دورية أكاديمية
Paenibacillus infection with frequent viral coinfection contributes to postinfectious hydrocephalus in Ugandan infants
| العنوان: | Paenibacillus infection with frequent viral coinfection contributes to postinfectious hydrocephalus in Ugandan infants |
|---|---|
| المؤلفون: | Paulson, Joseph N, Williams, Brent L, Hehnly, Christine, Mishra, Nischay, Sinnar, Shamim A, Zhang, Lijun, Ssentongo, Paddy, Mbabazi-Kabachelor, Edith, Wijetunge, Dona SS, von Bredow, Benjamin, Mulondo, Ronnie, Kiwanuka, Julius, Bajunirwe, Francis, Bazira, Joel, Bebell, Lisa M, Burgoine, Kathy, Couto-Rodriguez, Mara, Ericson, Jessica E, Erickson, Tim, Ferrari, Matthew, Gladstone, Melissa, Guo, Cheng, Haran, Murali, Hornig, Mady, Isaacs, Albert M, Kaaya, Brian Nsubuga, Kangere, Sheila M, Kulkarni, Abhaya V, Kumbakumba, Elias, Li, Xiaoxiao, Jr, Limbrick David D, Magombe, Joshua, Morton, Sarah U, Mugamba, John, Ng, James, Olupot-Olupot, Peter, Onen, Justin, Peterson, Mallory R, Roy, Farrah, Sheldon, Kathryn, Townsend, Reid, Weeks, Andrew D, Whalen, Andrew J, Quackenbush, John, Ssenyonga, Peter, Galperin, Michael Y, Almeida, Mathieu, Atkins, Hannah, Warf, Benjamin C, Lipkin, W Ian, Broach, James R, Schiff, Steven J |
| بيانات النشر: | American Association for the Advancement of Science (AAAS) |
| سنة النشر: | 2020 |
| المجموعة: | The University of Liverpool Repository |
| الوصف: | Postinfectious hydrocephalus (PIH), which often follows neonatal sepsis, is the most common cause of pediatric hydrocephalus worldwide, yet the microbial pathogens underlying this disease remain to be elucidated. Characterization of the microbial agents causing PIH would enable a shift from surgical palliation of cerebrospinal fluid (CSF) accumulation to prevention of the disease. Here, we examined blood and CSF samples collected from 100 consecutive infant cases of PIH and control cases comprising infants with non-postinfectious hydrocephalus in Uganda. Genomic sequencing of samples was undertaken to test for bacterial, fungal, and parasitic DNA; DNA and RNA sequencing was used to identify viruses; and bacterial culture recovery was used to identify potential causative organisms. We found that infection with the bacterium Paenibacillus, together with frequent cytomegalovirus (CMV) coinfection, was associated with PIH in our infant cohort. Assembly of the genome of a facultative anaerobic bacterial isolate recovered from cultures of CSF samples from PIH cases identified a strain of Paenibacillus thiaminolyticus. This strain, designated Mbale, was lethal when injected into mice in contrast to the benign reference Paenibacillus strain. These findings show that an unbiased pan-microbial approach enabled characterization of Paenibacillus in CSF samples from PIH cases, and point toward a pathway of more optimal treatment and prevention for PIH and other proximate neonatal infections. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | http://livrepository.liverpool.ac.uk/3104136/1/Paulson%20Sci%20Trans%20Med%20preprint.pdfTest; Collapse authors list. Paulson, Joseph N, Williams, Brent L, Hehnly, Christine, Mishra, Nischay, Sinnar, Shamim A, Zhang, Lijun, Ssentongo, Paddy, Mbabazi-Kabachelor, Edith, Wijetunge, Dona SS, von Bredow, Benjamin et al (show 42 more authors) , Mulondo, Ronnie, Kiwanuka, Julius, Bajunirwe, Francis, Bazira, Joel, Bebell, Lisa M, Burgoine, Kathy orcid:0000-0001-7975-745X , Couto-Rodriguez, Mara, Ericson, Jessica E, Erickson, Tim, Ferrari, Matthew, Gladstone, Melissa orcid:0000-0002-2579-9301 , Guo, Cheng, Haran, Murali, Hornig, Mady, Isaacs, Albert M, Kaaya, Brian Nsubuga, Kangere, Sheila M, Kulkarni, Abhaya V, Kumbakumba, Elias, Li, Xiaoxiao, Jr, Limbrick David D, Magombe, Joshua, Morton, Sarah U, Mugamba, John, Ng, James, Olupot-Olupot, Peter, Onen, Justin, Peterson, Mallory R, Roy, Farrah, Sheldon, Kathryn, Townsend, Reid, Weeks, Andrew D orcid:0000-0002-1909-337X , Whalen, Andrew J, Quackenbush, John, Ssenyonga, Peter, Galperin, Michael Y, Almeida, Mathieu, Atkins, Hannah, Warf, Benjamin C, Lipkin, W Ian, Broach, James R and Schiff, Steven J (2020) Paenibacillus infection with frequent viral coinfection contributes to postinfectious hydrocephalus in Ugandan infants. SCIENCE TRANSLATIONAL MEDICINE, 12 (563). eaba0565-. |
| الإتاحة: | http://livrepository.liverpool.ac.uk/3104136Test/ https://stm.sciencemag.org/content/12/563/eaba0565Test http://livrepository.liverpool.ac.uk/3104136/1/Paulson%20Sci%20Trans%20Med%20preprint.pdfTest |
| رقم الانضمام: | edsbas.DA0A6848 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |