المؤلفون: Попель, О.К., Говсєєв, Д.О.

المصدر: Reproductive health of woman; No. 1 (2024); 32-41 ; Reproductive health of woman; № 1 (2024); 32-41 ; Репродуктивне здоров'я жінки; № 1 (2024); 32-41 ; 2708-8731 ; 2708-8723

مصطلحات موضوعية: HSP70, heat shock protein 70, прееклампсія, накладена прееклампсія, хронічна гіпертензія, вагітність, heat-shock protein 70, preeclampsia, superimposed preeclampsia, chronic hypertension, pregnancy

الوصف: The objective: to investigate serum heat shock protein 70 (HSP70) concentration in pregnant women with chronic hypertension and superimposed preeclampsia. To assess the prospects of using HSP70 as a superimposed preeclampsia predictor.Materials and methods. The original prospective cohort single-center observational study included 105 pregnant women with chronic hypertension and 34 healthy pregnant women as a control group. Serum HSP70 was measured via enzyme-linked immunosorbent assay. The first measurement point of serum HSP70 was at 28 weeks of gestation, the second measurement point was at 36 weeks in case of absence of preeclampsia or at 29-35 weeks in case of preeclampsia. If signs of preeclampsia appeared later than 36 weeks, additional HSP70 measurement was not performed. Statistical analysis was conducted using EZR 1.55 software.Results. In the study group (105 pregnant women with chronic hypertension), after delivery 30 patients had signs of superimposed preeclampsia (superimposed preeclampsia subgroup) and 75 persons had no signs of superimposed preeclampsia (chronic hypertension subgroup). In the control group (34 healthy pregnant women) 3 patients after delivery had preeclampsia signs (preeclampsia subgroup) and another 31 had no signs of preeclampsia (healthy subgroup).Serum HSP70 levels, obtained at the first measurement point (28 weeks) was statistically significantly different between three subgroups: patients with chronic hypertension vs. patients with superimposed preeclampsia (p<0.01), healthy pregnant women vs. chronic hypertension women (p<0.0001), healthy persons vs. superimposed preeclampsia persons (p<0.0001).Serum HSP70 concentrations at the second measurement point (36 weeks, or 29-35 weeks in case of preeclampsia) also had a statistically significant difference for each pair of subgroups (p<0.001). Given the small number of persons in the preeclampsia subgroup (3 women), it was not included in the calculations.No statistically significant difference between serum HSP70 ...

وصف الملف: application/pdf

العلاقة: https://repro-health.com.ua/article/view/301594/293991Test; https://repro-health.com.ua/article/view/301594Test

الإتاحة: https://repro-health.com.ua/article/view/301594Test

المؤلفون: Riess, Jonathan W, Reckamp, Karen L, Frankel, Paul, Longmate, Jeffrey, Kelly, Karen A, Gandara, David R, Weipert, Caroline M, Raymond, Victoria M, Keer, Harold N, Mack, Philip C, Newman, Edward M, Lara, Primo N

المصدر: Clinical Lung Cancer. 22(6)

مصطلحات موضوعية: Clinical Research, Clinical Trials and Supportive Activities, Lung, Cancer, Lung Cancer, 6.2 Cellular and gene therapies, 6.1 Pharmaceuticals, Evaluation of treatments and therapeutic interventions, Good Health and Well Being, Aged, Benzamides, California, ErbB Receptors, Erlotinib Hydrochloride, Female, Humans, Isoindoles, Lactates, Lung Neoplasms, Male, Middle Aged, Mutation, Prospective Studies, Heat shock protein inhibitors, circulating tumor DNA, EGFR Exon 20 insertion, Heat shock protein 90, EGFR tyrosine kinase inhibitor, Clinical Sciences, Oncology and Carcinogenesis, Oncology & Carcinogenesis

