection of differentially expressed spots. In a comparative analysis, protein spots with a p<0.05 (ANOVA) were considered statistically significant and were excised from the gels for analysis by MALDI-TOF-MS for protein identification. Results: Eight proteins resulted differentially expressed among the groups and significantly related to the progression of the disease. In mild affected group versus healthy dogs complement factor H isoform 2, inhibitor of carbonic anhydrase, hemopexin, dystrobrevin beta isoform X7 and CD5 molecule-like resulted to be down-regulated, whereas fibronectin type-III domain-containing protein 3A isoform X4 was up-regulated. In severe affected dogs versus healthy group complement factor H isoform 2, calpain-3 isoform X2, dystrobrevin beta isoform X7, CD5 molecule-like and l-2-hydroxyglutarate dehydrogenase resulted to be down-regulated. Complement factor H isoform 2, calpain-3 isoform X2, dystrobrevin beta isoform X7, CD5 molecule-like and hydroxyglutarate dehydrogenase were found to be down-regulated in mild affected group versus healthy dogs. All of these proteins except complement factor H followed a decreasing trend according to the progression of the ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/28173805; info:eu-repo/semantics/altIdentifier/wos/WOS:000394604100001; volume:13; issue:1; firstpage:43; numberofpages:9; journal:BMC VETERINARY RESEARCH; http://hdl.handle.net/2434/479282Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85011824769; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297119Test/ |
| DOI: | 10.1186/s12917-017-0951-5 |
| الإتاحة: | https://doi.org/10.1186/s12917-017-0951-5Test http://hdl.handle.net/2434/479282Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297119Test/ |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.64584BBA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12917-017-0951-5 |
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