Cryopreservation of Human Adipose-Derived Stem Cells for Use in Ex Vivo Regional Gene Therapy for Bone Repair
العنوان: | Cryopreservation of Human Adipose-Derived Stem Cells for Use in Ex Vivo Regional Gene Therapy for Bone Repair |
---|---|
المؤلفون: | Osamu Sugiyama, Robert Yoho, Amy H. Tang, Sofia Bougioukli, Venus Vakhshori, Jay R. Lieberman |
المصدر: | Human Gene Therapy Methods. 29:269-277 |
بيانات النشر: | Mary Ann Liebert Inc, 2018. |
سنة النشر: | 2018 |
مصطلحات موضوعية: | 0301 basic medicine, Bone Regeneration, Bone Morphogenetic Protein 2, Applied Microbiology and Biotechnology, Cryopreservation, Viral vector, Andrology, 03 medical and health sciences, Antigens, CD, Genetics, Humans, CD90, Research Articles, Cells, Cultured, Genetics (clinical), Pharmacology, Osteoblasts, biology, Cluster of differentiation, CD44, Cell Differentiation, Mesenchymal Stem Cells, Genetic Therapy, 030104 developmental biology, Adipose Tissue, Cell culture, biology.protein, Molecular Medicine, Stem cell, Ex vivo |
الوصف: | The development of an ex vivo regional gene therapy clinical pathway using adipose-derived stem cells (ASCs) may require cryopreservation for cell culture, storage, and transport prior to clinical use. ASCs isolated from five donors were transduced with a lentiviral vector containing BMP-2. Three groups were assessed: transduction without cell freezing (group 1), freezing of cells for 3 weeks followed by transduction (group 2), and cell transduction prior to freezing (group 3). Nontransduced cells were used as a control. The cluster of differentiation (CD) marker profiles, cell number, BMP-2 production, and osteogenic potential were measured. The CD marker profile (CD44, CD73, CD90, and CD105) was unchanged after cryopreservation. Cell number was equivalent among cryopreservation protocols in transduced and nontransduced cells. There was a trend toward decreased BMP-2 production in group 3 compared to groups 1 and 2. Osteogenic potential based on Alizarin red concentration was higher in group 2 compared to group 3, with no difference compared to group 1. Freezing ASCs prior to transduction with a lentiviral vector containing BMP-2 has no detrimental effect on cell number, BMP-2 production, osteogenic potential, or immunophenotype. Transduction prior to freezing, however, may limit the BMP-2 production and potential osteogenic differentiation of the ASCs. |
تدمد: | 1946-6544 1946-6536 |
الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d5052e20c574b73c34935e2d38c1cb4Test https://doi.org/10.1089/hgtb.2018.191Test |
حقوق: | OPEN |
رقم الانضمام: | edsair.doi.dedup.....1d5052e20c574b73c34935e2d38c1cb4 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 19466544 19466536 |
---|