المؤلفون: Masato Shimada, Yoshihisa Koyama, Yuki Kobayashi, Yasunari Matsumoto, Hikaru Kobayashi, Shoichi Shimada

المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract The progression of small bowel ischemia–reperfusion (IR) injury causes cells in the intestinal tract to undergo necrosis, necessitating surgical resection, which may result in loss of intestinal function. Therefore, developing therapeutic agents that can prevent IR injury at early stages and suppress its progression is imperative. As IR injury may be closely related to oxidative stress, antioxidants can be effective therapeutic agents. Our silicon (Si)-based agent, an antioxidant, generated a large amount of hydrogen in the intestinal tract for a prolonged period after oral administration. As it has been effective for ulcerative colitis, renal failure, and IR injury during skin flap transplantation, it could be effective for small intestinal IR injury. Herein, we investigated the efficacy of an Si-based agent in a mouse model of small intestinal IR injury. The Si-based agent suppressed the apoptosis of small intestinal epithelial cells by reducing the oxidative stress induced by IR injury. In addition, the thickness of the mucosal layer in the small intestine of the Si-based agent-administered group was significantly higher than that in the untreated group, revealing that Si-based agent is effective against small intestinal IR injuries. In the future, Si-based agents may improve the success rate of small intestine transplantation.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/56e6f774a5754df2a7bb051a88a7fc37Test

المؤلفون: Tomoo Yuba, Yoshihisa Koyama, Chiyo Ootaki, Yuji Fujino, Shoichi Shimada

المصدر: Heliyon, Vol 10, Iss 14, Pp e34573- (2024)

مصطلحات موضوعية: Oxidative stress, D-ROMs, BAP, Storage period, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: The Diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests can easily and rapidly measure the state of oxidative stress in the blood; they have been used to determine the relationship between oxidative stress and various diseases. However, the extent to which the blood storage period affects the analyzed data remains unclear. In clinical practice, the storage conditions for samples after blood collection vary. Therefore, the influence of blood storage conditions, particularly the reversible redox state, on biochemical tests has been thoroughly investigated. The storage conditions of the sample may affect its state; however, its effect on oxidative stress has not been investigated yet. In this study, considering that the time from blood collection to blood cell separation differs depending on the clinical setting, we analyzed the effect of storage period on the redox analysis data of blood samples stored for a certain period in a 4 °C refrigerator without centrifugation. Heparinized plasma samples from three healthy adult men in their 30s were subjected to the d-ROMs and BAP tests. The analysis was performed at the following 12 time points: immediately after blood collection; 1, 3, 6, 12, and 24 h later; and 2, 3, 4, 5, 6, and 7 days later. The d-ROMs and BAP values varied and were unstable after 1 h of blood collection. These findings suggest that centrifugation should be performed within 1 h after blood collection, at the latest. In a clinical setting, data should be interpreted with caution if centrifugation is performed more than 1 h after blood collection, even if heparin is added and the samples are stored at 4 °C.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844024106044Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/edbe64516e7042ea92d9fdbf3072e61dTest

المؤلفون: Yuki Kinishi, Yoshihisa Koyama, Tomoo Yuba, Yuji Fujino, Shoichi Shimada

المصدر: Frontiers in Neuroscience, Vol 18 (2024)

مصطلحات موضوعية: epidural-related maternal fever, intrapartum fever, epidural anesthesia, interleukin-1 receptor antagonist, sterile inflammation, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

الوصف: Fever during childbirth, which is often observed in clinical settings, is characterized by a temperature of 38°C or higher, and can occur due to infectious and non-infectious causes. A significant proportion of non-infectious causes are associated with epidural-related maternal fever during vaginal delivery. Therapeutic interventions are required because fever has adverse effects on both mother and newborn. Effective treatment options for ERMF are lacking. As it is difficult to distinguish it from intrauterine infections such as chorioamnionitis, antibiotic administration remains the only viable option. We mentioned the importance of interleukin-1 receptor antagonist in the sterile inflammatory fever pathway and the hormonal influence on temperature regulation during childbirth, an important factor in elucidating the pathophysiology of ERMF. This review spotlighted the etiology and management of ERMF, underscoring recent advancements in our understanding of hypothalamic involvement in thermoregulation and its link to sterile inflammation. We propose to deepen the understanding of ERMF within the broader context of autonomic neuroscience, aiming to foster the development of targeted therapies.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fnins.2024.1389132/fullTest; https://doaj.org/toc/1662-453XTest

