Detection of pathogens by real-time PCR in adult patients with acute exacerbation of bronchial asthma
| العنوان: | Detection of pathogens by real-time PCR in adult patients with acute exacerbation of bronchial asthma |
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| المؤلفون: | Takanori Numata, Yutaka Yoshii, Kazuyoshi Kuwano, Kimiko Ubukata, Naoko Chiba, Hiroshi Wakui, Miyuki Morozumi, Shigeo Hanada, Hironori Uruga, Keisuke Saito, Hiromichi Hara, Kenichiro Shimizu, Jun Araya, Kazuma Kishi, Katsutoshi Nakayama, Shunsuke Minagawa |
| المصدر: | BMC Pulmonary Medicine, Vol 17, Iss 1, Pp 1-8 (2017) BMC Pulmonary Medicine |
| بيانات النشر: | BMC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, Rhinovirus, Exacerbation, medicine.disease_cause, Severity of Illness Index, Real-time polymerase chain reaction, Haemophilus influenzae, law.invention, 0302 clinical medicine, Risk Factors, law, Prospective Studies, 030212 general & internal medicine, Respiratory Tract Infections, Bronchial asthma, Polymerase chain reaction, Aged, 80 and over, Middle Aged, Anti-Bacterial Agents, Acute exacerbation, Disease Progression, Female, Seasons, medicine.symptom, Research Article, Adult, Pulmonary and Respiratory Medicine, Atypical bacteria, Antiviral Agents, Virus, Young Adult, 03 medical and health sciences, medicine, Humans, Aged, lcsh:RC705-779, business.industry, Pathogen, Sputum, lcsh:Diseases of the respiratory system, Asthma, 030228 respiratory system, Multivariate Analysis, Immunology, Risk factor, business |
| الوصف: | Background Respiratory tract infection is a major cause of acute exacerbation of bronchial asthma (AEBA). Although recent findings suggest that common bacteria are causally associated with AEBA, a comprehensive epidemiologic analysis of infectious pathogens including common/atypical bacteria and viruses in AEBA has not been performed. Accordingly, we attempted to detect pathogens during AEBA by using real-time polymerase chain reaction (PCR) in comparison to conventional methods. Methods We prospectively enroled adult patients with AEBA from August 2012 to March 2014. Infectious pathogens collected in nasopharyngeal swab and sputum samples were examined in each patient by conventional methods and real-time PCR, which can detect 6 bacterial and 11 viral pathogens. The causal association of these pathogens with AEBA severity and their frequency of monthly distribution were also examined. Results Among the 64 enroled patients, infectious pathogens were detected in 49 patients (76.6%) using real-time PCR and in 14 patients (21.9%) using conventional methods (p |
| اللغة: | English |
| تدمد: | 1471-2466 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9fbddf8a9c740f3d164d5bb5609a608Test http://link.springer.com/article/10.1186/s12890-017-0494-3Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e9fbddf8a9c740f3d164d5bb5609a608 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712466 |
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