Opti-nQL: An Optimized, Versatile and Sensitive Nano-LC Method for MS-Based Lipidomics Analysis
| العنوان: | Opti-nQL: An Optimized, Versatile and Sensitive Nano-LC Method for MS-Based Lipidomics Analysis |
|---|---|
| المؤلفون: | Umberto Restuccia, Angela Bachi, Michele Bianchi, Laura Tronci, Angela Cattaneo, Giuseppe Martano, Vittoria Matafora |
| المصدر: | Metabolites, Vol 11, Iss 720, p 720 (2021) Metabolites Volume 11 Issue 11 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Chromatography, quantitative analysis, Chemistry, Endocrinology, Diabetes and Metabolism, Nano lc ms ms, Ms analysis, Cellular lipid, sensitivity, Biochemistry, Microbiology, Article, nano-LC-MS/MS, QR1-502, Highly sensitive, Lipid extraction, High complexity, Lipidomics, lipidomics, lipid species, Molecular Biology, Quantitative analysis (chemistry) |
| الوصف: | Lipidomics is the comprehensive analysis of lipids in a given biological system. This investigation is often limited by the low amount and high complexity of biological samples, therefore highly sensitive lipidomics methods are required. Nanoflow-LC/MS offers extremely high sensitivity however, it is challenging as a more demanding maintenance is often needed compared to conventional microflow-LC approaches. Here, we developed a sensitive and reproducible lipidomics LC method, termed Opti-nQL, which can be applied to any biological system. Opti-nQL has been validated with cellular lipid extracts of human and mouse origin and with different lipid extraction methods. Among the resulting 4000 detected features, 700 and even more unique lipid molecular species have been identified covering 16 lipid sub-classes, while 400 lipids were uniquely structure defined by MS/MS. These results were obtained by analyzing an amount of lipids extract equivalent to 40 ng of proteins, being highly suitable for low abundant samples. MS analysis showed that theOpti-nQL method increases the number of identified lipids, which is evidenced by injecting 20 times less material than in microflow based chromatography, being more reproducible and accurate thus enhancing robustness of lipidomics analysis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2218-1989 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c09b39ea8256e17601d6bb38ec8c23b7Test https://www.mdpi.com/2218-1989/11/11/720Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c09b39ea8256e17601d6bb38ec8c23b7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22181989 |
|---|