دورية أكاديمية
RNA-dependent RNA targeting by CRISPR-Cas9
| العنوان: | RNA-dependent RNA targeting by CRISPR-Cas9 |
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| المؤلفون: | Strutt, Steven C, Torrez, Rachel M, Kaya, Emine, Negrete, Oscar A, Doudna, Jennifer A |
| المساهمون: | National Science Foundation, Howard Hughes Medical Institute, German Academic Exchange Program, Laboratory Directed Research and Development, Paul Allen Frontiers Science Program |
| المصدر: | eLife ; volume 7 ; ISSN 2050-084X |
| بيانات النشر: | eLife Sciences Publications, Ltd |
| سنة النشر: | 2018 |
| المجموعة: | eLife (E-Journal - via CrossRef) |
| الوصف: | Double-stranded DNA (dsDNA) binding and cleavage by Cas9 is a hallmark of type II CRISPR-Cas bacterial adaptive immunity. All known Cas9 enzymes are thought to recognize DNA exclusively as a natural substrate, providing protection against DNA phage and plasmids. Here, we show that Cas9 enzymes from both subtypes II-A and II-C can recognize and cleave single-stranded RNA (ssRNA) by an RNA-guided mechanism that is independent of a protospacer-adjacent motif (PAM) sequence in the target RNA. RNA-guided RNA cleavage is programmable and site-specific, and we find that this activity can be exploited to reduce infection by single-stranded RNA phage in vivo. We also demonstrate that Cas9 can direct PAM-independent repression of gene expression in bacteria. These results indicate that a subset of Cas9 enzymes have the ability to act on both DNA and RNA target sequences, and suggest the potential for use in programmable RNA targeting applications. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.7554/elife.32724 |
| الإتاحة: | https://doi.org/10.7554/elife.32724Test https://cdn.elifesciences.org/articles/32724/elife-32724-v2.pdfTest https://cdn.elifesciences.org/articles/32724/elife-32724-v2.xmlTest https://elifesciences.org/articles/32724Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ ; http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.1D130BEB |
| قاعدة البيانات: | BASE |
| DOI: | 10.7554/elife.32724 |
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