الوصف: BackgroundOnalespib is a novel heat shock protein 90 inhibitor (HSP90i). Previous preclinical and clinical studies with HSP90i have demonstrated activity in EGFR-mutant non-small cell lung cancer (NSCLC). This study sought to determine the safety and tolerability of onalespib plus erlotinib in EGFR-mutant NSCLC and to evaluate the preliminary efficacy of the combination in epidermal growth factor receptor exon 20 insertion (EGFRex20ins) NSCLC.Patients and methodsStandard 3+3 dose escalation was followed by a phase II expansion in EGFRex20ins. The phase II component targeted a response rate of 25% versus a background rate of 5%. Prospective next-generation sequencing (NGS) of 70 cancer-related genes, including EGFR, via plasma circulating tumor DNA (ctDNA) was performed. Toxicity was graded by Common Terminology Criteria for Adverse Events (CTCAE), version 4, and response was determined by Response Evaluation Criteria in Solid Tumours (RECIST) 1.1.ResultsEleven patients were treated (nine dose escalation, two dose expansion). Two dose-limiting toxicities (DLTs) occurred in dose level (DL) 0 and zero in DL -1 (minus). In 10 EGFRex20ins patients, no responses were observed, median progression-free survival was 5.4 months (95% confidence interval, 0.9-5.7), and the disease control rate (DCR) was 40% (median, 3.5 months). EGFRex20ins was detected in nine of 10 ctDNA samples at baseline; on-treatment ctDNA clearance was not observed. Grade 3 diarrhea was the predominant toxicity in 45% of patients. The recommended phase II dose is DL -1 (minus): erlotinib 150 mg orally every morning and onalespib 120 mg/m2 intravenously on days 1, 8, and 15 every 28 days.ConclusionOverlapping toxicities of erlotinib and onalespib, mainly diarrhea, limited the tolerability of this combination, and limited clinical activity was observed, so the trial was closed early. Plasma EGFRex20ins ctDNA was detected in the majority of patients; failure to clear ctDNA was consistent with lack of tumor response (NCT02535338).

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/2fh7k2thTest

المؤلفون: Rakshit K Dalal, Manpreet Kaur, Komal Khatri, Foram Patel, Heena Shaikh, Arifa Bakerywala

المصدر: Journal of Pharmacy and Bioallied Sciences, Vol 16, Iss 5, Pp 498-500 (2024)

مصطلحات موضوعية: cardiovascular diseases, elisa, heat shock protein, periodontitis, serum level, Pharmacy and materia medica, RS1-441, Analytical chemistry, QD71-142

الوصف: Introduction: HSP is arguably the most thoroughly studied self-antigens connected to Cardio Vascular Diseases (CVD) and periodontal disease. Hence, the major goal of this analysis was to determine the amount of HSP60 in patients' Chronic Periodontitis (CP) patients' serum. Materials and Methods: The current investigation involved 100 patients in all. Based on the patients' periodontal and cardiovascular health, the patients were divided. The patients were made aware that this research had no direct bearing on disease treatment or cure. Results: In contrast to periodontal disease, which had a mean serum HSP60 of 59.94 ng/dl, CVD had a mean serum HSP60 of 85.98 ng/dl. When compared to periodontal disease, the CVD increased significantly (P < 0.05, 0.03). Discussion and Conclusion: We emphasize the function of HSP60 in the pathophysiology of individuals with chronic periodontitis based on the findings of the current investigation. Serum HSP60 concentrations can serve as a biomarker for periodontal inflammation. More longitudinal and interventional research with a larger sample size is required to validate the present findings. In periodontal therapies, targeting HSP60 may enhance results.

وصف الملف: electronic resource

العلاقة: http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2024;volume=16;issue=5;spage=498;epage=500;aulast=DalalTest; https://doaj.org/toc/0975-7406Test

الوصول الحر: https://doaj.org/article/89096dc051cf4ffdb2bdb42a0eb99571Test

المؤلفون: Heba A. Elhendawy

المصدر: European Journal of Medical Research, Vol 28, Iss 1, Pp 1-18 (2023)

مصطلحات موضوعية: Oral squamous cell carcinoma, Heat shock protein 70, Immunohistochemistry, Prognostic indicators, Recurrence, DFS, Medicine

