المؤلفون: Da Hyun Lee, Chung Youl Park, Young Ho Jung, Jun Hyeok Kim, Sung Hyuk Park, Ho Jun Son, Chae Sun Na, Wan Geun Park

المصدر: Horticulturae; Volume 8; Issue 9; Pages: 836

مصطلحات موضوعية: physiological dormancy, Vaccinium oldhamii Miq., gibberellic acid, seed dormancy

جغرافية الموضوع: agris

الوصف: Vaccinium oldhamii Miq. is an edible berry; however, little is known about its seed dormancy-break and germination. Therefore, the aim of this study was to elucidate the seed-dormancy break and germination characteristics of V. oldhamii. The results showed that the length and width of the seeds of V. oldhamii were 2.4588 ± 0.0273 mm and 1.7028 ± 0.0248 mm, respectively, the filled percentage was 49.0 ± 3.0%, and the 1000-seed weight was 0.9453 g. Additionally, water imbibition test showed that the seed weight of V. oldhamii increased by more than 70% after 24 h, indicating the absence of physical dormancy. Embryo length measured at dispersal and just before germination did not differ significantly. There was no significant difference in E:S (Embryo:Seed) ratio in length at the time of seed dispersal (0.6780 ± 0.0258) and before germination (0.7370 ± 0.0469), indicating the absence of morphological dominance. Temperature treatments showed that the germination of the seed was 0, 6.0, 6.0, 20.0, and 0% under 15, 20, 25, 25/15, and 15/6 °C, respectively. However, treatment with 250 ppm of GA3 increased the germination of the seeds to 22.0, 36.0, 32.0, 40.0, and 1.0%, respectively. Additionally, treatment with 500 ppm of GA3 increased the germination to 34.6, 40.0, 40.0, 46.0 %, respectively. These results indicated that the seeds of V. oldhamii exhibited Nondeep physiological dormancy (Nondeep PD). Overall, the findings of this study showed that treatment with 500 ppm of GA3 at 25/15 °C can effectively break the dormancy of V. oldhamii seeds, which could facilitate further research on the species.

وصف الملف: application/pdf

العلاقة: Propagation and Seeds; https://dx.doi.org/10.3390/horticulturae8090836Test

الإتاحة: https://doi.org/10.3390/horticulturae8090836Test

المؤلفون: Ha Na Kim, Jueng Kyu Baek, Su Bin Park, Jeong Dong Kim, Ho-Jun Son, Gwang Hun Park, Hyun Ji Eo, Jae Ho Park, Hyuk-Sang Jung, Jin Boo Jeong

المصدر: BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-14 (2019)

مصطلحات موضوعية: Anti-inflammation, Anti-osteoclastogenesis, Inflammatory diseases, Vaccinium oldhamii, Other systems of medicine, RZ201-999

الوصف: Abstract Background Vaccinium oldhamii (V. oldhamii) has been reported to exert a variety of the pharmacological properties such as anti-oxidant activity, anti-cancer activity, and inhibitory activity of α-amylase and acetylcholinesterase. However, the anti-inflammatory activity of V. oldhamii has not been studied. In this study, we aimed to investigate anti-inflammatory activity of the stem extracts from V. oldhamii, and to elucidate the potential mechanisms in LPS-stimulated RAW264.7 cells. Methods Cell viability was evaluated by MTT assay. The determination of NO and PGE2 production was performed using Griess reagent and Prostaglandin E2 ELISA Kit, respectively. The change of mRNA or protein level was evaluated by RT-PCR and Western blot. Results Among VOS, VOL and VOF, the inhibitory effect of NO and PGE2 production induced by LPS was highest in VOS treatment. Thus, VOS was selected for the further study. VOS dose-dependently blocked LPS-induced NO and PGE2 production by inhibiting iNOS and COX-2 expression, respectively. VOS inhibited the expression of pro-inflammatory cytokines such as IL-1β, IL-6 and TNF-α. In addition, VOS suppressed TRAP activity and attenuated the expression of the osteoclast-specific genes such as NFATc1, c-FOS, TRAP, MMP-9, cathepsin K, CA2, OSCAR and ATPv06d2. VOS inhibited LPS-induced NF-κB signaling activation through blocking IκB-α degradation and p65 nuclear accumulation. VOS inhibited MAPK signaling activation by attenuating the phosphorylation of ERK1/2, p38 and JNK. Furthermore, VOS inhibited ATF2 phosphorylation and blocked ATF2 nuclear accumulation. Conclusions These results indicate that VOS may exert anti-inflammatory activity by inhibiting NF-κB and MAPK/ATF2 signaling. From these findings, VOS has potential to be a candidate for the development of chemopreventive or therapeutic agents for the inflammatory diseases.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12906-019-2720-4Test; https://doaj.org/toc/1472-6882Test

الوصول الحر: https://doaj.org/article/cf98aaee2ba1481184720b35ad1559b0Test

المؤلفون: Sang-Chul Kim, Seung-Hoon Baek, Jei-Wan Lee, Hwa Ja Hyun

المصدر: Mitochondrial DNA. Part B. Resources, Vol 4, Iss 1, Pp 902-903 (2019)

مصطلحات موضوعية: vaccinium oldhamii, phylogenetic analysis, plastid genome, ericaceae, Genetics, QH426-470

الوصف: The complete genome of Vaccinium oldhamii plastid was sequenced and analyzed in this study. The V. oldhamii plastid a circular genome of 173,245 bp with 33 genes, including 88 protein, 37 tRNA, and eight rRNA genes. Phylogenetic analysis showed that species of Vaccinium were the first branching taxon In Ericaceae.

