المؤلفون: Xiaoyang Bi, Meihong Lin, Yifeng Zhou, Dandan Li, Zuowei Xu, Lijuan Zhou, Jiguang Huang

مصطلحات موضوعية: Biochemistry, Microbiology, Cell Biology, Genetics, Biotechnology, Ecology, Environmental Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, via hydrogen bonds, traditional chemical pesticides, fourth instar larvae, environmentally friendly approach, aryltetralin lignan lactone, 4 ′- dimethylamentoflavone, aedes aegypti <, gaussen botanical insecticides, taiwania flousiana <, 50 , c3 ), flousiana <, novel insecticides, gaussen (<, c5 ), c4 ), studies found, shallow matrices, selective channel, results indicated, potential target

الوقت: 7

الوصف: Botanical insecticides are considered an environmentally friendly approach to insect control because they are easily biodegraded and cause less environmental pollution compared to traditional chemical pesticides. In this study, we reported the insecticidal activities of the ingredients from Taiwania flousiana Gaussen ( T. flousiana ). Five compounds, namely helioxanthin (C1), taiwanin E (C2), taiwanin H (C3), 7,4′-dimethylamentoflavone (C4), and 7,7″-di- O -methylamentoflavone (C5), were isolated and tested against the second, third, and fourth instar larvae of Aedes aegypti . Our results indicated that all five compounds showed insecticidal activities, and helioxanthin, which is an aryltetralin lignan lactone, was the most effective with LC 50 values of 0.60, 2.82, and 3.12 mg/L, respectively, 48 h after application, with its activity against the second instar larvae similar to that of pyrethrin and better than that of rotenone. Further studies found that helioxanthin accumulated in the gastric cecum and the midgut and caused swelling of mitochondria with shallow matrices and fewer or disappeared crista. Additionally, our molecular mechanisms studies indicated that the significantly differentially expressed genes (DEGs) were mainly associated with mitochondria and the cuticle, among which the voltage-dependent anion-selective channel (VDAC) gene was the most down-regulated by helioxanthin, and VDAC is the potential target of helioxanthin by binding to specific amino acid residues (His 122 and Glu 147) via hydrogen bonds. We conclude that aryltetralin lignan lactone is a potential class of novel insecticides by targeting VDAC.

العلاقة: https://figshare.com/articles/journal_contribution/Insecticidal_Activity_and_Molecular_Target_by_Morphological_Analysis_RNAseq_and_Molecular_Docking_of_the_Aryltetralin_Lignan_Lactone_Helioxanthin_Isolated_from_i_Taiwania_flousiana_i_Gaussen/25326258Test

الإتاحة: https://doi.org/10.1021/acs.jafc.3c06384.s001Test
https://figshare.com/articles/journal_contribution/Insecticidal_Activity_and_Molecular_Target_by_Morphological_Analysis_RNAseq_and_Molecular_Docking_of_the_Aryltetralin_Lignan_Lactone_Helioxanthin_Isolated_from_i_Taiwania_flousiana_i_Gaussen/25326258Test

المؤلفون: Hongmei Liu, Jiguang Huang, Sifan Yang, Jialin Li, Lijuan Zhou

المصدر: Molecules; Volume 25; Issue 4; Pages: 967

مصطلحات موضوعية: Taiwania flousiana Gaussen, essential oil, algicidal activity, antimicrobial activity, antioxidant activity, algicidal mechanism

جغرافية الموضوع: agris

الوصف: Taiwania flousiana (T. flousiana) Gaussen is a precious wood in the family Taxodiaceae. This study investigated the chemical components of the essential oil from the stem bark of T. flousiana and its algicidal, antifungal, and antioxidant properties. Sixty-nine compounds representing 89.70% of the stem bark essential oil were identified by GC-MS. The essential oil showed strong anti-algae, anti-bacteria, and anti-fungus activities against the tested species, and antioxidant activities. The IC50 values of the essential oil against chlorophyll a, chlorophyll b, and the total chlorophyll of Spirogyra communis (a species of algae), 24–96 h after the treatment, ranged from 31.77 to 84.92 μg/mL, while the IC50 values of butachlor ranged from 40.24 to 58.09 μg/mL. Ultrastructure changes revealed by the transmission electron microscopy indicated that the main algicidal action sites were the chloroplast and cell wall. The essential oil showed antifungal activities on Rhizoctonia solani (EC50 = 287.94 μg/mL) and Colletotrichum gloeosporioiles (EC50 = 378.90 μg/mL). It also showed bactericidal activities on Ralstonia solanacearum and Staphylococcus aureus, with zones of inhibition (ZOIs) being 18.66 and 16.75 mm, respectively at 40 μg/disk. Additionally, the essential oil possessed antioxidant activity estimated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method (IC50 = 33.51 μg/mL; IC50 value of the positive control ascorbic acid was 7.98 μg/mL). Thus, the essential oil of this plant might be used as a possible source of natural bioactive molecules in agrochemical industry as well as in food and cosmetic industries.

