المؤلفون: Ahmed A. A. Aioub, Sameh A. Abdelnour, Ahmed S. Hashem, Mohamed Maher, Sarah I. Z. Abdel-Wahab, Lamya Ahmed Alkeridis, Mustafa Shukry, Samy M. Sayed, Ahmed E. A. Elsobki

المصدر: BMC Veterinary Research, Vol 20, Iss 1, Pp 1-18 (2024)

مصطلحات موضوعية: Acetamiprid, Cinnamon nanoemulsions, Biochemical changes, Histological and immunobiological analysis, In silico analysis, Veterinary medicine, SF600-1100

الوصف: Abstract Acetamiprid (ACDP) is a widely used neonicotinoid insecticide that is popular for its efficacy in controlling fleas in domestic settings and for pets. Our study aims to offer a comprehensive examination of the toxicological impacts of ACDP and the prophylactic effects of cinnamon nanoemulsions (CMNEs) on the pathological, immunohistochemical, and hematological analyses induced by taking ACDP twice a ‎week for 28 days. Forty healthy rats were divided into four groups (n = 10) at random; the first group served as control rats; the second received CMNEs (2 mg/Kg body weight); the third group received acetamiprid (ACDP group; 21.7 mg/Kg body weight), and the fourth group was given both ACDP and CMNEs by oral gavage. Following the study period, tissue and blood samples were extracted and prepared for analysis. According to a GC-MS analysis, CMNEs had several bioactive ingredients that protected the liver from oxidative stress by upregulating antioxidant and anti-inflammatory agents. Our findings demonstrated that whereas ACDP treatment considerably boosted white blood cells (WBCs) and lymphocytes, it significantly lowered body weight gain (BWG), red blood cells (RBCs), hemoglobin (Hb), hematocrit (HCT), and platelets (PLT). ACDP notably reduced antioxidant enzyme activities: superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) and elevated hydrogen peroxide and malondialdehyde levels compared with other groups. ACDP remarkably raised alanine aminotransferase (ALT), aspartate amino transaminase (AST), and alkaline phosphatase (ALP) levels. Moreover, the histopathological and immunohistochemistry assays discovered a severe toxic effect on the liver and kidney following ACDP delivery. Furthermore, cyclooxygenase 2 (COX-2) + immunoexpression was enhanced after treatment with CMNEs. All of the parameters above were returned to nearly normal levels by the coadministration of CMNEs. The molecular docking of cinnamaldehyde with COX-2 also confirmed the protective potential of CMNEs against ACDP toxicity. Our findings highlighted that the coadministration of CMNEs along with ACDP diminished its toxicity by cutting down oxidative stress and enhancing antioxidant capacity, demonstrating the effectiveness of CMNEs in lessening ACDP toxicity.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1746-6148Test

الوصول الحر: https://doaj.org/article/e0bd4df8e44b42f5a982f196a867b548Test

المؤلفون: Salvia Trujillo, Laura

المساهمون: University/Department: Universitat de Lleida. Departament de Tecnologia d'Aliments

مرشدي الرسالة: Martín Belloso, Olga, Soliva-Fortuny, Robert

المصدر: TDX (Tesis Doctorals en Xarxa)

مصطلحات موضوعية: Nanoemulsions, Olis essencials, Carotè, Nanoemulsiones, Aceites esenciales, Caroteno, Essential oils, Carotene, Tecnologia d'Aliments

الوصف: Les nanoemulsions es consideren una excel·lent manera d’incorporar compostos lipòfils actius en aliments degut a les seves propietats específiques. L’objectiu d’aquesta tesi fou estudiar els factors que afecten les propietats fisicoquímiques i de funcionalitat de les nanoemulsions com a portadores d’antimicrobians (olis essencials) o compostos bioactius (β-carotè). D’una banda, el mètode de fabricació i els paràmetres de processat, així com la concentració dels seus components i el tipus d’oli essencial van afectar significativament la mida de partícula de les nanoemulsions. Així, es van obtenir nanoemulsions amb una mida de partícula inferior als 10 nm, les quals van presentar una major activitat antimicrobiana que les emulsions convencionals. D’altra banda, les nanoemulsions van presentar una major y més ràpida digestibilitat in vitro de l’oli que les emulsions convencionals, i per tant, una major bioaccessibilitat del β-carotè. La present investigació representa un important avenç per a l’ús de les nanoemulsions en aliments.

