Localization and quantification of extracellular polymers in methanogenic granular sludge
| العنوان: | Localization and quantification of extracellular polymers in methanogenic granular sludge |
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| المؤلفون: | M. Smit, Alfons J. M. Stams, A. A. M. van Lammeren, Alexander J. B. Zehnder, J.T.C. Grotenhuis |
| المصدر: | Applied Microbiology and Biotechnology 36 (1991) Applied Microbiology and Biotechnology, 36, 115-119 |
| بيانات النشر: | Springer Science and Business Media LLC, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | Laboratorium voor Plantencelbiologie, chemistry.chemical_classification, Chromatography, Extraction (chemistry), General Medicine, Polymer, engineering.material, Polysaccharide, Microbiology, Applied Microbiology and Biotechnology, Laboratory of Plant Cell Biology, Activated sludge, Biochemistry, chemistry, Microbiologie, engineering, Extracellular, Bioreactor, Propionate, Life Science, Biopolymer, Biotechnology |
| الوصف: | Extracellular polymers were localized and quantitatively analysed in methanogenic granular sludge cultivated on either propionate or ethanol in laboratory upflow anaerobic sludge-blanket (UASB) reactors. Electron microscopical analysis of ultrathin sections of the two sludge types stained with ruthenium red revealed the presence of extracellular polymers with different densities and structures. For quantification, granular sludge from a large-scale UASB reactor at a liquid sugar plant was also included in this study. A three-step physical disintegration procedure was used to extract water-soluble extracellular material from the granules. After each disintegration step the extracts were analysed for polysaccharides and proteins. Cell damage and thus the contribution of intracellular proteins and polysaccharides was estimated simultaneously by the determination of free DNA and free ATP in the extracts. After two extraction steps, up to 3.5 mg polysaccharides/g organic material and 5.5 mg protein/g organic material were extracted, whereas no significant increase in DNA was detected. The role of extracellular polymers in granular stability is discussed. |
| وصف الملف: | text/html; application/octet-stream |
| تدمد: | 1432-0614 0175-7598 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d0263629c02fd8856fc212e4decfe10Test https://doi.org/10.1007/bf00164710Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....2d0263629c02fd8856fc212e4decfe10 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320614 01757598 |
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