دورية
Understanding Mucor circinelloidespathogenesis by comparative genomics and phenotypical studies
| العنوان: | Understanding Mucor circinelloidespathogenesis by comparative genomics and phenotypical studies |
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| المؤلفون: | López-Fernández, Loida, Sanchis, Marta, Navarro-Rodríguez, Patricia, Nicolás, Francisco E., Silva-Franco, Fátima, Guarro, Josep, Garre, Victoriano, Navarro-Mendoza, María Isabel, Pérez-Arques, Carlos, Capilla, Javier |
| المصدر: | Virulence; December 2018, Vol. 9 Issue: 1 p707-720, 14p |
| مستخلص: | ABSTRACTThe increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. This study aims to decipher the genetic basis of Mucor circinelloidespathogenicity, which displays virulence in a strain dependent manner. Assuming that genetic differences between strains may be linked to different pathotypes, we have conducted a study to explore genes responsible for virulence in M. circinelloidesby whole genome sequencing of the avirulent strain NRRL3631 and comparison with the virulent strain CBS277.49. This genome analysis revealed 773 truncated, discontiguous and absent genes in the NRRL3631 strain. We also examined phenotypic traits resulting in reduced heat stress tolerance, chitosan content and lower susceptibility to toxic compounds (calcofluor white and sodium dodecyl sulphate) in the virulent strain, suggesting the influence of cell wall on pathogenesis. Based on these results, we focused on studying extracellular protein-coding genes by gene deletion and further pathotype characterization of mutants in murine models of pulmonary and systemic infection. Deletion of gene ID112092, which codes for a hypothetical extracellular protein of unknown function, resulted in significant reduction of virulence.Although pathogenesis is a multifactorial process, these findings highlight the crucial role of surface and secreted proteins in M. circinelloidesvirulence and should promote further studies of other differential genes. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 21505594 21505608 |
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| DOI: | 10.1080/21505594.2018.1435249 |