دورية أكاديمية
Mechanistic Insights into Translational Silencing of HACL mRNA in Yeast S. Cerevisiae
| العنوان: | Mechanistic Insights into Translational Silencing of HACL mRNA in Yeast S. Cerevisiae |
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| المؤلفون: | Sathe, Leena |
| المصدر: | Theses and Dissertations |
| بيانات النشر: | UWM Digital Commons |
| سنة النشر: | 2019 |
| المجموعة: | University of Wisconsin-Milwaukee: UWM Digital Commons |
| مصطلحات موضوعية: | Biology, Molecular Biology |
| الوصف: | Protein synthesis is a fundamental life process. Protein synthesis regulates cellular metabolism, cellular growth, the cell cycle, and cell morphogenesis. Technical advances in molecular biology, advanced high-throughput sequencing technologies and recent developments in crystallographic methodologies have aided in better understanding of the process of protein synthesis. Current knowledge of protein synthesis provides us with an overview of the initiation, elongation, and termination steps, general regulatory mechanisms, and molecular functions of the ribosomal rRNA and proteins. However, even with all this information we are far from understanding the detailed sequence of molecular interactions involved in the process of protein synthesis. We have snapshots of different stages of protein synthesis (initiation, elongation, and termination) but, we are still missing the intricate details responsible for linking these snapshots and creating a complete picture depicting the mechanism of protein synthesis. This thesis is focused on understanding the mechanism of protein synthesis using the yeast HAC1 mRNA as a model mRNA in yeast Saccharomyces cerevisiae. The messenger RNA (mRNA) bears the genetic information that is decoded in the process of protein synthesis. The fate of a messenger mRNA is decided by regulatory elements present in the mRNA that are known as cis-acting elements. They influence not only translation of the mRNA, but also mRNA splicing, mRNA localization, mRNA processing, and mRNA degradation. One of the cis-regulating factors is the RNA secondary structure. The RNA secondary structure controls the translation of an mRNA. The precise mechanism of which is not clearly understood. This thesis is aimed at uncovering the regulation of protein synthesis by the RNA secondary structure. Proteins in their native conformation either spontaneously attain the folded conformation or they are folded by chaperone proteins in the cytoplasm or in the endoplasmic reticulum (ER). Sometimes the ER experiences an ... |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://dc.uwm.edu/etd/2245Test; https://dc.uwm.edu/context/etd/article/3250/viewcontent/Sathe_uwm_0263D_12468.pdfTest |
| الإتاحة: | https://dc.uwm.edu/etd/2245Test https://dc.uwm.edu/context/etd/article/3250/viewcontent/Sathe_uwm_0263D_12468.pdfTest |
| رقم الانضمام: | edsbas.298A21BE |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |