المؤلفون: Natale G., Pignataro A., Marino G., Campanelli F., Calabrese V., Cardinale A., Pelucchi S., Marcello E., Gardoni F., Viscomi M. T., Picconi B., Ammassari-Teule M., Calabresi P., Ghiglieri V.

المساهمون: G. Natale, A. Pignataro, G. Marino, F. Campanelli, V. Calabrese, A. Cardinale, S. Pelucchi, E. Marcello, F. Gardoni, M.T. Viscomi, B. Picconi, M. Ammassari-Teule, P. Calabresi, V. Ghiglieri

مصطلحات موضوعية: dendritic spine, GluN2B, noninvasive brain stimulation, partial dopamine denervation, striatum, Settore BIO/14 - Farmacologia

الوصف: Background: In experimental models of Parkinson's disease (PD), different degrees of degeneration to the nigrostriatal pathway produce distinct profiles of synaptic alterations that depend on progressive changes in N-methyl-D-aspartate receptors (NMDAR)-mediated functions. Repetitive transcranial magnetic stimulation (rTMS) induces modifications in glutamatergic and dopaminergic systems, suggesting that it may have an impact on glutamatergic synapses modulated by dopamine neurotransmission. However, no studies have so far explored the mechanisms of rTMS effects at early stages of PD. Objectives: We tested the hypothesis that in vivo application of rTMS with intermittent theta-burst stimulation (iTBS) pattern alleviates corticostriatal dysfunctions by modulating NMDAR-dependent plasticity in a rat model of early parkinsonism. Methods: Dorsolateral striatal spiny projection neurons (SPNs) activity was studied through ex vivo whole-cell patch-clamp recordings in corticostriatal slices obtained from 6-hydroxydopamine-lesioned rats, subjected to a single session (acute) of iTBS and tested for forelimb akinesia with the stepping test. Immunohistochemical analyses were performed to analyze morphological correlates of plasticity in SPNs. Results: Acute iTBS ameliorated limb akinesia and rescued corticostriatal long-term potentiation (LTP) in SPNs of partially lesioned rats. This effect was abolished by applying a selective inhibitor of GluN2B-subunit-containing NMDAR, suggesting that iTBS treatment could be associated with an enhanced activation of specific NMDAR subunits, which are major regulators of structural plasticity during synapse development. Morphological analyses of SPNs revealed that iTBS treatment reverted dendritic spine loss inducing a prevalence of thin-elongated spines in the biocytin-filled SPNs. Conclusions: Taken together, our data identify that an acute iTBS treatment produces a series of plastic changes underlying striatal compensatory adaptation in the parkinsonian basal ganglia circuit.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/34339069; info:eu-repo/semantics/altIdentifier/wos/WOS:000680070300001; volume:36; issue:10; firstpage:2254; lastpage:2263; numberofpages:10; journal:MOVEMENT DISORDERS; http://hdl.handle.net/2434/867452Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85111833132

الإتاحة: https://doi.org/10.1002/mds.28671Test
http://hdl.handle.net/2434/867452Test

المؤلفون: Campanelli F., Viscomi M. T., Calabresi P.

المساهمون: Natale, G., Pignataro, A., Marino, G., Campanelli, Federica, Calabrese, V., Cardinale, A., Pelucchi, S., Marcello, E., Gardoni, F., Viscomi, Maria Teresa, Picconi, B., Ammassari-Teule, M., Calabresi, Paolo, Ghiglieri, V.

