المؤلفون: Köseler, Aylin, Ma, F., Kılıç, Ä°smail DoÄŸu, Morselli, M., Kilic, O., Pellegrini, M.

مصطلحات موضوعية: acyl coenzyme A synthetase medium chain family member 5, AFG3 like matrix AAA peptidase subunit 2, apolipoprotein B, aspartate aminotransferase, Humans, Male, Myocardial Infarction, Phenotype, Prognosis, Promoter Regions, Genetic, Twins, Monozygotic, Cardiovascular disease, DNA methylation, Epigenetics, acyl coenzyme A synthetase family member 3, acyl coenzyme A synthetase medium chain family member 2A, carboxylesterase 1, carboxylesterase 1 pseudogene 1, creatine kinase, enzyme, genomic DNA, hydrolase, iron sulfur cluster assembly enzyme, lactate dehydrogenase, lipid droplet associated hydrolase, microsomal triglyceride transfer protein, SEC14 like lipid binding 2, unclassified drug

الوصف: Background/Aim: This study aimed to measure the DNA methylation state of thousands of CpG islands in the blood of two monozygotic twins that were discordant for cardiovascular disease (CVD). Twin 1 had suffered myocardial infarction, while the other was healthy. Patients and Methods: Since the aim of this study was to identify differentially methylated regions which might act as potential markers, reduced-representation bisulfite libraries were used for whole-genome methylation analysis. Results: According to the analysis, 11 genes lipid droplet associated hydrolase (LDAH), apolipoprotein B (APOB), acyl-CoA synthetase medium chain family member 2A (ACSM2A), acyl-CoA synthetase medium chain family member 5(ACSM5), acyl-CoA synthetase family member 3 (ACSF3), carboxylesterase 1 (CES1), carboxylesterase 1 pseudogene 1 (CES1P1), AFG3 like matrix AAA peptidase subunit 2 (AFG3L2), iron-sulfur cluster assembly enzyme (ISCU), SEC14 like lipid binding 2 (SEC14L2) and microsomal triglyceride transfer protein (MTTP) were all hypomethylated in DNA from twin 2, the unaffected twin. Methylation changes were observed at different multiple loci between the twins, suggesting loci that are affected by disease status in identical genetic backgrounds. Conclusion: This twin study may contribute significantly to the understanding of the genetic basis of CVD and resulting myocardial infarction. This approach may allow identification of possible target loci associated with aberrant epigenetic regulation in CVD. © 2020 International Institute of Anticancer Research. All rights reserved.

العلاقة: In Vivo; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://hdl.handle.net/11499/37524Test; https://doi.org/10.21873/invivo.11782Test; 34; 361; 367; 2-s2.0-85077264159; WOS:000504753200044

الإتاحة: https://doi.org/10.21873/invivo.11782Test
https://hdl.handle.net/11499/37524Test

المؤلفون: Marco Morselli, Aylin Köseler, Matteo Pellegrini, Feiyang Ma, Oguz Kilic, Ismail Dogu Kilic

المصدر: In vivo (Athens, Greece), vol 34, iss 1

مصطلحات موضوعية: Male, Cancer Research, genomic DNA, Twins, Myocardial Infarction, microsomal triglyceride transfer protein, multidetector computed tomography, Cardiovascular, Microsomal triglyceride transfer protein, anterior myocardial infarction, Epigenesis, Genetic, genetic background, 0302 clinical medicine, aspartate aminotransferase, SEC14 like lipid binding 2, T lymphocyte, apolipoprotein B, echocardiography, genetics, Promoter Regions, Genetic, genome analysis, Genetics, clinical article, Genome, DNA methylation, reduced representation bisulfite sequencing, adult, iron sulfur cluster assembly enzyme, neutrophil, Methylation, gene expression regulation, Cardiovascular disease, carboxylesterase 1, Prognosis, unclassified drug, Heart Disease, Phenotype, CpG site, fatty acid metabolism, twin discordance, 030220 oncology & carcinogenesis, monocyte, Epigenetics, acyl coenzyme A synthetase family member 3, genetic marker, Research Article, Genetic Markers, gene locus, Carboxylesterase 1, electrocardiography, Clinical Sciences, heart infarction, Biology, General Biochemistry, Genetics and Molecular Biology, Article, genetic epigenesis, Monozygotic, Promoter Regions, enzyme blood level, 03 medical and health sciences, carboxylesterase 1 pseudogene 1, monozygotic twins, promoter region, Genetic, blood, human genome, gene expression profiling, case report, Humans, Oncology & Carcinogenesis, human, acyl coenzyme A synthetase medium chain family member 2A, Heart Disease - Coronary Heart Disease, Pharmacology, B lymphocyte, creatine kinase, Genome, Human, human cell, percutaneous coronary intervention, lactate dehydrogenase, Twins, Monozygotic, Twin study, lipid droplet associated hydrolase, enzyme, Differentially methylated regions, acyl coenzyme A synthetase medium chain family member 5, biology.protein, cholesterol metabolism, CpG island, AFG3 like matrix AAA peptidase subunit 2, hydrolase, coronary artery obstruction, Epigenesis

