دورية أكاديمية
Protective effect of human serum amyloid P on CCl4-induced acute liver injury in mice
| العنوان: | Protective effect of human serum amyloid P on CCl4-induced acute liver injury in mice |
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| المؤلفون: | Cong, Min, Zhao, Weihua, Liu, Tianhui, Wang, Ping, Fan, Xu, Zhai, Qingling, Bao, Xiaoli, Zhang, Dong, You, Hong, Kisseleva, Tatiana, Brenner, David A., Jia, Jidong, Zhuang, Hui |
| المساهمون: | Zhuang, H (reprint author), Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, 38 Xueyuan Rd, Beijing 100191, Peoples R China., Zhuang, H (reprint author), Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Ctr Infect Dis, 38 Xueyuan Rd, Beijing 100191, Peoples R China., Jia, JD (reprint author), Capital Med Univ, Beijing Friendship Hosp, Liver Res Ctr, 95 Yong An Rd, Beijing 100050, Peoples R China., Jia, JD (reprint author), Beijing Key Lab Translat Med Liver Cirrhosis, 95 Yong An Rd, Beijing 100050, Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Microbiol, 38 Xueyuan Rd, Beijing 100191, Peoples R China., Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Ctr Infect Dis, 38 Xueyuan Rd, Beijing 100191, Peoples R China., Capital Med Univ, Beijing Friendship Hosp, Liver Res Ctr, 95 Yong An Rd, Beijing 100050, Peoples R China., Beijing Key Lab Translat Med Liver Cirrhosis, 95 Yong An Rd, Beijing 100050, Peoples R China., Natl Clin Res Ctr Digest Dis, 95 Yong An Rd, Beijing 100050, Peoples R China., Univ Calif San Diego, Dept Surg, La Jolla, CA 92093 USA., Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA., Jia, JD (reprint author), Beijing Key Lab Translat Med Liver Cirrhosis, 95 Yong An Rd, Beijing 100050, Peoples R China., Jia, JD (reprint author), Natl Clin Res Ctr Digest Dis, 95 Yong An Rd, Beijing 100050, Peoples R China. |
| المصدر: | SCI |
| بيانات النشر: | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE |
| سنة النشر: | 2017 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | human serum amyloid P, carbon tetrachloride, inflammation, hepatocytes, apoptosis, hepatic stellate cells, INDUCED PULMONARY-FIBROSIS, CARBON-TETRACHLORIDE, AUTOIMMUNE HEPATITIS, FC-RECEPTOR, CIRRHOSIS, THERAPY, FIBROGENESIS, EXPRESSION |
| الوصف: | Human serum amyloid P (hSAP), a member of the pentraxin family, inhibits the activation of fibrocytes in culture and inhibits experimental renal, lung, skin and cardiac fibrosis. As hepatic inflammation is one of the causes of liver fibrosis, in the present study, we investigated the hepatoprotective effects of hSAP against carbon tetrachloride (CCl4)-induced liver injury. Our data indicated that hSAP attenuated hepatic histopathological abnormalities and significantly decreased inflammatory cell infiltration and pro-inflammatory factor expression. Moreover, CCl4-induced apoptosis in the mouse liver was inhibited by hSAP, as measured by terminal-deoxynucleotidyl transferase mediated nick-end labeling (TUNEL) assay and cleaved caspase-3 expression. hSAP significantly restored the expression of B cell lymphoma/leukemia (Bcl)-2 and suppressed the expression of Bcl-2-associated X protein (Bax) in vivo. The number of hepatocytes in early apoptosis stained with Annexin V was significantly reduced by 28-30% in the hSAP treatment group compared with the CCl4 group, and the expression of Bcl-2 was increased, whereas the expression of Bax and cleaved caspase-3 were significantly inhibited in the hSAP pre-treatment group compared with the CCl4 group. hSAP administration also inhibited the migration and activation of hepatic stellate cells (HSCs) in CCl4-injured liver and suppressed the activation of isolated primary HSCs induced by transforming growth factor (TGF)-beta 1 in vitro. Collectively, these findings suggest that hSAP exerts a protective effect againts CCl4-induced hepatic injury by suppressing the inflammatory response and hepatocyte apoptosis, potentially by inhibiting HSC activation. ; National Natural Science Foundation of China [81570542]; Natural Science Foundation of Beijing Municipality [7142043]; Beijing Health System Talents Plan [2013-3-057] ; SCI(E) ; ARTICLE ; 2 ; 454-464 ; 40 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| تدمد: | 1107-3756 1791-244X |
| العلاقة: | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE.2017,40(2),454-464.; 1905989; http://hdl.handle.net/20.500.11897/471882Test; WOS:000406003700021 |
| DOI: | 10.3892/ijmm.2017.3028 |
| الإتاحة: | https://doi.org/20.500.11897/471882Test https://doi.org/10.3892/ijmm.2017.3028Test https://hdl.handle.net/20.500.11897/471882Test |
| رقم الانضمام: | edsbas.CE294102 |
| قاعدة البيانات: | BASE |
| تدمد: | 11073756 1791244X |
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| DOI: | 10.3892/ijmm.2017.3028 |