الوصف (مترجم): Las nanoemulsiones se consideran una excelente manera de incorporar ingredientes lipófilos activos en los alimentos debido a sus propiedades específicas. El objetivo de esta tesis fue estudiar los factores que afectan las propiedades físico-químicas y de funcionalidad de nanoemulsiones conteniendo antimicrobianos (aceites esenciales) o compuestos bioactivos (β- caroteno). Por un lado, el método de fabricación y los parámetros de procesado así como la concentración de los distintos componentes y el tipo de aceite esencial afectaron significativamente el tamaño de partícula de las nanoemulsiones. Así, se obtuvieron nanoemulsiones con un tamaño de partícula inferior a los 10 nm, presentando una mayor actividad antimicrobiana en comparación con las emulsiones convencionales. Por otro lado, las nanoemulsiones mostraron una mayor y más rápida digestibilidad in vitro del aceite que las emulsiones convencionales, y por tanto, una mayor bioacessibilidad del β-caroteno. La presente investigación supone un importante avance para el uso de nanoemulsiones en los alimentos.
Nanoemulsions are currently regarded as an excellent tool to incorporate active lipophilic ingredients in foods systems due to their properties. The aim of this thesis was to unravel the factors affecting the physicochemical characteristics and the functionality of nanoemulsions containing antimicrobials (essential oils) or bioactive compounds (β-carotene). On one hand, the fabrication method and the processing parameters as well as the concentration of the different components and the essential oil type significantly influenced the droplet size of nanoemulsions. In this sense, nanoemulsions with a droplet size below 10 nm were obtained, which presented an enhanced antimicrobial activity compared to conventional emulsions with larger droplet size. On the other hand, nanoemulsions exhibited a faster and greater oil digestibility than conventional emulsions under simulated digestion conditions and, in turn, a higher β-carotene bioaccessibility. The research carried out reveals important information for the use of nanoemulsions in foods.

وصف الملف: application/pdf

الوصول الحر: http://hdl.handle.net/10803/134733Test

المؤلفون: Bo Tang, Qiuxiang Wang, Guowei Zhang, Aiwen Zhang, Lu Zhu, Rongrong Zhao, Hongwei Gu, Jie Meng, Junfang Zhang, Guihua Fang

المصدر: Journal of Nanobiotechnology, Vol 22, Iss 1, Pp 1-19 (2024)

مصطلحات موضوعية: Stearoyl l-carnitine, Nanoemulsions, Novel organic cation/carnitine transporter 2 (OCTN2), Amino acid transporter B (0 +) (ATB0,+), Endotoxin-induced uveitis, Ocular drug bioavailability, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5

الوصف: Abstract Traditional eye drops are administered via topical instillation. However, frequent dosing is needed due to their relatively rapid precorneal removal and low ocular bioavailability. To address these issues, stearoyl L-carnitine-modified nanoemulsions (SC-NEs) were fabricated. The physicochemical properties of SC-NEs in terms of size, morphology, zeta potential, encapsulation efficiency, and in vitro drug release behavior were characterized. The cellular uptake and mechanisms of SC-NEs were comprehensively studied in human corneal epithelial cells and the stearoyl L-carnitine ratio in SC-NEs was optimized. The optimized SC-NEs could target the novel organic cation/carnitine transporter 2 (OCTN2) and amino acid transporter B (0 +) (ATB0,+) on the corneal epithelium, which led to superior corneal permeation, ocular surface retention ability, ocular bioavailability. Furthermore, SC-NEs showed excellent in vivo anti-inflammatory efficacy in a rabbit model of endotoxin-induced uveitis. The ocular safety test indicated that the SC-NEs were biocompatible. In general, the current study demonstrated that OCTN2 and ATB0,+-targeted nanoemulsions were promising ophthalmologic drug delivery systems that can improve ocular drug bioavailability and boost the therapeutic effects of drugs for eye diseases. Graphical Abstract

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1477-3155Test

الوصول الحر: https://doaj.org/article/4dea0e7eccf142e791bc6ecc25879de1Test

المؤلفون: Fernández Campos, Francisco

المساهمون: University/Department: Universitat de Barcelona. Departament de Farmacologia i Química Terapèutica

مرشدي الرسالة: Calpena Campmany, Ana Cristina, Clares Naveros, Beatriz

المصدر: TDX (Tesis Doctorals en Xarxa)

مصطلحات موضوعية: Emulsions (Farmàcia), Emulsiones (Farmacia), Emulsions (Pharmacy), Nanoemulsions, Nanoemulsiones, Nistatina, Candidiasi, Candidiasis, Malalties de la pell, Enfermedades de la piel, Skin diseases, Membrana mucosa, Mucous membrane, Ciències de la Salut

