المؤلفون: Segal, Meirav, Biscans, Annabelle, Gilles, Maud-Emmanuelle, Anastasiadou, Eleni, De Luca, Roberto, Lim, Jihoon, Khvorova, Anastasia, Slack, Frank J

المساهمون: Segal, Meirav, Biscans, Annabelle, Gilles, Maud-Emmanuelle, Anastasiadou, Eleni, De Luca, Roberto, Lim, Jihoon, Khvorova, Anastasia, Slack, Frank J

مصطلحات موضوعية: high mobility group a2 protein, microrna, microrna let 7b, oligonucleotide, unclassified drug

الوصف: MicroRNAs (miRNAs) have increasingly been shown to be involved in human cancer, and interest has grown about the potential use of miRNAs for cancer therapy. miRNA levels are known to be altered in cancer cells, including in non-small cell lung cancer (NSCLC), a subtype of lung cancer that is the most prevalent form of cancer worldwide and that lacks effective therapies. The let-7 miRNA is involved in the regulation of oncogene expression in cells and directly represses cancer growth in the lung. let-7 is therefore a potential molecular target for tumor therapy. However, applications of RNA interference for cancer research have been limited by a lack of simple and efficient methods to deliver oligonucleotides (ONs) to cancer cells. In this study, we have used in vitro and in vivo approaches to show that HCC827 cells internalize hydrophobically modified let-7b miRNAs (hmiRNAs) added directly to the culture medium without the need for lipid formulation. We identified functional let-7b hmiRNAs targeting the HMGA2 mRNA, one of the let-7 target genes upregulated in NSCLC, and show that direct uptake in HCC827 cells induced potent and specific gene silencing in vitro and in vivo. Thus, hmiRNAs constitute a novel class of ONs that enable functional studies of genes involved in cancer biology and are potentially therapeutic molecules.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31855835; info:eu-repo/semantics/altIdentifier/wos/WOS:000519557700024; volume:19; firstpage:267; lastpage:277; numberofpages:11; journal:MOLECULAR THERAPY NUCLEIC ACIDS; https://hdl.handle.net/11573/1677928Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85076362585

الإتاحة: https://doi.org/10.1016/j.omtn.2019.11.008Test
https://hdl.handle.net/11573/1677928Test

المؤلفون: Karoopongse E., Yeung C., Byon J., Ramakrishnan A., Holman Z.J., Jiang P.Y.Z., Yu Q., Deeg H.J., Marcondes A.M.

المساهمون: PHYSIOLOGY

المصدر: Unpaywall 20191101

مصطلحات موضوعية: 3 deazaneplanocin A, azacitidine, cyclin D1, histone demethylase, microRNA, microRNA let 7b, protein KDM2B, protein p16, transcription factor EZH2, unclassified drug, EZH2 protein, human, F box protein, histone, KDM2A protein, mirnlet7 microRNA, polycomb repressive complex 2, Article, bone marrow culture, CD34 selection, cell cycle G0 phase, cell cycle G1 phase, cell cycle S phase, cell proliferation, controlled study, DNA methylation, epigenetics, gene overexpression, gene repression, gene silencing

الوصف: 10.1371/journal.pone.0107817 ; PLoS ONE ; 9 ; 9 ; e107817

العلاقة: Karoopongse E., Yeung C., Byon J., Ramakrishnan A., Holman Z.J., Jiang P.Y.Z., Yu Q., Deeg H.J., Marcondes A.M. (2014). The KDM2B-let-7b-EZH2 axis in myelodysplastic syndromes as a target for combined epigenetic therapy. PLoS ONE 9 (9) : e107817. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0107817Test; https://scholarbank.nus.edu.sg/handle/10635/161772Test

الإتاحة: https://doi.org/10.1371/journal.pone.0107817Test
https://scholarbank.nus.edu.sg/handle/10635/161772Test

المؤلفون: Oztemur, Y., Bekmez, T., Aydos, A., Yulug I.G., Bozkurt, B., Dedeoglu, B.G.

