Validation strategies for antibodies targeting modified ribonucleotides
| العنوان: | Validation strategies for antibodies targeting modified ribonucleotides |
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| المؤلفون: | Stefan Canzar, Vigo Heissmeyer, Nicholas B. Angstman, Mark Helm, Aloys Schepers, Franziska Weichmann, Kaouthar Slama, Regina Feederle, Julian König, Robert Hett, Gunter Meister, Florian D. Hastert, M. Cristina Cardoso, Taku Ito-Kureha, Stefan Hüttelmaier, Andrew Flatley, Christoph Dieterich |
| المصدر: | RNA |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Regulation of gene expression, 0303 health sciences, Messenger RNA, biology, Nucleotides, medicine.drug_class, 030302 biochemistry & molecular biology, Method, Computational biology, Ribonucleotides, Monoclonal antibody, Antibodies, 03 medical and health sciences, Low affinity, chemistry, biology.protein, medicine, RNA, Nucleotide, RNA, Messenger, Antibody, Molecular Biology, 030304 developmental biology |
| الوصف: | Chemical modifications are found on almost all RNAs and affect their coding and noncoding functions. The identification of m6A on mRNA and its important role in gene regulation stimulated the field to investigate whether additional modifications are present on mRNAs. Indeed, modifications including m1A, m5C, m7G, 2′-OMe, and Ψ were detected. However, since their abundances are low and tools used for their corroboration are often not well characterized, their physiological relevance remains largely elusive. Antibodies targeting modified nucleotides are often used but have limitations such as low affinity or specificity. Moreover, they are not always well characterized and due to the low abundance of the modification, particularly on mRNAs, generated data sets might resemble noise rather than specific modification patterns. Therefore, it is critical that the affinity and specificity is rigorously tested using complementary approaches. Here, we provide an experimental toolbox that allows for testing antibody performance prior to their use. |
| تدمد: | 1469-9001 1355-8382 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::774ba88d110b3d78cd7df016f58b05c2Test https://doi.org/10.1261/rna.076026.120Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....774ba88d110b3d78cd7df016f58b05c2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14699001 13558382 |
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