الوصف: Abstract Background Oral cancer is a common cause of death worldwide. The search for novel biomarkers for oral cancer is an ongoing struggle. Prognostic biomarkers are of great importance in diagnosis, and prediction of the cancer outcome. There are several disagreements in oral cancer studies over the role of heat shock proteins as prognostic markers. The current study investigated HSP70 expression in diverse tissues ranging from normal oral mucosa to dysplastic oral epithelium and oral squamous cell carcinoma to determine its role in oral carcinogenesis. Moreover, HSP70 was evaluated concerning different prognostic parameters to determine its capability in predicting cancer progression. Recurrence of tumor was recorded, and patients` disease-free survival was calculated and analyzed considering HSP70 expression to determine the potential utility of HSP70 immuno-expression in predicting recurrence. Methods A retrospective study was accomplished on 50 cases of OSCC. Biopsies from the cancerous tissue, the free surgical margin, and the normal oral mucosa were used. The grading of dysplastic epithelium and OSCCs followed the criteria of WHO classification (2017). The clinicopathological and follow-up records for each patient were retrieved. Pearson’s Chi-square test, one-way ANOVA, and post hoc tests were used to analyze the variance of HSP70 immuno-expression concerning different parameters. The Kaplan–Meier method was used to compute and visualize disease-free survival, and the log-rank test was used to analyze the data. With Cox regression, univariate and multivariate survival analyses were run. A P-value of 0.05 or less was regarded as statistically significant. Results A significant increased expression of HSP70 was observed as the tissue progressed from normal to dysplastic epithelium, and carcinoma (P = 0.000). HSP70 revealed a significant increased expression by progression from mild to severe dysplasia (P = 0.023), and also from well to moderately and poorly differentiated carcinoma (P = 0.000). High HSP70 immuno-expression was significantly associated with progression of OSCC; large-sized tumors (P = 0.002), advanced TNM clinical stages (P = 0.001), positive nodal involvement (P = 0.001), presence of recurrence (P = .008), and reduced DFS (P = 0.014). Conclusion HSP70 has a crucial contribution to oral carcinogenesis, and its immune-expression could potentially be used as predictor of progression and recurrence of OSCC patients. Trial registration: Retrospectively registered.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2047-783XTest

الوصول الحر: https://doaj.org/article/bb4e955d9c9145bcb2d114bf95e7c722Test

المؤلفون: Zihao Wang, Fang Cheng, Yuxiu Xu, Xin Li, Songdong Meng

المصدر: Biosafety and Health, Vol 5, Iss 5, Pp 280-288 (2023)

مصطلحات موضوعية: Innate immunity, SARS-CoV-2, Traditional Chinese medicine, Depression, Heat shock protein, Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270

الوصف: During severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, activated macrophages, dendritic cells (D.C.), neutrophils, and natural killer (N.K.) cells are the first defense against infection. These immune effectors trap and ingest the virus, kill infected epithelial cells, or produce anti-viral cytokines. Evidence suggests that aging, obesity, and mental illness can lead to weakened innate immunity and, thus, are all associated with elevated infection and severe disease progression of coronavirus disease 2019 (COVID-19). Innate immune defense networks play a fundamental role in suppressing viral replication, infection establishment, and viral pathogenesis of SARS-CoV-2 and other respiratory viruses.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2590053623001076Test; https://doaj.org/toc/2590-0536Test

الوصول الحر: https://doaj.org/article/960048f50ba74ffc8c123c61ce1f3e83Test

المؤلفون: Hyo Bin Lee, Hyun Seok Jang, Young Dae Oh, Yun Ho Lee, Han Kyu Lim

المصدر: Journal of the World Aquaculture Society, Vol 54, Iss 5, Pp 1407-1426 (2023)

مصطلحات موضوعية: glucose, Haliotis discus hannai, heat shock protein, Pacific abalone, stress response, Aquaculture. Fisheries. Angling, SH1-691

الوصف: Abstract The Pacific abalone (Haliotis discus hannai) is the most economically valuable shellfish species in Northeast Asia, accounting for most of the shellfish production in South Korea. Despite the continuous improvement in the productivity of domestic abalone aquaculture, rapid seawater temperature changes caused by global warming have led to mass mortality in coastal abalone farms. Therefore, research on environmental stressors is urgently needed to prevent abalone production losses. This study analyzed the expression of heat shock protein (HSP) genes in foot muscles of H. discus hannai and metabolic parameters including glucose, lysozyme, catalase, D‐lactate, and trehalose levels in the hemolymph under acute high temperature, low temperature, low salinity, and air exposure. Quantitative reverse‐transcription polymerase chain reaction analysis showed that HSP20 expression was downregulated in all experimental groups, whereas HSP70 expression increased immediately in all experimental groups except for the low‐temperature stress group. Hemolymph analysis showed that glucose and lysozyme levels increased significantly immediately after treatment in all groups, and decreased during the recovery period. Together, the results indicate that low water temperature stress had the smallest effect on abalone among the treatments. We conclude that the other treatments acted as stressors of abalone and that HSP genes, glucose, and lysozyme can be used as stress indicators in H. discus hannai.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0893-8849Test; https://doaj.org/toc/1749-7345Test