المصدر: PLoS ONE

مصطلحات موضوعية: B lymphocyte induced maturation protein 1, biological marker, dendritic cell lysosome associated membrane protein, heme oxygenase 1, mammalian target of rapamycin, messenger RNA, microRNA, microRNA 30a 3p, microRNA 30a 5p, microRNA 320, microRNA 7, microRNA 99a, microRNA let 7, microRNA let 7a, microRNA let 7b, microRNA let 7c, microRNA let 7d, microRNA let 7e, microRNA let 7f, microRNA let 7g, microRNA let 7i, minichromosome maintenance protein 2, mitogen activated protein kinase, monocarboxylate transporter 4, suppressor of cytokine signaling 1, thioredoxin reductase 1, transforming growth factor beta, unclassified drug, vasculotropin, apoptosis

الوصف: Breast cancer is one of the most important causes of cancer-related deaths worldwide in women. In addition to gene expression studies, the progressing work in the miRNA area including miRNA microarray studies, brings new aspects to the research on the cancer development and progression. Microarray technology has been widely used to find new biomarkers in research and many transcriptomic microarray studies are available in public databases. In this study, the breast cancer miRNA and mRNA microarray studies were collected according to the availability of their data and clinical information, and combined by a newly developed ranking-based meta-analysis approach to find out candidate miRNA biomarkers (meta-miRNAs) that classify breast cancers according to their grades and explain the relation between miRNAs and mRNAs. This approach provided meta-miRNAs specific to breast cancer grades, pointing out let-7 family members as grade classifiers. The qRTPCR studies performed with independent breast tumors confirmed the potential biomarker role of let-7 family members (meta-miRNAs). The concordance between the meta-mRNAs and miRNA target genes specific to tumor grade (common genes) supported the idea of mRNAs as miRNA targets. The pathway analysis results showed that most of the let-7 family miRNA targets, and also common genes, were significantly taking part in cancer-related pathways. The qRT-PCR studies, together with bioinformatic analyses, confirmed the results of meta-analysis approach, which is dynamic and allows combining datasets from different platforms. © 2015 Oztemur et al.

وصف الملف: application/pdf

العلاقة: http://dx.doi.org/10.1371/journal.pone.0126837Test; http://hdl.handle.net/11693/21948Test

الإتاحة: https://doi.org/10.1371/journal.pone.0126837Test
http://hdl.handle.net/11693/21948Test

المؤلفون: Bala Gur Dedeoglu, Isik G. Yulug, Alp Aydos, Tufan Bekmez, Yasemin Öztemur, Betül Bozkurt

المصدر: PLoS ONE
PLoS ONE, Vol 10, Iss 5, p e0126837 (2015)

مصطلحات موضوعية: PRDM1 gene, Microarray, genetic association, TYMS gene, dendritic cell lysosome associated membrane protein, lcsh:Medicine, Bioinformatics, SLC16A3 gene, ME1 gene, Transcriptome, LAMP3 gene, SQSTM1 gene, RDH10 gene, LMNB2 gene, SAMSN1 gene, lcsh:Science, microRNA 30a 3p, Oligonucleotide Array Sequence Analysis, transforming growth factor beta, Multidisciplinary, microRNA, messenger RNA, mitogen activated protein kinase, apoptosis, microRNA 99a, gene expression regulation, minichromosome maintenance protein 2, biological marker, monocarboxylate transporter 4, unclassified drug, Gene Expression Regulation, Neoplastic, SEC24A gene, Meta-analysis, cancer grading, SOCS1 gene, cell cycle, IQGAP3 gene, Female, DNA microarray, down regulation, POLR2C gene, signal transduction, heme oxygenase 1, Research Article, Genetic Markers, PSAT1 gene, Concordance, TXNRD1 gene, microRNA let 7, Breast Neoplasms, Biology, Real-Time Polymerase Chain Reaction, Article, SOAT1 gene, reverse transcription polymerase chain reaction, TRIP13 gene, Breast cancer, breast cancer, medicine, thioredoxin reductase 1, Humans, microRNA 320, Computer Simulation, human, SLC25A13 gene, gene, microRNA 30a 5p, mammalian target of rapamycin, microRNA 7, STMN1 gene, microRNA let 7d, microRNA let 7e, POLA2 gene, microRNA let 7f, microRNA let 7g, B lymphocyte induced maturation protein 1, vasculotropin, microRNA let 7i, lcsh:R, SLC7A5 gene, TCF19 gene, SELL gene, medicine.disease, MCM2 gene, microRNA let 7a, microRNA let 7b, human tissue, microRNA let 7c, HMOX1 gene, MicroRNAs, suppressor of cytokine signaling 1, Gene chip analysis, lcsh:Q, SRM gene, upregulation

