دورية أكاديمية
Bronchoalveolar lavage fluids of ventilated patients with acute lung injury activate NF-kB in alveolar epithelial cell line: role of reactive oxygen/nitrogen species and cytokines
| العنوان: | Bronchoalveolar lavage fluids of ventilated patients with acute lung injury activate NF-kB in alveolar epithelial cell line: role of reactive oxygen/nitrogen species and cytokines |
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| المؤلفون: | Nys, Monique, Deby, Ginette, Habraken, Yvette, Legrand-Poels, Sylvie, Kohnen, S., Ledoux, Didier, Canivet, Jean-Luc, Damas, Pierre, Lamy, Maurice |
| المصدر: | Nitric Oxide, 9 (1), 33-43 (2003) |
| بيانات النشر: | Academic Press |
| سنة النشر: | 2003 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | bronchoalveolar lavage, nuclear factor-kappa B, bronchopneumonia, ARDS, cytokines, reactive nitrogen/oxygen species, Human health sciences, Anesthesia & intensive care, Life sciences, Biochemistry, biophysics & molecular biology, Sciences de la santé humaine, Anesthésie & soins intensifs, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | peer reviewed ; In human alveolar epithelial cell line, we investigated the binding activity of NF-kappaB induced by the bronchoalveolar lavage fluids (BALs) from ventilated patients with acute lung injury (ALI), in correlation with the concentrations of inflammatory cytokines, RNOS, and the severity of the ALI. In BALs obtained in 67 patients (16 bronchopneumonia, 14 infected ARDS, 20 ARDS, and 17 ALI patients without bronchopneumonia and no ARDS), we measured endotoxin, IL-1beta, IL-8, and nitrated proteins (NTP), the activity of mycloperoxidase, and the capacity to activate the NF-kappaB in alveolar A549 cells by electrophoretic mobility shift and supershift assays. The neutrophil counts and mean IL-1beta, IL-8, myeloperoxidase, and NTP values were increased in bronchopneumonia and infected ARDS groups compared to ARDS and ALI without bronchopneumonia and no ARDS groups (P < 0.001). The number of neutrophils was correlated to those of IL-1beta, IL-8, myeloperoxidase, NTP, and endotoxin in all groups (P < 0.0001). NF-kappaB activity was induced in alveolar like cells by BALs in all groups, was higher in bronchopneumonia and infected ARDS groups (P < 0.02), and was correlated to IL-1beta (P = 0.0002), IL-8 (P = 0.02), NTP (P = 0.014), myeloperoxidase (P = 0.016), and neutrophil counts (P = 0.003). BALs of bronchopneumonia and infected ARDS patients had increased inflammatory mediators (compared to ARDS and ALI without bronchopneumonia and no ARDS patients) that correlated to neutrophil counts and to the NF-kappaB-binding activity. These mediators and NF-kappaB activation may induce an amplification of inflammatory phenomena. By in vitro studies, we confirmed that NO-derived species (10(-6) to 10(-5) M peroxynitrite and 10(-5) M nitrites) and myeloperoxidase (at concentration equivalent to that found in BALs) can participate in the NF-kappaB activation. (C) 2003 Elsevier Inc. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1089-8603 1089-8611 |
| العلاقة: | urn:issn:1089-8603; urn:issn:1089-8611; https://orbi.uliege.be/handle/2268/852Test; info:hdl:2268/852; scopus-id:2-s2.0-0141996271; info:pmid:14559430 |
| DOI: | 10.1016/j.niox.2003.07.001 |
| الإتاحة: | https://doi.org/10.1016/j.niox.2003.07.001Test https://orbi.uliege.be/handle/2268/852Test |
| حقوق: | restricted access ; http://purl.org/coar/access_right/c_16ecTest ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.9E5D6DBB |
| قاعدة البيانات: | BASE |
| تدمد: | 10898603 10898611 |
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| DOI: | 10.1016/j.niox.2003.07.001 |