مصطلحات موضوعية: dendritic spines, GluN2B, noninvasive brain stimulation, partial dopamine denervation, striatum, Animals, Corpus Striatum, Neuronal Plasticity, Rats, Synapses, Dopamine, Transcranial Magnetic Stimulation, Settore BIO/17 - ISTOLOGIA

الوصف: Background: In experimental models of Parkinson's disease (PD), different degrees of degeneration to the nigrostriatal pathway produce distinct profiles of synaptic alterations that depend on progressive changes in N-methyl-D-aspartate receptors (NMDAR)-mediated functions. Repetitive transcranial magnetic stimulation (rTMS) induces modifications in glutamatergic and dopaminergic systems, suggesting that it may have an impact on glutamatergic synapses modulated by dopamine neurotransmission. However, no studies have so far explored the mechanisms of rTMS effects at early stages of PD. Objectives: We tested the hypothesis that in vivo application of rTMS with intermittent theta-burst stimulation (iTBS) pattern alleviates corticostriatal dysfunctions by modulating NMDAR-dependent plasticity in a rat model of early parkinsonism. Methods: Dorsolateral striatal spiny projection neurons (SPNs) activity was studied through ex vivo whole-cell patch-clamp recordings in corticostriatal slices obtained from 6-hydroxydopamine-lesioned rats, subjected to a single session (acute) of iTBS and tested for forelimb akinesia with the stepping test. Immunohistochemical analyses were performed to analyze morphological correlates of plasticity in SPNs. Results: Acute iTBS ameliorated limb akinesia and rescued corticostriatal long-term potentiation (LTP) in SPNs of partially lesioned rats. This effect was abolished by applying a selective inhibitor of GluN2B-subunit-containing NMDAR, suggesting that iTBS treatment could be associated with an enhanced activation of specific NMDAR subunits, which are major regulators of structural plasticity during synapse development. Morphological analyses of SPNs revealed that iTBS treatment reverted dendritic spine loss inducing a prevalence of thin-elongated spines in the biocytin-filled SPNs. Conclusions: Taken together, our data identify that an acute iTBS treatment produces a series of plastic changes underlying striatal compensatory adaptation in the parkinsonian basal ganglia circuit. © 2021 ...

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/34339069; info:eu-repo/semantics/altIdentifier/wos/WOS:000680070300001; volume:36; issue:10; firstpage:2254; lastpage:2263; numberofpages:10; issueyear:2021; journal:MOVEMENT DISORDERS; http://hdl.handle.net/10807/187381Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85111833132

الإتاحة: https://doi.org/10.1002/mds.28671Test
http://hdl.handle.net/10807/187381Test

المؤلفون: Giuseppina Natale, Antonella Cardinale, Federica Campanelli, Silvia Pelucchi, Veronica Ghiglieri, Maria Teresa Viscomi, Barbara Picconi, Annabella Pignataro, Paolo Calabresi, Fabrizio Gardoni, Valeria Calabrese, Martine Ammassari-Teule, Gioia Marino, Elena Marcello

مصطلحات موضوعية: Dendritic spine, medicine.medical_treatment, striatum, Dopamine, Nigrostriatal pathway, Striatum, Medium spiny neuron, Neuroplasticity, medicine, Animals, noninvasive brain stimulation, Neuronal Plasticity, business.industry, Dopaminergic, Long-term potentiation, dendritic spines, Transcranial Magnetic Stimulation, Corpus Striatum, Rats, Transcranial magnetic stimulation, GluN2B, medicine.anatomical_structure, Neurology, Synapses, Neurology (clinical), Settore BIO/17 - ISTOLOGIA, business, Neuroscience, partial dopamine denervation

