Overexpression of the genes of glycerol catabolism and glycerol facilitator improves glycerol conversion to ethanol in the methylotrophic thermotolerant yeastOgataea polymorpha
| العنوان: | Overexpression of the genes of glycerol catabolism and glycerol facilitator improves glycerol conversion to ethanol in the methylotrophic thermotolerant yeastOgataea polymorpha |
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| المؤلفون: | Kostyantyn V. Dmytruk, Andriy A. Sibirny, Wladimir Sibirny, Marta V. Semkiv, Iwona Kata, Orysya Ternavska |
| المصدر: | Yeast. 36:329-339 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Glycerol, Thermotolerance, 0106 biological sciences, Dihydroxyacetone, Bioengineering, Ethanol fermentation, 01 natural sciences, Applied Microbiology and Biotechnology, Biochemistry, Oxidative Phosphorylation, Pichia, Pichia pastoris, 03 medical and health sciences, chemistry.chemical_compound, 010608 biotechnology, Genetics, Biomass, Food science, 030304 developmental biology, 0303 health sciences, Biodiesel, Ethanol, biology, biology.organism_classification, Yeast, chemistry, Fermentation, Saccharomycetales, Carbohydrate Metabolism, Ogataea polymorpha, Metabolic Networks and Pathways, Biotechnology |
| الوصف: | Production of fuel ethanol is one of the possible ways to utilize crude glycerol, substantial amounts of which are produced by biodiesel industry. Earlier, we have described construction of the recombinant strains of methylotrophic thermotolerant yeast Ogataea polymorpha with simultaneous overexpression of the genes PDC1 and ADH1, which produced increased amounts of ethanol from glycerol. In this work, we have further improved these strains by overexpression of genes involved either in oxidative (through dihydroxyacetone) or phosphorylative (through glycerol-3-phosphate) pathway of glycerol catabolism, as well as heterologous gene coding for glycerol transporter FPS1 from Komagataella phaffii (formerly, Pichia pastoris). Obtained recombinant strains produced up to 10.7 g/L of ethanol (with ethanol productivity 30 mg/g of biomass/hr and yield 132 mg/g of consumed glycerol) from pure glycerol and up to 3.55 g/L of ethanol (with ethanol productivity 11.6 mg/g of biomass/hr and yield 72.3 mg/g of consumed glycerol) from crude glycerol as a carbon source, which is approximately 15 times more relative to that of the O. polymorpha wild-type strain and 2.2 more relative to the earlier constructed strain. |
| تدمد: | 1097-0061 0749-503X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5393f21c103f6868607f725787b005bTest https://doi.org/10.1002/yea.3387Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....a5393f21c103f6868607f725787b005b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970061 0749503X |
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