الوصف: Se han desarrollado y optimizado dos nanoemulsiones que contienen Nistatina (N1 y N2) para su aplicación en piel y mucosa oral, respectivamente. Estas formulaciones tuvieron un tamaño de gota nanométrico y resultaron ser estables a lo largo del tiempo. Adicionalmente se determinó el comportamiento reológico de ambos sistemas resultando ser fluidos newtonianos. Se evaluó la actividad antifúngica in vitro, observado que la potencia de la nistatina aumentó al ser introducida en las nanoemulsiones, cuando se comparaba con la nistatina libre. El mecanismo de liberación de la nistatina de las nanoemulsiones siguió una cinética de orden uno para ambas formulaciones. Trás el ensayo de permeación ex vivo en piel humana con la nanoemulsion N1 se observó que la cantidad de fármaco permeada fue muy baja, pudiendo descartarse la posible aparición de efectos adversos a nivel sistémico. De la misma manera se realizó el ensayo de permeación ex vivo en mucosa oral porcina para la formulación N2 llegando a la misma conclusión que en el caso de la permeación en piel. En ambos casos la cantidad de fármaco retenido en el tejido (tanto en piel o mucosa) es suficiente para observar un efecto fungistático, fungicida y un prolongado efecto post-antifungico (PAFE). Con el fin de determinar el efecto de la nanoemulsion N2 sobre la mucosa oral se visualizó la ultra-estructura del tejido y no observándose variaciones significativas cuando se comparaban con el control (mucosa no tratada con la nanoemulsion). Las nanoemulsiones N1 y N2 son formulaciones prometedoras para el potencial tratamiento clínico de candidiasis muco-cutáneas.

الوصف (مترجم): Muco-cutaneous candidosis is a common opportunistic infection must be treated to prevent other tissue invasion. Nystatin is one of the most prescribed drugs to treat this pathology, but due to its physicochemical properties its pharmaceutical-technological requirements make it a challenge. The purpose of this work was the development and characterization of an optimal nystatin delivery system for the potential treatment of oral candidosis avoiding undesirable side effects and toxicity of potential systemic absorption. Two nanoemulsion (N1 and N2) was developed, evaluated and characterized. It has been formulated successfully as a stable nanoemulsion with a droplet size of 75 and 138 nm, respectively. First order release parameters were estimated using different mathematical approaches and ex vivo permeation of nystatin through human skin and porcine buccal mucosa were found no systemic effects would happen. Microbiologic studies performed revealed an enhanced antifungal effect of the nystatin loaded nanoemulsion. Also the evaluation of buccal mucosa ultrastructure by transmission electron microscopy methodology showing a harmless effect in the mucosa microstructure. We can infer that the selected nystatin nanoemulsion could be potentially used on candidosis infection under mucositis conditions.

وصف الملف: application/pdf

الوصول الحر: http://hdl.handle.net/10803/83638Test

المؤلفون: Konstantinos Zaharioudakis, Constantinos E. Salmas, Nikolaos D. Andritsos, Eleni Kollia, Areti Leontiou, Vasillios K. Karabagias, Andreas Karydis-Messinis, Dimitrios Moschovas, Nikolaos E. Zafeiropoulos, Apostolos Avgeropoulos, Charalampos Proestos, Aris E. Giannakas

المصدر: Frontiers in Food Science and Technology, Vol 4 (2024)

مصطلحات موضوعية: caseinate nanoemulsions, active edible coatings, citral, eugenol, carvacrol, cinnamaldehyde, Food processing and manufacture, TP368-456