الوصول الحر: https://doaj.org/article/484c41478abc4c25914a420619557213Test

المؤلفون: J. Corbett, J. S. Young, M. J. Tipton, J. T. Costello, T. B. Williams, E. F. Walker, B. J. Lee, C. E. Stevens

المصدر: Journal of Physiological Sciences, Vol 73, Iss 1, Pp 1-24 (2023)

مصطلحات موضوعية: Heat, Acclimation, Acclimatisation, Hormone, Heat shock protein, Physiology, QP1-981

الوصف: Abstract Heat acclimation/acclimatisation (HA) mitigates heat-related decrements in physical capacity and heat-illness risk and is a widely advocated countermeasure for individuals operating in hot environments. The efficacy of HA is typically quantified by assessing the thermo-physiological responses to a standard heat acclimation state test (i.e. physiological biomarkers), but this can be logistically challenging, time consuming, and expensive. A valid molecular biomarker of HA would enable evaluation of the heat-adapted state through the sampling and assessment of a biological medium. This narrative review examines candidate molecular biomarkers of HA, highlighting the poor sensitivity and specificity of these candidates and identifying the current lack of a single ‘standout’ biomarker. It concludes by considering the potential of multivariable approaches that provide information about a range of physiological systems, identifying a number of challenges that must be overcome to develop a valid molecular biomarker of the heat-adapted state, and highlighting future research opportunities.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1880-6562Test

الوصول الحر: https://doaj.org/article/ae65fc652a7944a48f563f45ce237b1bTest

المؤلفون: Yin X, Zhang H, Wei Z, Wang Y, Han S, Zhou M, Xu W, Han W

المصدر: Cancer Management and Research, Vol Volume 15, Pp 1165-1179 (2023)

مصطلحات موضوعية: crotonylation, hypoxia, oral squamous cell carcinoma, glycolysis, heat shock protein 90 alpha family class b member 1, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Xiteng Yin,1,2 Hongbo Zhang,1,2 Zheng Wei,2,3 Yufeng Wang,1,2 Shengwei Han,1,2 Meng Zhou,4 Wenguang Xu,1,2 Wei Han1,2 1Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, People’s Republic of China; 2Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, People’s Republic of China; 3Pediatric Dentistry, Nanjing Stomatology Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, People’s Republic of China; 4Department of Oral and Maxillofacial Surgery, the Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou, People’s Republic of ChinaCorrespondence: Wei Han; Wenguang Xu, Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, People’s Republic of China, Tel/Fax +86 25 8362 0140 ; +86-025-83620202, Email doctorhanwei@hotmail.coml; wenguang.xu@foxmail.comPurpose: Lysine crotonylation, an emerging posttranslational modification, has been implicated in the regulation of diverse biological processes. However, its involvement in oral squamous cell carcinoma (OSCC) remains elusive. This study aims to reveal the global crotonylome in OSCC under hypoxic conditions and explore the potential regulatory mechanism of crotonylation in OSCC.Methods: Liquid-chromatography fractionation, affinity enrichment of crotonylated peptides, and high-resolution mass spectrometry were employed to detect differential crotonylation in CAL27 cells cultured under hypoxia. The obtained data were further subjected to bioinformatics analysis to uncover the involved biological processes and pathways of the dysregulated crotonylated proteins. A site-mutated plasmid was utilized to investigate the effect of crotonylation on Heat Shock Protein 90 Alpha Family Class B Member 1 (HAP90AB1) function.Results: A large-scale crotonylome analysis revealed 1563 crotonylated modification sites on 605 proteins in CAL27 cells under hypoxia. Bioinformatics analysis revealed a significant decrease in histone crotonylation levels, while up-regulated crotonylated proteins were mainly concentrated in non-histone proteins. Notably, glycolysis-related proteins exhibited prominent up-regulation among the identified crotonylated proteins, with HSP90AB1 displaying the most significant changes. Subsequent experimental findings confirmed that mutating lysine 265 of HSP90AB1 into a silent arginine impaired its function in promoting glycolysis.Conclusion: Our study provides insights into the crotonylation modification of proteins in OSCC under hypoxic conditions and elucidates the associated biological processes and pathways. Crotonylation of HSP90AB1 in hypoxic conditions may enhance the glycolysis regulation ability in OSCC, offering novel perspectives on the regulatory mechanism of crotonylation in hypoxic OSCC and potential therapeutic targets for OSCC treatment.Keywords: crotonylation, hypoxia, oral squamous cell carcinoma, glycolysis, heat shock protein 90 alpha family class B member 1