الوصف: Background In experimental models of Parkinson's disease (PD), different degrees of degeneration to the nigrostriatal pathway produce distinct profiles of synaptic alterations that depend on progressive changes in N-methyl-D-aspartate receptors (NMDAR)-mediated functions. Repetitive transcranial magnetic stimulation (rTMS) induces modifications in glutamatergic and dopaminergic systems, suggesting that it may have an impact on glutamatergic synapses modulated by dopamine neurotransmission. However, no studies have so far explored the mechanisms of rTMS effects at early stages of PD. Objectives We tested the hypothesis that in vivo application of rTMS with intermittent theta-burst stimulation (iTBS) pattern alleviates corticostriatal dysfunctions by modulating NMDAR-dependent plasticity in a rat model of early parkinsonism. Methods Dorsolateral striatal spiny projection neurons (SPNs) activity was studied through ex vivo whole-cell patch-clamp recordings in corticostriatal slices obtained from 6-hydroxydopamine-lesioned rats, subjected to a single session (acute) of iTBS and tested for forelimb akinesia with the stepping test. Immunohistochemical analyses were performed to analyze morphological correlates of plasticity in SPNs. Results Acute iTBS ameliorated limb akinesia and rescued corticostriatal long-term potentiation (LTP) in SPNs of partially lesioned rats. This effect was abolished by applying a selective inhibitor of GluN2B-subunit-containing NMDAR, suggesting that iTBS treatment could be associated with an enhanced activation of specific NMDAR subunits, which are major regulators of structural plasticity during synapse development. Morphological analyses of SPNs revealed that iTBS treatment reverted dendritic spine loss inducing a prevalence of thin-elongated spines in the biocytin-filled SPNs. Conclusions Taken together, our data identify that an acute iTBS treatment produces a series of plastic changes underlying striatal compensatory adaptation in the parkinsonian basal ganglia circuit. © 2021 The Authors. Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19982c1af6996d0c73030f2bdb41f0a1Test
http://hdl.handle.net/10807/187381Test

مستخلص: Background: In experimental models of Parkinson's disease (PD), different degrees of degeneration to the nigrostriatal pathway produce distinct profiles of synaptic alterations that depend on progressive changes in N-methyl-D-aspartate receptors (NMDAR)-mediated functions. Repetitive transcranial magnetic stimulation (rTMS) induces modifications in glutamatergic and dopaminergic systems, suggesting that it may have an impact on glutamatergic synapses modulated by dopamine neurotransmission. However, no studies have so far explored the mechanisms of rTMS effects at early stages of PD. Objectives: We tested the hypothesis that in vivo application of rTMS with intermittent theta-burst stimulation (iTBS) pattern alleviates corticostriatal dysfunctions by modulating NMDAR-dependent plasticity in a rat model of early parkinsonism. Methods: Dorsolateral striatal spiny projection neurons (SPNs) activity was studied through ex vivo whole-cell patch-clamp recordings in corticostriatal slices obtained from 6-hydroxydopamine-lesioned rats, subjected to a single session (acute) of iTBS and tested for forelimb akinesia with the stepping test. Immunohistochemical analyses were performed to analyze morphological correlates of plasticity in SPNs. Results: Acute iTBS ameliorated limb akinesia and rescued corticostriatal long-term potentiation (LTP) in SPNs of partially lesioned rats. This effect was abolished by applying a selective inhibitor of GluN2B-subunit-containing NMDAR, suggesting that iTBS treatment could be associated with an enhanced activation of specific NMDAR subunits, which are major regulators of structural plasticity during synapse development. Morphological analyses of SPNs revealed that iTBS treatment reverted dendritic spine loss inducing a prevalence of thin-elongated spines in the biocytin-filled SPNs. Conclusions: Taken together, our data identify that an acute iTBS treatment produces a series of plastic changes underlying striatal compensatory adaptation in th

مصطلحات الفهرس: dendritic spines, GluN2B, noninvasive brain stimulation, partial dopamine denervation, striatum, Animals, Corpus Striatum, Neuronal Plasticity, Rats, Synapses, Dopamine, Transcranial Magnetic Stimulation, Settore BIO/17 - ISTOLOGIA, info:eu-repo/semantics/article

URL: http://hdl.handle.net/10807/187381Test
info:eu-repo/semantics/altIdentifier/pmid/34339069
info:eu-repo/semantics/altIdentifier/wos/WOS:000680070300001
volume:36
issue:10
firstpage:2254
lastpage:2263
numberofpages:10
issueyear:2021
journal:MOVEMENT DISORDERS