دورية أكاديمية
Inhibition of murine neutrophil recruitment in vivo by CXC chemokine receptor antagonists
| العنوان: | Inhibition of murine neutrophil recruitment in vivo by CXC chemokine receptor antagonists |
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| المؤلفون: | McColl, S., Clark-Lewis, I. |
| بيانات النشر: | AMER ASSOC IMMUNOLOGISTS |
| سنة النشر: | 1999 |
| المجموعة: | The University of Adelaide: Digital Library |
| مصطلحات موضوعية: | Leukocytes, Neutrophils, Animals, Mice, Inbred BALB C, Peritonitis, Platelet Factor 4, Growth Substances, Intercellular Signaling Peptides and Proteins, Peptide Fragments, Receptors, Chemokine, Chemokines, CXC, Chemotactic Factors, Immune Sera, Monokines, Cell Migration Inhibition, Immunization, Passive, Diffusion Chambers, Culture, Injections, Intraperitoneal, Cell Movement, Male, Chemokine CXCL1, Chemokine CXCL2 |
| الوصف: | In this study, we have examined the ability of chemokine receptor antagonists to prevent neutrophil extravasation in the mouse. Two murine CXC chemokines, macrophage-inflammatory protein (MIP)-2 and KC, stimulated the accumulation of leukocytes into s.c. air pouches, although MIP-2 was considerably more potent. The leukocyte infiltrate was almost exclusively neutrophilic in nature. A human CXC chemokine antagonist, growth-related oncogene (GRO)-alpha(8-73), inhibited calcium mobilization induced by MIP-2, but not by platelet-activating factor in leukocytes isolated from the bone marrow, indicating that this antagonist inhibits MIP-2 activity toward murine leukocytes. Pretreatment of mice with GROalpha(8-73) inhibited, in a dose-dependent manner, the MIP-2-induced influx of neutrophils to levels that were not significantly different from control values. Moreover, this antagonist was also effective in inhibiting the leukocyte recruitment induced by TNF-alpha, LPS, and IL-1beta. Leukocyte infiltration into the peritoneal cavity in response to MIP-2 was also inhibited by prior treatment of mice with GROalpha(8-73) or the analogue of platelet factor 4, PF4(9-70). The results of this study indicate 1) that the murine receptor for MIP-2 and KC, muCXCR2, plays a major role in neutrophil recruitment to s.c. tissue and the peritoneal cavity in response to proinflammatory agents and 2) that CXCR2 receptor antagonists prevent acute inflammation in vivo. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0022-1767 1550-6606 |
| العلاقة: | Journal of Immunology, 1999; 163(5):2829-2835; http://hdl.handle.net/2440/11625Test; McColl, S. [0000-0003-0949-4660] |
| الإتاحة: | http://hdl.handle.net/2440/11625Test |
| رقم الانضمام: | edsbas.65F576D9 |
| قاعدة البيانات: | BASE |
| تدمد: | 00221767 15506606 |
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