الوصول الحر: https://doaj.org/article/174313c84afb471a953ba76c7a637258Test

المؤلفون: Masato Shimada, Yoshihisa Koyama, Yuki Kobayashi, Hikaru Kobayashi, Shoichi Shimada

المصدر: Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Interstitial pneumonia (IP) is a collective term for diseases whose main lesion is fibrosis of the pulmonary interstitium, and the prognosis associated with acute exacerbation of these conditions is often poor. Therapeutic agents are limited to steroids, immunosuppressants, and antifibrotic drugs, which and have many side effects; therefore, the development of new therapeutic agents is required. Because oxidative stress contributes to lung fibrosis in IP, optimal antioxidants may be effective for the treatment of IP. Silicon (Si)-based agents, when administered orally, can continuously generate a large amount of antioxidant hydrogen in the intestinal tract. In this study, we investigated the effect of our Si-based agent on methotrexate-induced IP, using the IP mouse models. Pathological analysis revealed that interstitial hypertrophy was more significantly alleviated in the Si-based agent-treated group than in the untreated group (decreased by about 22%; P

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/27cb769d78a549deb3218d717931a026Test

المؤلفون: Ikuei Hirota, Yoshihisa Koyama, Shoichi Shimada

المصدر: Biochemistry and Biophysics Reports, Vol 34, Iss , Pp 101467- (2023)

مصطلحات موضوعية: Formalin test, Biphasic property, Spinal neuronal activity, Capsaicin, c-Fos, Biology (General), QH301-705.5, Biochemistry, QD415-436

الوصف: The formalin test has been established as a method for evaluating mouse models of pain. Although there have been numerous reports of formalin-pain-induced behavior, few reports of a detailed histochemical analysis of the central nervous system focus on behavioral biphasic properties.To investigate the alternation of spinal neuronal activity with formalin-induced pain, we performed immunofluorescent staining with c-Fos antibodies as neuronal activity markers using acute pain model mice induced by 2% formalin stimulation. As a result, phase-specific expression patterns were observed. In the spinal dorsal horn region, there were many neural activities in the deep region (layers V−VII) in the behavioral first phase and those in the surface region (layers I−III) in the behavioral second phase. Furthermore, we conducted comparative studies using low concentrations (0.25%) of formalin and capsaicin, which did not show distinct behavioral biphasic properties. Neural activity was observed only in the spinal dorsal horn surface region for both stimuli.Our study suggested that the histochemical biphasic nature of formalin-induced pain was attributable to the activity of the deep region of the spinal cord. In the future, treatment strategies focusing on the deep region neuron will lead to the development of effective treatments for allodynia and intractable chronic pain.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405580823000481Test; https://doaj.org/toc/2405-5808Test

الوصول الحر: https://doaj.org/article/12407b29c37049d6a667f233c56ef99aTest

المؤلفون: Akira Yamamoto, Harumi Gomi, Hideki Takami, Hiroyuki Komatsu, Hitoaki Okazaki, Hitoshi Hasegawa, Keiichiro Suzuki, Masanaga Yamawaki, Masato Eto, Masatomi Ikusaka, Osamu Nomura, Shoichi Shimada, Takeshi Onoue, Yasuhiko Konishi, Yasushi Matsuyama, 小松 弘幸, 小西 靖彦, 尾上 剛史, 山本 憲, 山脇 正永, 岡崎 仁昭, 島田 昌一, 松山 泰, 江頭 正人, 生坂 政臣, 矢野 晴美, 野村 理, 鈴木 敬一郎, 長谷川 仁志, 高見 秀樹

المصدر: 医学教育 / Igaku Kyoiku / Medical Education (Japan). 2023, 54(2):157

المؤلفون: Daizo Koinuma, Keiichiro Suzuki, Kumiko Yamaguchi, Masanaga Yamawaki, Shoichi Shimada, Takeo Nakayama, 中山 健夫, 山口 久美子, 山脇 正永, 島田 昌一, 鈴木 敬一郎, 鯉沼 代造