الوصف: Introduction: Nowadays, the bioeconomy and sustainability trends drive the food sector to replace chemical preservatives with bioactive compounds recovered from biomass such as essential oils (EOs) and their derivatives. Moreover, nanotechnology trend drives food sector in the nanoencapsulation of such bioactive compounds in novel edible formulations such as nanoemulsions to enhance their controlled release properties, their bioactivity and their biocompatibility.Methods: In this study the development and characterization of novel casein/lecithin (CSN/LCN) based nanoemulsions (NEs) with carvacrol (CV), cinnamaldehyde (CI), citral (CT), and eugenol (EG) as nanoencapsulated oil phase is presented as well as the application of such NEs as active coatings for the preservation of fresh pork tenderloin.Results and discussion: DLS measurements as well as TEM images showed an average particle size distribution of 20–40 nm for all obtained NEs, while z-potential results indicate their physicochemical stability. All the obtained NEs needed at least 5 times less amount of EOs derivatives to scavenge 50% of radicals and demonstrated a higher antibacterial activity against Escherichia coli and Listeria monocytogenes compared to free EOs derivatives. Pork fresh tenderloin meat treated with NEs demonstrated 30% higher efficacy in obtained lipid oxidation values than uncoated pork meat samples and 12% higher efficacy in obtained lipid oxidation values than pork meat samples treated with EOs derivatives. From a microbiological point of view NEs manage to extend pork tenderloin meat shelf life by 6 days compared to uncoated pork tenderloin meat and by 3 days compared to tenderloin pork meat coated with free EOs derivatives. Lab* colorimetry analysis as well as sensory analysis revealed that such edible CSN/LCN based NEs succeeded to preserve the texture, appearance, and color of pork tenderloin meat in higher acceptance limits compared to the corresponding characteristics of pork tenderloin meat coated with free EOs derivatives. Overall, the current study suggests novel CSN/LCN-based edible NEs as a promising technology that can be used as edible active coatings in the meat food industry.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/frfst.2024.1400224/fullTest; https://doaj.org/toc/2674-1121Test

الوصول الحر: https://doaj.org/article/38fbd6bfdd334d9da79e153f5920c248Test

المؤلفون: Taiwo Oreoluwa Ajayi, Madan Sai Poka, Bwalya Angel Witika

المصدر: Frontiers in Medical Technology, Vol 6 (2024)

مصطلحات موضوعية: paediatric, tuberculosis, nanoemulsions (NE), drug delivery, preformulation compatibility, solubility studies, Medical technology, R855-855.5

الوصف: Preformulation investigations into the development of drug formulations, encompassing considerations related to the structure of the drug, excipients, composition, and physical attributes are crucial. This phase is pivotal in ensuring the ultimate success of nanoemulsion development. The objective of this study was to evaluate and define the properties of bedaquiline (BDQ) and the necessary excipients for the formulation of self-emulsifying BDQ-loaded nanoemulsions. To determine the saturation solubility of BDQ in various oils, an in-house validated HPLC method was used. Fourier transform infrared spectroscopy was utilised to identify and evaluate the compatibility between BDQ and the selected excipients. The water titration method was used to construct phase diagrams to identify the type of structure that resulted following emulsification and to characterise the behaviour of mixtures along dilution paths. The solubility studies revealed that BDQ exhibited the highest solubility in olive oil, with a solubility of 3.45 ± 0.041 mg/ml. The design space led to the formation of emulsions categorised as Winsor products. Importantly, the FTIR data indicated the absence of any potential interactions between BDQ and the chosen excipients. The preformulation studies were successful and facilitated the selection of compatible and suitable excipients for the formulation of BDQ-loaded nanoemulsions.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fmedt.2024.1388113/fullTest; https://doaj.org/toc/2673-3129Test

الوصول الحر: https://doaj.org/article/2fc3b0bbb21242fba7cd9c478a244c96Test

المؤلفون: Rachna Gupta, Parth Malik, Ruma Rani, Raghu Solanki, Rakesh Kumar Ameta, Vibhuti Malik, Tapan Kumar Mukherjee

المصدر: Plant Nano Biology, Vol 8, Iss , Pp 100073- (2024)

مصطلحات موضوعية: Nanoemulsions, Hydrophilic-lipophilic balances, Physicochemical characterization, Nanopesticides, Sustainable practices, Botany, QK1-989

الوصف: Nature friendly and sustainable practices have been the prominent aspects reviving the modern agricultural practices. Development of broad spectrum insecticides with the minimal use, maximum efficacy and least environmental deterioration are swiftly emerging as reliable measures. Analogous to drug delivery in animal and human cells, nanocarriers are swiftly emerging as biocompatible and nature friendly aids for pesticide delivery to the agricultural crops. These practices manifest a higher importance for the agriculturally intensive global economies, wherein substantial livelihood means are eventually dependent on agriculture. Amicably transcended from the extraordinary investigational success for drug delivery, trafficking of pesticides through nanoemulsions has emerged as a boost to safeguard the environment and aquatic habitats in particular. The nanoemulsions, with the option of varying surfactant and co-surfactants, engineer the slow release of pesticides which could be targeted for the pest specific elimination. The outcomes have already eased the farmer’s economy besides significantly moderating the toxicity. The threat to soil and surrounding water bodies has been the most significant, wherein almost 90% of the unaided pesticide used to run off as excessive chemical load in the soils or water bodies. The constitutional robustness of emulsions with varied hydrophilic-lipophilic balances and surfactant-co-surfactant stoichiometries have been the distinguishing aspect for the pesticide delivery to the crops. With such insights, this review article focuses on emulsification, the distinguishing working principles, physicochemical characterization driven performance control parameters and finally a discussion of past five year attempts encompassing nanocarrier mediated pesticide delivery for sustainable agriculture and reduced environmental stress.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2773111124000160Test; https://doaj.org/toc/2773-1111Test

