Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by Streptomyces sp. 8812
| العنوان: | Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by Streptomyces sp. 8812 |
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| المؤلفون: | Aleksandra Rajnisz, Magdalena Postek, Adam Guśpiel, Jolanta Solecka, Anna Laskowska, Daniel Rabczenko, Joanna Ziemska |
| المصدر: | Scopus-Elsevier Polish Journal of Microbiology, Vol 65, Iss 1 (2016) ResearcherID |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Microbiology (medical), lcsh:QH426-470, Sequence analysis, Nitrogen, 030106 microbiology, biologically active compounds, lcsh:QR1-502, media optimization, 01 natural sciences, Applied Microbiology and Biotechnology, Microbiology, Streptomyces, lcsh:Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Biosynthesis, 010608 biotechnology, RNA, Ribosomal, 16S, Enzyme Inhibitors, Mycelium, Phylogeny, chemistry.chemical_classification, Bacteriological Techniques, biology, Chemistry, submerged cultures, General Medicine, Gene Expression Regulation, Bacterial, Ribosomal RNA, biology.organism_classification, 16S ribosomal RNA, Carbon, lcsh:Genetics, RNA, Bacterial, Enzyme, Biochemistry, Streptomyces sp. 8812, Streptomyces capoamus |
| الوصف: | The nutritional requirements and environmental conditions for a submerged culture of Streptomyces sp. 8812 were determined. Batch and fed-batch Streptomyces sp. 8812 fermentations were conducted to obtain high activity of secondary metabolites. In the study several factors were examined for their influence on the biosynthesis of the active metabolites-7-hydroxy-6-oxo-2,3,4,6-tetrahydroisoquinoline-3-carboxyl acid (C10H9NO4) and N-acetyl-3,4-dihydroxy-l-phenylalanine (C11H13NO5): changes in medium composition, pH of production medium, various growth phases of seed culture, amino acid supplementation and addition of anion exchange resin to the submerged culture. Biological activities of secondary metabolites were examined with the use of dd-carboxypeptidase 64–575 and horseradish peroxidase. Streptomyces sp. 8812 mycelium was evaluated under fluorescent microscopy and respiratory activity of the strain was analyzed. Moreover, the enzymatic profiles of the strain with the use of Api®ZYM test were analyzed and genetic analysis made. Phylogenetic analysis of Streptomyces sp. 8812 revealed that its closest relative is Streptomyces capoamus JCM 4734 (98%), whereas sequence analysis for 16S rRNA gene using NCBI BLAST algorithm showed 100% homology between these two strains. Biosynthetic processes, mycelium growth and enzyme inhibitory activities of these two strains were also compared. |
| تدمد: | 1733-1331 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dff15912ac663860f0a1ccdbb40bbd2bTest https://pubmed.ncbi.nlm.nih.gov/27281994Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dff15912ac663860f0a1ccdbb40bbd2b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17331331 |
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