الوصف: Breast cancer is one of the most important causes of cancer-related deaths worldwide in women. In addition to gene expression studies, the progressing work in the miRNA area including miRNA microarray studies, brings new aspects to the research on the cancer development and progression. Microarray technology has been widely used to find new biomarkers in research and many transcriptomic microarray studies are available in public databases. In this study, the breast cancer miRNA and mRNA microarray studies were collected according to the availability of their data and clinical information, and combined by a newly developed ranking-based meta-analysis approach to find out candidate miRNA biomarkers (meta-miRNAs) that classify breast cancers according to their grades and explain the relation between miRNAs and mRNAs. This approach provided meta-miRNAs specific to breast cancer grades, pointing out let-7 family members as grade classifiers. The qRTPCR studies performed with independent breast tumors confirmed the potential biomarker role of let-7 family members (meta-miRNAs). The concordance between the meta-mRNAs and miRNA target genes specific to tumor grade (common genes) supported the idea of mRNAs as miRNA targets. The pathway analysis results showed that most of the let-7 family miRNA targets, and also common genes, were significantly taking part in cancer-related pathways. The qRT-PCR studies, together with bioinformatic analyses, confirmed the results of meta-analysis approach, which is dynamic and allows combining datasets from different platforms. © 2015 Oztemur et al.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d9b2e646f82654defa29b32a7b5e6c9Test
http://europepmc.org/articles/PMC4433233Test

المؤلفون: Clancy, Jennifer, Swaminathan, Sanjay, Suzuki, Kazuo, Seddiki, Nabila, Kaplan, Warren, Cowley, Mark J., Hood, Chantelle, Gelgor, Linda, Cooper, David A, Kelleher, Anthony D

المصدر: Journal of Immunology

مصطلحات موضوعية: Keywords: Gag protein, interleukin 10, microRNA, microrna let 7, microRNA let 7b, microRNA let 7c, microRNA let 7d, microRNA let 7e, microRNA let 7f, microRNA let 7g, unclassified drug, 3' untranslated region, adult, article, CD4+ T lymphocyte, cell culture, clinic

الوصف: MicroRNAs (miRNAs) are ∼22-nt small RNAs that are important regulators of mRNA turnover and translation. Recent studies have shown the importance of the miRNA pathway in HIV-1 infection, particularly in maintaining latency. Our initial in vitro studies

العلاقة: http://hdl.handle.net/1885/78137Test; https://openresearch-repository.anu.edu.au/bitstream/1885/78137/5/01_Clancy_Differential_regulation_of_the_2012.pdf.jpgTest

الإتاحة: https://doi.org/10.4049/jimmunol.1101196Test
http://hdl.handle.net/1885/78137Test
https://openresearch-repository.anu.edu.au/bitstream/1885/78137/5/01_Clancy_Differential_regulation_of_the_2012.pdf.jpgTest

المصدر: Journal of Immunology

مستخلص: MicroRNAs (miRNAs) are ∼22-nt small RNAs that are important regulators of mRNA turnover and translation. Recent studies have shown the importance of the miRNA pathway in HIV-1 infection, particularly in maintaining latency. Our initial in vitro studies

مصطلحات الفهرس: Keywords: Gag protein; interleukin 10; microRNA; microrna let 7; microRNA let 7b; microRNA let 7c; microRNA let 7d; microRNA let 7e; microRNA let 7f; microRNA let 7g; unclassified drug; 3' untranslated region; adult; article; CD4+ T lymphocyte; cell culture; clinic, Journal article

URL: http://hdl.handle.net/1885/78137Test