دورية أكاديمية
Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from Drosophila suzukii Larvae
| العنوان: | Peptidomics as a Tool to Assess the Cleavage of Wine Haze Proteins by Peptidases from Drosophila suzukii Larvae |
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| المؤلفون: | Albuquerque, Wendell Wagner Campos, Ghezellou, Parviz, Lee, Kwang-Zin, Schneider, Quintus, Gross, Phillip, Kessel, Tobias, Omokungbe, Bodunrin, Spengler, Bernhard, Vilcinskas, Andreas, Zorn, Holger, Gand, Martin |
| سنة النشر: | 2023 |
| المجموعة: | Publikationsdatenbank der Fraunhofer-Gesellschaft |
| مصطلحات موضوعية: | chitinases, Drosophila suzukii, peptidases, peptidomics, recombinant proteins, thaumatin-like proteins, wine haze |
| الوصف: | Thermolabile grape berry proteins such as thaumatin-like proteins (TLPs) and chitinases (CHIs) promote haze formation in bottled wines if not properly fined. As a natural grapevine pest, the spotted-wing fly Drosophila suzukii is a promising source of peptidases that break down grape berry proteins because the larvae develop and feed inside mature berries. Therefore, we produced recombinant TLP and CHI as model thermolabile wine haze proteins and applied a peptidomics strategy to investigate whether D. suzukii larval peptidases were able to digest them under acidic conditions (pH 3.5), which are typically found in winemaking practices. The activity of the novel peptidases was confirmed by mass spectrometry, and cleavage sites within the wine haze proteins were visualized in 3D protein models. The combination of recombinant haze proteins and peptidomics provides a valuable screening tool to identify optimal peptidases suitable for clarification processes in the winemaking industry. ; 13 ; 3 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | Biomolecules; #PLACEHOLDER_PARENT_METADATA_VALUE#; https://publica.fraunhofer.de/handle/publica/447791Test |
| DOI: | 10.3390/biom13030451 |
| الإتاحة: | https://doi.org/10.3390/biom13030451Test https://publica.fraunhofer.de/handle/publica/447791Test |
| حقوق: | open access |
| رقم الانضمام: | edsbas.36F01141 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/biom13030451 |
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