الوصول الحر: https://doaj.org/article/8bd0f8ed5522475ead5767bb6966ff09Test

المؤلفون: Yotsanan Weerapol, Suwisit Manmuan, Sontaya Limmatvapirat, Chutima Limmatvapirat, Jitnapa Sirirak, Poomipat Tamdee, Sukannika Tubtimsri

المصدر: OpenNano, Vol 17, Iss , Pp 100207- (2024)

مصطلحات موضوعية: Monolaurin, Enveloped virus, Nanoemulsions, Respiratory disease, SARS-CoV-2, Influenza A (H1N1), Therapeutics. Pharmacology, RM1-950

الوصف: Monolaurin was utilized to formulate temperature-driven phase inversion nanoemulsions containing lesser galangal essential oil, fixed oil, and Cremophor RH40, with aim for eradicating enveloped viruses. Results showed that the droplet size of the nanoemulsion depended on lesser galangal essential oil–fixed oil ratio, monolaurin concentration, and oil concentration. Nanoemulsions prepared from lesser galangal essential oil–perilla oil (60:40) exhibited approximately 50-nm nanosized droplets and high entrapment efficiency (98.68 % ± 2.45 %). After storage at 25 °C for 1 year, droplet size did not vary significantly from the initial size, and monolaurin content was >95 %, indicating good physical and chemical stability. The monolaurin was located at the oil–water interface as indicated by a two-dimensional nuclear Overhauser effect nuclear magnetic resonance spectroscope and computer simulation. The 0.2% w/v monolaurin nanoemulsion inhibited SARS-CoV-2 and influenza A (H1N1) viruses with efficacy more than 3 log reduction (99.90 %) and low cytotoxicity. Hence, the monolaurin nanoemulsion can successfully eradicate enveloped viruses, especially SARS-CoV-2 and influenza A (H1N1).

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2352952024000082Test; https://doaj.org/toc/2352-9520Test

الوصول الحر: https://doaj.org/article/a634de7bf3f646088d727d51a076a6f6Test

المؤلفون: Janga, R. B.^{1, 1}, Prasad, Siva Sunkara¹, Lakshmi, Sowjanya Battula¹, Ramu, A.¹

المصدر: Current Trends in Biotechnology and Pharmacy 18(1):1645-1654. 2024

المؤلفون: Jaishankar Prasad, Aishwarya Dixit, Sujata P. Sharma, Anjelina W. Mwakosya, Anka T. Petkoska, Ashutosh Upadhyay, Nishant Kumar

المصدر: Foods and Raw Materials, Vol 12, Iss 1, Pp 22-36 (2024)

مصطلحات موضوعية: nanoemulsions, active packaging, essential oils, synthesis methods, shelf life, edible films, Food processing and manufacture, TP368-456

الوصف: Recently, there has been an increasing trend in the food and pharmaceutical industries towards using nanotechnological approaches to drug delivery and active packaging (edible coatings and films). In the food sector, nanoemulsions are the most promising technology for delivering active components and improving the barrier, mechanical, and biological properties of packaging to ensure the safety and quality of food products, as well as extend their shelf life. For this review, we used several databases (Google Scholar, Science Direct, PubMed, Web of Science, Scopus, Research Gate, etc.) to collect information about nanoemulsions and their role in edible packaging. We searched for articles published between 2015 and 2022 and described different scientific approaches to developing active packaging systems based on nanoemulsions, as well as their high-energy and low-energy synthesis methods. We also reviewed the uses of different types of essential oil-based nanoemulsions in the packaging of food products to prolong their shelf life and ensure safety. Non-migratory active packaging and active-release packaging systems were also discussed, as well as their advantages and disadvantages.

وصف الملف: electronic resource

العلاقة: https://jfrm.ru/en/issues/21683/21675Test/; https://doaj.org/toc/2308-4057Test; https://doaj.org/toc/2310-9599Test

الوصول الحر: https://doaj.org/article/afec444afaf2438e82510193d2433304Test