العلاقة: http://dx.doi.org/10.1080/23802359.2019.1579067Test; https://doaj.org/toc/2380-2359Test; https://doaj.org/article/3150e1b2a90f494c8ce94e592daf3c85Test

الإتاحة: https://doi.org/10.1080/23802359.2019.1579067Test
https://doaj.org/article/3150e1b2a90f494c8ce94e592daf3c85Test

المؤلفون: Hyun Ji Eo, Gwang Hun Park, Su Bin Park, Jin Boo Jeong, Ho-Jun Son, Jueng Kyu Baek, Jeong Dong Kim, Jae Ho Park, Ha Na Kim, Hyuk-Sang Jung

المصدر: BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-14 (2019)
BMC Complementary and Alternative Medicine

مصطلحات موضوعية: Lipopolysaccharides, MAPK/ERK pathway, Anti-osteoclastogenesis, p38 mitogen-activated protein kinases, Anti-Inflammatory Agents, Inflammatory diseases, Dinoprostone, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Western blot, Anti-inflammation, medicine, Animals, Humans, MTT assay, Viability assay, Prostaglandin E2, 030304 developmental biology, Inflammation, 0303 health sciences, Activating Transcription Factor 2, Plant Stems, medicine.diagnostic_test, Vaccinium oldhamii, Tumor Necrosis Factor-alpha, Chemistry, Macrophages, NF-kappa B, NF-κB, General Medicine, lcsh:Other systems of medicine, lcsh:RZ201-999, Molecular biology, RAW 264.7 Cells, Complementary and alternative medicine, Cyclooxygenase 2, 030220 oncology & carcinogenesis, Phosphorylation, Mitogen-Activated Protein Kinases, Vaccinium, Research Article, medicine.drug

الوصف: Background Vaccinium oldhamii (V. oldhamii) has been reported to exert a variety of the pharmacological properties such as anti-oxidant activity, anti-cancer activity, and inhibitory activity of α-amylase and acetylcholinesterase. However, the anti-inflammatory activity of V. oldhamii has not been studied. In this study, we aimed to investigate anti-inflammatory activity of the stem extracts from V. oldhamii, and to elucidate the potential mechanisms in LPS-stimulated RAW264.7 cells. Methods Cell viability was evaluated by MTT assay. The determination of NO and PGE2 production was performed using Griess reagent and Prostaglandin E2 ELISA Kit, respectively. The change of mRNA or protein level was evaluated by RT-PCR and Western blot. Results Among VOS, VOL and VOF, the inhibitory effect of NO and PGE2 production induced by LPS was highest in VOS treatment. Thus, VOS was selected for the further study. VOS dose-dependently blocked LPS-induced NO and PGE2 production by inhibiting iNOS and COX-2 expression, respectively. VOS inhibited the expression of pro-inflammatory cytokines such as IL-1β, IL-6 and TNF-α. In addition, VOS suppressed TRAP activity and attenuated the expression of the osteoclast-specific genes such as NFATc1, c-FOS, TRAP, MMP-9, cathepsin K, CA2, OSCAR and ATPv06d2. VOS inhibited LPS-induced NF-κB signaling activation through blocking IκB-α degradation and p65 nuclear accumulation. VOS inhibited MAPK signaling activation by attenuating the phosphorylation of ERK1/2, p38 and JNK. Furthermore, VOS inhibited ATF2 phosphorylation and blocked ATF2 nuclear accumulation. Conclusions These results indicate that VOS may exert anti-inflammatory activity by inhibiting NF-κB and MAPK/ATF2 signaling. From these findings, VOS has potential to be a candidate for the development of chemopreventive or therapeutic agents for the inflammatory diseases.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf813e681121b869829241ff806fff45Test
http://link.springer.com/article/10.1186/s12906-019-2720-4Test

المؤلفون: Dai Hirose, Fraidoon Karimi, Naoaki Watanabe, Nobumitsu Sasaki, Nobuo Kobayashi, Takashi Baba, Takuya Ban, Yuji Kurashige

المصدر: Microbes and Environments. 2016, 31(2):186

المؤلفون: Byeong-Gil Son, Ji-Suk Jeong, Geun-Bae Go, Su-Jin Park, Yeon-Kwon Jung

المصدر: Korean Journal of Food & Cookery Science. 32:316-324

مصطلحات موضوعية: 0301 basic medicine, 03 medical and health sciences, 030109 nutrition & dietetics, Traditional medicine, Chemistry, Corni Fructus, Browning, General Earth and Planetary Sciences, Vaccinium oldhamii, Quality characteristics, General Environmental Science