الوصف: Background/Aim: This study aimed to measure the DNA methylation state of thousands of CpG islands in the blood of two monozygotic twins that were discordant for cardiovascular disease (CVD). Twin 1 had suffered myocardial infarction, while the other was healthy. Patients and Methods: Since the aim of this study was to identify differentially methylated regions which might act as potential markers, reduced-representation bisulfite libraries were used for whole-genome methylation analysis. Results: According to the analysis, 11 genes lipid droplet associated hydrolase (LDAH), apolipoprotein B (APOB), acyl-CoA synthetase medium chain family member 2A (ACSM2A), acyl-CoA synthetase medium chain family member 5(ACSM5), acyl-CoA synthetase family member 3 (ACSF3), carboxylesterase 1 (CES1), carboxylesterase 1 pseudogene 1 (CES1P1), AFG3 like matrix AAA peptidase subunit 2 (AFG3L2), iron-sulfur cluster assembly enzyme (ISCU), SEC14 like lipid binding 2 (SEC14L2) and microsomal triglyceride transfer protein (MTTP) were all hypomethylated in DNA from twin 2, the unaffected twin. Methylation changes were observed at different multiple loci between the twins, suggesting loci that are affected by disease status in identical genetic backgrounds. Conclusion: This twin study may contribute significantly to the understanding of the genetic basis of CVD and resulting myocardial infarction. This approach may allow identification of possible target loci associated with aberrant epigenetic regulation in CVD. © 2020 International Institute of Anticancer Research. All rights reserved.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82cb2c54ba67a102c40f00d3e1e8a8b7Test
https://hdl.handle.net/11499/37524Test

المؤلفون: Rui Xiao, Yi Sheng

المصدر: Cancer Research. 78:461-461

مصطلحات موضوعية: Stress (mechanics), Cancer Research, Oncology, Iron-sulfur cluster assembly enzyme, Chemistry, Biophysics

الوصف: Iron is essential for animal cellular homeostasis by acting in the biogenesis of two important redox-reactive prosthetic groups of enzymes: iron sulfur clusters (ISC) and heme. Previous studies have indicated that the misregulated iron metabolism is associated with multiple aging-related neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease. However, how iron metabolism modulates the aging process remains largely elusive.In the current study, we aim to reveal the roles and mechanisms of iron metabolism in the aging process of C. elegans, a well-established genetic model organism for aging research. Through our preliminary studies, we have found that the iron-sulfur cluster assembly gene isu-1 plays an important role in the lifespan modulation and stress resistance of C. elegans. Specifically, RNAi knocking-down of isu-1 causes a significantly upregulated mitochondrial unfolded protein response (mitoUPR). Furthermore, the isu-1 RNAi-treated worms are significantly more resistant to heat shock and oxidative stress. Lastly, isu-1 RNAi significantly extends lifespan (~25.7%).Currently, we are examining the transcription factors downstream of isu-1 in regulating aging and stress responses. Surprisingly, the isu-1 RNAi-triggered lifespan extension is independent of insulin and IGF pathway, an evolutionarily conserved master regulator of animal growth and aging. Next, we will characterize the mechanisms underlying the effects of iron-sulfur cluster in animal aging and stress response using various genetic and biochemistry approaches. Citation Format: Rui Xiao, Yi Sheng. An essential role of the iron-sulfur cluster assembly enzyme isu-1 in the aging and stress responses of C. elegans [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2018; 2018 Apr 14-18; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2018;78(13 Suppl):Abstract nr 461.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::744b031675b8cba7cbdbf6df28292dcbTest
https://doi.org/10.1158/1538-7445.am2018-461Test