Expression of yeast homolog of the mammalBCRPgene coding for riboflavin efflux protein activates vitamin B2production in the flavinogenic yeastCandida famata
| العنوان: | Expression of yeast homolog of the mammalBCRPgene coding for riboflavin efflux protein activates vitamin B2production in the flavinogenic yeast |
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| المؤلفون: | Daria V. Fedorovych, Lyubov R. Fayura, Andriy O. Tsyrulnyk, Justyna Ruchala, Yuliia A. Andreieva, Kostyantyn V. Dmytruk, Andriy A. Sibirny |
| المصدر: | Yeast. 37:467-473 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, Bioengineering, Riboflavin, 01 natural sciences, Applied Microbiology and Biotechnology, Biochemistry, 03 medical and health sciences, 010608 biotechnology, Debaryomyces hansenii, Genetics, Fluorescence microscope, heterocyclic compounds, Gene, 030304 developmental biology, 0303 health sciences, biology, Strain (chemistry), digestive, oral, and skin physiology, food and beverages, biology.organism_classification, Yeast, Specific activity, Efflux, human activities, Biotechnology |
| الوصف: | Candida famata is a representative of a group of so-called flavinogenic yeast species that overproduce riboflavin (vitamin B2 ) in response to iron limitation. Overproduced riboflavin accumulates in the cultural medium rather than in the cells suggesting existence of the special mechanisms involved in riboflavin excretion. The corresponding protein and gene have not been identified in yeasts. At the same time, the corresponding gene BCRP has been identified in mammal mammary glands. Several homologs of the mammal BCRP gene encoding putative riboflavin efflux protein (excretase) were identified in Debaryomyces hansenii. The closest homolog was expressed under the control of D. hansenii TEF1 promoter in the riboflavin overproducing strain of C. famata. Resulted transformants overexpressed the corresponding gene and produced 1.4- to 1.8-fold more riboflavin as compared with the parental strain. They also were characterized by overexpression of RIB1 and RIB6 genes of riboflavin synthesis and exhibited elevated specific activity of GTP-cyclohydrolase II. Membrane localization of the riboflavin excretase was confirmed by fluorescent microscopy. |
| تدمد: | 1097-0061 0749-503X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::6fb5acaa9ba92999791d8c0cff04f5ceTest https://doi.org/10.1002/yea.3470Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........6fb5acaa9ba92999791d8c0cff04f5ce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970061 0749503X |
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