D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants
| العنوان: | D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants |
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| المؤلفون: | Wienstroer, J., Engqvist, Martin, 1983, Kunz, H.H., Flügge, U.I., Maurino, V.G. |
| المصدر: | FEBS Letters. 586(1):36-40 |
| مصطلحات موضوعية: | d-Lactate dehydrogenase, Arabidopsis thaliana, Plant transformation, Selection marker |
| الوصف: | d-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media d-lactate concentrations greater than 5-10 mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous d-lactate dehydrogenase (d-LDH) detoxify d-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, d-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on d-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired. |
| الوصول الحر: | https://research.chalmers.se/publication/248107Test http://dx.doi.org/10.1016/j.febslet.2011.11.020Test |
| قاعدة البيانات: | SwePub |
| تدمد: | 18733468 00145793 |
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| DOI: | 10.1016/j.febslet.2011.11.020 |