وصف الملف: electronic resource

العلاقة: https://www.dovepress.com/large-scale-identification-of-lysine-crotonylation-reveals-its-potenti-peer-reviewed-fulltext-article-CMARTest; https://doaj.org/toc/1179-1322Test

الوصول الحر: https://doaj.org/article/9ed2039e37d04ef585066313bb95d846Test

المؤلفون: Emmanuel Ogbodo, Francesco Michelangeli, John H. H. Williams

المصدر: FEBS Open Bio, Vol 13, Iss 10, Pp 1922-1940 (2023)

مصطلحات موضوعية: cytokine, differentiated U937 cells, heat shock protein, immune response, Naïve U937 cells, PBMCs, Biology (General), QH301-705.5

الوصف: Endogenous molecules, such as heat shock proteins (HSP), can function as danger signals when released into the extracellular environment in response to cell stress, where they elicit an immune response such as cytokine secretion. There has also been some suggestion that contamination of exogenous HSPs with lipopolysaccharide (LPS) may be responsible for these effects. This study investigates the effects of exogenous HSPA1A and HSPB1 on the activation of immune cells and the resulting secretion of cytokines, which are involved in inflammatory responses. To address whether exogenous HSPs can directly activate cytokine secretion, naïve U937 cells, differentiated U937 cells and peripheral blood mononuclear cells (PBMCs) were treated with either exogenously applied HSPA1A or HSPB1 and then secreted IL‐1β, TNF‐α and IL‐10 were measured by ELISA. Both HSPs were able to induce a dose‐dependent increase in IL‐10 secretion from naïve U937 cells and dose‐dependent IL‐1β, TNF‐α and IL‐10 secretion were also observed in differentiated U937 cells and PBMCs. We also observed that CD14 affects the secretion levels of IL‐1β, TNF‐α and IL‐10 from cells in response to exogenous HSP treatment. In addition, HSPA1A and HSPB1 were shown to interact with CD14, CD36 and CD11b extracellular receptor proteins. Several approaches used in this study indicate that HSP‐induced cytokine secretion is largely independent of any contaminating LPS in the samples.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2211-5463Test

الوصول الحر: https://doaj.org/article/969b7896bf3f4d9aac42d911fecbde5aTest

المؤلفون: Wei-Wei Wang, Hai-Yan Li, Huan-Huan Yan

المصدر: International Journal of Ophthalmology, Vol 16, Iss 10, Pp 1589-1594 (2023)

مصطلحات موضوعية: heat shock protein 47, filtration surgery, conjunctival bleb, scar, transforming growth factor-β1, Ophthalmology, RE1-994

الوصف: AIM: To evaluate the effectiveness of knock-down of heat shock protein 47 (HSP47) on conjunctival bleb scarring in a rat model and its possible mechanism. METHODS: Male Sprague–Dawley rats were used for glaucoma filtration surgery (GFS) and were treated with either phosphate buffered solution, shControl, mitomycin C, or sh-HSP47 using a microsyringe immediately after GFS. The morphology of filtering blebs was observed postoperatively. The levels of HSP47 were analyzed at 2, 5, 8, and 11d after GFS via real time quantitative polymerase chain reaction (PCR) and Western blot. The silencing effect of HSP47, the expression of collagen I and III, and the potential signaling pathways of HSP47 during scarification were explored 11d post GFS. The protein levels of transforming growth factor-β1 (TGF-β1), phospho-Smad2 (pSmad2), phospho-Smad3 (p-Smad3), and phospho-p38 (p-p38) were also analyzed using Western blot. RESULTS: Sh-HSP47 treatment significantly prolonged the functional filtration bleb retention. The levels of HSP47 were increased significantly at 5, 8, and 11d postoperatively compared to the control group (P

وصف الملف: electronic resource

العلاقة: http://ies.ijo.cn/en_publish/2023/10/20231005.pdfTest; https://doaj.org/toc/2222-3959Test; https://doaj.org/toc/2227-4898Test

الوصول الحر: https://doaj.org/article/e635047951224a3c9326d77ea7efd03aTest