دورية
Recombinant occlusion bodies of baculovirus as carriers of a non-structural protein of foot-and-mouth disease virus
| العنوان: | Recombinant occlusion bodies of baculovirus as carriers of a non-structural protein of foot-and-mouth disease virus |
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| المؤلفون: | Diez, Michay, Trotta, Myrian, Alfonso, Victoria, Taboga, Oscar, López, María |
| المصدر: | 3 Biotech; November 2018, Vol. 8 Issue: 11 p1-6, 6p |
| مستخلص: | Here, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB1–3, a polypeptide derived from non-structural proteins of foot-and-mouth disease virus, to polyhedrin (POLH), the major constituent of OBs, under polhpromoter. To further assess the most convenient strategy to improve yields, we designed two recombinant baculoviruses, vPOLH and vPOLHE44G. These carried the sequence of the fusion protein POLH-Δ3AB1–3with an additional copy in cis of polhor polhE44G, respectively, under p10promoter. Our results show that both viruses expressed POLH-Δ3AB1–3, which was detected by western blot in purified rOBs with anti-POLH and anti-3AB1 antibodies. We also found that vPOLHE44Gproduced larger polyhedra and a significant increase of antigen yield (p< 0.01). Furthermore, the chimeric protein POLH-Δ3AB1–3was recognized by sera from experimentally infected animals, showing that translational fusion to POLH does not alter the antigenicity of Δ3AB1–3. Finally, the rOBs were successfully used in an ELISA test to differentiate infected from vaccinated animals. Taken together, these results demonstrate the great potential of rOBs to develop diagnostic schemes adaptable to animal infectious diseases. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 2190572X 21905738 |
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| DOI: | 10.1007/s13205-018-1482-x |