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::d557e25515835341c219920afdc75e7aTest
https://doi.org/10.9724/kfcs.2016.32.3.316Test

المؤلفون: KyeongWon Yun, Jeong Ho Song, Lee Uk, Moon Seob Kim

المصدر: Journal of Korean Forest Society. 105:208-215

مصطلحات موضوعية: 0301 basic medicine, chemistry.chemical_classification, 030109 nutrition & dietetics, Antioxidant, medicine.medical_treatment, Flavonoid, Vaccinium oldhamii, 03 medical and health sciences, chemistry.chemical_compound, chemistry, Polyphenol, Anthocyanin, medicine, Food science

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::65d3b356b9cedb2edd5c0b1beb60d22dTest
https://doi.org/10.14578/jkfs.2016.105.2.208Test

المؤلفون: Nobuo Kobayashi, Dai Hirose, Takuya Ban, Fraidoon Karimi, Nobumitsu Sasaki, Takashi Baba, Naoaki Watanabe, Yuji Kurashige

المصدر: Microbes and Environments. 31:186-189

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, biology, Biodiversity, Soil Science, Plant Science, General Medicine, Vaccinium oldhamii, Dark septate endophyte, biology.organism_classification, 01 natural sciences, Plant use of endophytic fungi in defense, 03 medical and health sciences, 030104 developmental biology, Hair root, Mycorrhizal fungi, Botany, Colonization, Ecology, Evolution, Behavior and Systematics, 010606 plant biology & botany, Vaccinium

الوصف: The root diameters as well as colonization and diversity of the root-associating fungi of Vaccinium oldhamii Miq. were investigated in order to obtain information on their mycorrhizal properties. The distal regions of roots had typical hair roots with diameters of less than 100 μm. Ericoid mycorrhizal fungi (ErMF) and dark septate endophytes (DSE) were frequently observed in the roots. Ascomycetes, particularly helotialean fungi, appeared to be dominant among the endophytic fungi of V. oldhamii roots. Furthermore, Rhizoscyphus ericae (Read) Zhuang & Korf and Oidiodendron maius Barron known as ErMF were detected more frequently than other fungal species.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::696ee66bf6c0b71c8de07a3f96c05d44Test
https://doi.org/10.1264/jsme2.me16011Test

المؤلفون: Hwa Ja Hyun, Jei-Wan Lee, Sang-Chul Kim, Seunghoon Baek

المصدر: Mitochondrial DNA Part B. 4:902-903

مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Genetics, Phylogenetic tree, biology, fungi, food and beverages, Vaccinium oldhamii, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, Genome, Chloroplast, 03 medical and health sciences, 030104 developmental biology, Ericaceae, Transfer RNA, Plastid, Molecular Biology, Gene

الوصف: The complete genome of Vaccinium oldhamii plastid was sequenced and analyzed in this study. The V. oldhamii plastid a circular genome of 173,245 bp with 33 genes, including 88 protein, 37 tRNA, and...

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::b9685377dec6eb49c058bf656dc0ee8bTest
https://doi.org/10.1080/23802359.2019.1579067Test

المؤلفون: 정은주 ( Eun Ju Cheong )

المصدر: Journal of Korean Forest Society. 104:193-197

مصطلحات موضوعية: chemistry.chemical_compound, chemistry, Anthocyanin, Botany, Vaccinium oldhamii, Biology

الوصف: 국내 자생 정금나무의 기능성 품종을 육성하기 위하여 4집단 24개체에 대한 열매의 형태적 특성과 안토시아닌 함량을 분석하였다. 분산분석결과 조사된 모든 형질 특성들에서 집단간 및 집단내 개체간에 유의적인 차이가인정되었다. 정금나무 열매의 평균특성을 살펴보면 열매종경 8.2 mm, 열매횡경 7.8 mm, 열매무게 0.4 g, 열매당도11.7 brix., 과지당 열매수 7.7개, 안토시아닌 함량 474.4 mg/100 g으로 각각 나타났다. 특히, 정금나무의 열매특성은미국산 블루베리에 비해 크기는 작으나 안토시아닌 함량이 1.9배 높은 것으로 나타나 기능성 품종육성을 위한 소재로서의 이용 가치가 높은 수종으로 판단된다. 기능성 품종육성을 위하여 3가지(열매무게, 과지당 열매수, 안토시아닌함량) 주요 선발기준에 의해 최종 선발된 3개체의 선발효과를 추정한 결과 전체 평균특성과 비교하여 열매무게129.6%(0.36 g→0.47 g), 과지당 열매수 109.5%(7.7개→8.4개), 안토시아닌 함량 140.5%(474.4 mg/100 g→666.4 mg/ 100 g)의 선발효과를 추정할 수 있었다.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::adcb6f98acb18909134ea7ddfc5b9775Test
https://doi.org/10.14578/jkfs.2015.104.2.193Test