المصدر: 医学教育 / Igaku Kyoiku / Medical Education (Japan). 2023, 54(2):171

المؤلفون: Yoshihisa Koyama, Yuki Kobayashi, Ikuei Hirota, Yuanjie Sun, Iwao Ohtsu, Hiroe Imai, Yoshichika Yoshioka, Hiroto Yanagawa, Takuya Sumi, Hikaru Kobayashi, Shoichi Shimada

المصدر: Scientific Reports, Vol 12, Iss 1, Pp 1-17 (2022)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Ulcerative colitis (UC) is a non-specific inflammatory bowel disease that causes ulcers and erosions in the colonic mucosa and becomes chronic with cycles of amelioration and exacerbation. Because its exact etiology remains largely unclear, and the primary therapy is limited to symptomatic treatment, the development of new therapeutic agent for UC is highly desired. Because one of the disease pathogenesis is involvement of oxidative stress, it is likely that an appropriate antioxidant will be an effective therapeutic agent for UC. Our silicon (Si)-based agent, when ingested, allowed for stable and persistent generation of massive amounts of hydrogen in the gastrointestinal tract. We demonstrated the Si-based agent alleviated the mental symptom as well as the gastrointestinal symptoms, inflammation, and oxidation associated with dextran sodium sulfate-induced UC model through Hydrogen and antioxidant sulfur compounds. As the Si-based agent was effective in treating UC in the brain and large intestine of mice, it was considered to be capable of suppressing exacerbations and sustaining remission of UC.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/6ee01ba95c7442478f2d139954a5a160Test

المؤلفون: Satoshi Deyama, Makoto Kondo, Shoichi Shimada, Katsuyuki Kaneda

المصدر: Translational Psychiatry, Vol 12, Iss 1, Pp 1-10 (2022)

مصطلحات موضوعية: Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

الوصف: Abstract Ketamine, an N-methyl-D-aspartate receptor antagonist, exerts rapid and sustained antidepressant actions. Preclinical studies demonstrated that the release of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor in the medial prefrontal cortex (mPFC) is essential for the antidepressant-like effects of ketamine. However, the role of other neurotrophic factors in the antidepressant-like effects of ketamine has not been fully investigated. Since the intra-mPFC infusion of insulin-like growth factor 1 (IGF-1) reportedly produced antidepressant-like effects, the present study examined the role of endogenous intra-mPFC IGF-1 signaling in the antidepressant-like actions of ketamine. In vivo microdialysis showed that ketamine (10 and 30 mg/kg) significantly increased extracellular IGF-1 levels in the mPFC of male C57BL/6J mice for at least 5 h. Infusion of an IGF-1 neutralizing antibody (nAb; 160 ng/side) into the mPFC 15 min before or 2 h after ketamine injection blocked the antidepressant-like effects of ketamine in three different behavioral paradigms (forced swim, female urine sniffing, and novelty-suppressed feeding tests were conducted 1, 3 and 4 days post-ketamine, respectively). The ketamine-like antidepressant-like actions of the intra-mPFC infusion of BDNF (100 ng/side) and IGF-1 (50 ng/side) respectively were not blocked by co-infused IGF-1 nAb and BDNF nAb (200 ng/side). Moreover, intra-mPFC infusion of IGF-1 nAb 2 h post-ketamine blocked the antidepressant-like effects of ketamine in a murine lipopolysaccharide (LPS)-induced depression model. Intra-mPFC IGF-1 infusion also produced antidepressant-like effects in the LPS-challenged mice via mechanistic target of rapamycin complex 1 activation. These results suggest that persistent release of IGF-1, independently of BDNF, in the mPFC is essential for the antidepressant-like actions of ketamine.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2158-3188Test

الوصول الحر: https://doaj.org/article/0a03b7d4562f4e3589cf27f2360d4690Test

المؤلفون: Yoshihisa Koyama, Yuki Kobayashi, Ikuei Hirota, Yuanjie Sun, Iwao Ohtsu, Hiroe Imai, Yoshichika Yoshioka, Hiroto Yanagawa, Takuya Sumi, Hikaru Kobayashi, Shoichi Shimada

المصدر: Scientific Reports, Vol 12, Iss 1, Pp 1-3 (2022)

مصطلحات موضوعية: Medicine, Science

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/3413d16680db48898ac3b607468dbb72Test