دورية أكاديمية
Discovery of candidate serum proteomic and metabolomic biomarkers in ankylosing spondylitis
| العنوان: | Discovery of candidate serum proteomic and metabolomic biomarkers in ankylosing spondylitis |
|---|---|
| المؤلفون: | Fischer, Roman, Trudgian, David, Wright, Cynthia, Thomas, Gethin, Bradbury, Linda, Brown, Matthew, Bowness, Paul, Kessler, Benedikt |
| المصدر: | Molecular and Cellular Proteomics |
| بيانات النشر: | American Society for Biochemistry and Molecular Biology Inc. |
| سنة النشر: | 2012 |
| المجموعة: | Queensland University of Technology: QUT ePrints |
| مصطلحات موضوعية: | 23 peroxylactone, 25 hydroxycolecalciferol 26, adult, amyloid P component, ankylosing spondylitis, article, biological marker, clinical article, colecalciferol, controlled study, correlation analysis, down regulation, female, human, inter alpha trypsin inhibitor heavy chain 1, liquid chromatography, male, mass spectrometry, metabolite, metabolomics, priority journal, protein blood level, proteomics, quantitative analysis, receiver operating characteristic, trypsin, unclassified drug, upregulation, vitamin D binding protein |
| الوصف: | Ankylosing Spondylitis (AS) is a common inflammatory rheumatic disease with a predilection for the axial skeleton, affecting 0.2% of the population. Current diagnostic criteria rely on a composite of clinical and radiological changes, with a mean time to diagnosis of 5 to 10 years. In this study we employed nano liquid-chromatography mass spectrometry analysis to detect and quantify proteins and small compounds including endogenous peptides and metabolites in serum from 18 AS patients and nine healthy individuals. We identified a total of 316 proteins in serum, of which 22 showed significant up- or down-regulation (p < 0.05) in AS patients. Receiver operating characteristic analysis of combined levels of serum amyloid P component and inter-α-trypsin inhibitor heavy chain 1 revealed high diagnostic value for Ankylosing Spondylitis (area under the curve = 0.98). We also depleted individual sera of proteins to analyze endogenous peptides and metabolic compounds. We detected more than 7000 molecular features in patients and healthy individuals. Quantitative MS analysis revealed compound profiles that correlate with the clinical assessment of disease activity. One molecular feature identified as a Vitamin D3 metabolite-(23S,25R)-25-hydroxyvitamin D3 26,23-peroxylactone-was down-regulated in AS. The ratio of this vitamin D metabolite versus vitamin D binding protein serum levels was also altered in AS as compared with controls. These changes may contribute to pathological skeletal changes in AS. Our study is the first example of an integration of proteomic and metabolomic techniques to find new biomarker candidates for the diagnosis of Ankylosing Spondylitis |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://eprints.qut.edu.au/89342/1/89342.pdfTest; Fischer, Roman, Trudgian, David, Wright, Cynthia, Thomas, Gethin, Bradbury, Linda, Brown, Matthew, Bowness, Paul, & Kessler, Benedikt (2012) Discovery of candidate serum proteomic and metabolomic biomarkers in ankylosing spondylitis. Molecular and Cellular Proteomics, 11(2), Article number: M111.013904 1-11.; https://eprints.qut.edu.au/89342Test/; Faculty of Health; Institute of Health and Biomedical Innovation |
| الإتاحة: | https://doi.org/10.1074/mcp.M111.013904Test https://eprints.qut.edu.au/89342Test/ |
| حقوق: | free_to_read ; Consult author(s) regarding copyright matters ; This work is covered by copyright. Unless the document is being made available under a Creative Commons Licence, you must assume that re-use is limited to personal use and that permission from the copyright owner must be obtained for all other uses. If the document is available under a Creative Commons License (or other specified license) then refer to the Licence for details of permitted re-use. It is a condition of access that users recognise and abide by the legal requirements associated with these rights. If you believe that this work infringes copyright please provide details by email to qut.copyright@qut.edu.au |
| رقم الانضمام: | edsbas.82D9E671 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |