Reliability of cancer cell elasticity in force microscopy
| العنوان: | Reliability of cancer cell elasticity in force microscopy |
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| المؤلفون: | Christian Dietz, Anahid Amiri, Florian D. Hastert, Lars-Oliver Heim |
| المصدر: | Applied Physics Letters. 116:083701 |
| بيانات النشر: | AIP Publishing, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 010302 applied physics, Materials science, Physics and Astronomy (miscellaneous), Cell, Force spectroscopy, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, Contact mechanics, medicine.anatomical_structure, 0103 physical sciences, Cancer cell, Microscopy, medicine, Cancer cell lines, Elasticity (economics), 0210 nano-technology, Elastic modulus, Biomedical engineering |
| الوصف: | Evaluating the mechanical properties of biological cells in their living state is of upmost importance for understanding cellular behavior in health or disease and in particular cancer cells. In recent years, force microscopy and spectroscopy have become inevitable tools to attain the elastic modulus of cells as a whole using colloidal probes or with high lateral precision by sharp tips. However, the obtained values have to be treated with due care to avoid data misinterpretation. Here, we present a comparison of elasticity between four metastatic cancer cell lines (lung, liver, skin, and breast epithelial-type cancer cells) taken by colloidal and sharp probes using force spectroscopy on cell monolayers and stress the impact of a stiff substrate, which cells are attached to, on the measured values especially in cancerous cells, which depict abnormalities in structure and cellular components. As a result of our analysis, we suggest to solely use sharp probes for the mechanical characterization of cancer cells when the force-distance relation is fit by a conventional contact mechanics model such as the Hertz model. |
| تدمد: | 1077-3118 0003-6951 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::fdfe98f7a25b3cb1cc0f39ad6427dca2Test https://doi.org/10.1063/1.5143432Test |
| رقم الانضمام: | edsair.doi...........fdfe98f7a25b3cb1cc0f39ad6427dca2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10773118 00036951 |
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