التفاصيل البيبلوغرافية
| العنوان: |
Intra-abdominal adhesion formation induces anti-oxidative injury, enhances cell proliferation, and prevents complement-mediated lysis. |
| المؤلفون: |
Yu, Sung‐Liang, Singh, Sher, Chen, Huei‐Wen, Chen, Hsuan‐Yu, Chen, Jeremy J. W., Chen, Wen‐Jone, Chen, Han‐Shiang, Chen, Shyr‐Chyr |
| المصدر: |
Wound Repair & Regeneration; May/Jun2008, Vol. 16 Issue 3, p388-398, 11p, 1 Color Photograph, 3 Charts, 2 Graphs |
| مصطلحات موضوعية: |
WOUND care, WOUND healing, GENE expression, IMMUNOHISTOCHEMISTRY, CELL proliferation, CELL growth |
| مستخلص: |
Whether the alteration of gene expression is accompanied with intra-abdominal adhesion formation is unclear. The aim of this study was to analyze the dynamic gene expression patterns in an animal model of intra-abdominal adhesion formation. The mRNA was extracted from the jejunums of sham control mice and jejunum-abrading mice at 1, 3, 7, and 14 days postsurgery. The mouse cDNA microarray was used to monitor the dynamic changes of the tested genes and up-regulated and down-regulated genes were calculated. Quantitative real-time RT-PCR, and immunohistochemistry staining were used to confirm the accuracy of microarray results at RNA and protein levels. The top 100 genes with the greatest change across all studied mice groups were identified and 93 of them were correct after sequencing verification. Of the 93 genes, 74 genes were up-regulated and 19 were down-regulated following jejunal abrasion. Gene expressions of complement-mediated lysis, anti-oxidative response, and cell proliferation were significantly induced during adhesion formation. Intra-abdominal adhesion induces several genes to eliminate overfilled complement-mediated lysis, prevent oxidative injuries, and enhance cell proliferation. These findings may provide insights into the pathogenesis of intra-abdominal adhesion formation and might also help to identify some new target genes for specific diagnostic tools and novel therapeutic strategies. [ABSTRACT FROM AUTHOR] |
|
Copyright of Wound Repair & Regeneration is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| قاعدة البيانات: |
Complementary Index |
