التفاصيل البيبلوغرافية
| العنوان: |
Transgene expression enhancement in T-lymphoma cell lines |
| المؤلفون: |
Ruybal, Paula1, Gravisaco, María José1, Barcala, Virna2, Escalada, Ana3, Cremaschi, Graciela4, Taboga, Oscar5, Waldner, Claudia1, Mongini, Claudia1 claudia.mongini@fibertel.com.ar |
| المصدر: |
International Immunopharmacology. Nov2005, Vol. 5 Issue 12, p1685-1692. 8p. |
| مصطلحات موضوعية: |
*GENES, *GENE transfection, *T cells, *LYMPHOMAS, *CYTOMETRY, *GENETIC transformation |
| مستخلص: |
Abstract: In transfection protocols, the expression levels of the transgene is important to define, still is difficult to obtain in certain cell lines such as those derived from T-lymphoma cells. In this study we evaluate transgene expression kinetics in the presence and absence of two well known transcription activators such as phorbol-12-myristate13-acetate (PMA) and Ionomicin (IO). Three murine T lymphoma cell lines (LBC, EL4 and BW5147) were transfected by electroporation using green fluorescent protein (GFP) as a reporter gene and analyzed by flow cytometry. Addition of PMA/IO resulted in a significant increase of the Mean Fluorescence Intensity but not in GFP-positive cell percentages, either in transient or stable transfected LBC and EL4 cells. Remarkable, BW5147 cells showed low GFP induction with a significant increment only in stable transfected cells. Our results demonstrated that CMV promoter activity can be enhanced in transfected lymphoma cells by PMA/IO suggesting that transgene expression levels can be optimized by means of the use of transcription activators. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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