وصف الملف: application/pdf

العلاقة: https://dx.doi.org/10.3390/molecules25040967Test

الإتاحة: https://doi.org/10.3390/molecules25040967Test

المؤلفون: Qinghua Wang, Yunqing Li, Xiaolong Yuan, Yi Wang

المصدر: Mitochondrial DNA. Part B. Resources, Vol 5, Iss 1, Pp 1040-1041 (2020)

مصطلحات موضوعية: taiwania flousiana, chloroplast, illumina sequencing, phylogenetic analysis, Genetics, QH426-470

الوصف: The first complete chloroplast genome (cpDNA) sequence of Taiwania flousiana was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 132,565 bp in length, contains a large single copy region (LSC) of 72,930 bp and a small single copy region (SSC) of 59,477 bp, which were separated by a pair of inverted repeats (IR) regions of 79 bp. The genome contains 121 genes, including 83 protein-coding genes, 4 ribosomal RNA genes, and 34 transfer RNA genes. Further phylogenomic analysis showed that T. flousiana and Taiwania cryptomerioides clustered in a clade in Cupressaceae family.

العلاقة: http://dx.doi.org/10.1080/23802359.2020.1721355Test; https://doaj.org/toc/2380-2359Test; https://doaj.org/article/0c2c8033cb354a9a92868f75ebc01c08Test

الإتاحة: https://doi.org/10.1080/23802359.2020.1721355Test
https://doaj.org/article/0c2c8033cb354a9a92868f75ebc01c08Test

المؤلفون: Hongmei, Liu, Jiguang, Huang, Sifan, Yang, Jialin, Li, Lijuan, Zhou

المصدر: Molecules

مصطلحات موضوعية: Staphylococcus aureus, algicidal activity, antimicrobial activity, Cupressaceae, antioxidant activity, algicidal mechanism, Spirogyra, Antioxidants, Article, essential oil, Rhizoctonia, Anti-Infective Agents, Colletotrichum, Oils, Volatile, Ralstonia solanacearum, Taiwania flousiana Gaussen

الوصف: Taiwania flousiana (T. flousiana) Gaussen is a precious wood in the family Taxodiaceae. This study investigated the chemical components of the essential oil from the stem bark of T. flousiana and its algicidal, antifungal, and antioxidant properties. Sixty-nine compounds representing 89.70% of the stem bark essential oil were identified by GC-MS. The essential oil showed strong anti-algae, anti-bacteria, and anti-fungus activities against the tested species, and antioxidant activities. The IC50 values of the essential oil against chlorophyll a, chlorophyll b, and the total chlorophyll of Spirogyra communis (a species of algae), 24–96 h after the treatment, ranged from 31.77 to 84.92 μg/mL, while the IC50 values of butachlor ranged from 40.24 to 58.09 μg/mL. Ultrastructure changes revealed by the transmission electron microscopy indicated that the main algicidal action sites were the chloroplast and cell wall. The essential oil showed antifungal activities on Rhizoctonia solani (EC50 = 287.94 μg/mL) and Colletotrichum gloeosporioiles (EC50 = 378.90 μg/mL). It also showed bactericidal activities on Ralstonia solanacearum and Staphylococcus aureus, with zones of inhibition (ZOIs) being 18.66 and 16.75 mm, respectively at 40 μg/disk. Additionally, the essential oil possessed antioxidant activity estimated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method (IC50 = 33.51 μg/mL; IC50 value of the positive control ascorbic acid was 7.98 μg/mL). Thus, the essential oil of this plant might be used as a possible source of natural bioactive molecules in agrochemical industry as well as in food and cosmetic industries.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::b0daac645a4495226b0ef11f3e31d32cTest
https://pubmed.ncbi.nlm.nih.gov/32093424Test

المؤلفون: Byk, Adam, Matusiak, Andrzej, Taszakowski, Artur, Szczepański, Wojciech T., Walczak, Marcin, Bunalski, Marek, Karpiński, Lech

مصطلحات موضوعية: Plantae, Tracheophyta, Pinopsida, Pinales, Cupressaceae, Juniperus, Pachytheca, Pinophyta, Pseudolarix erensis, Taiwania cryptomerioides flousiana, Cupressoideae, Magnoliopsida, Apiales, Apiaceae, Prangos, Ferula, Animalia, Arthropoda, Insecta, Coleoptera, Scarabaeoidea, Scarabaeidae, Onthophagus, Euonthophagus koshantschikoffi, Geotrupidae, Glaphyridae, Hybosoridae, new records, Rhyssemodes transcaspicus

الوصف: Figure 2 Typical landscapes in Tajikistan, habitats of scarabeoid beetles A mountain slope covered with shrubs including Juniperus in Iskanderkul environs B mountain meadow in Tojikobod environs C mountain slopes in Gharm environs D mountain meadow overgrown by Prangos and Ferula in Safed Dara environs E swarm of Onthophagus beetles in cow dung, Safed Dara environs F mountain slope covered with shrubs in Karatag environs G steep slope covered with single trees in Romit environs H watercourse near a pasture in Komsomolabad environs.

العلاقة: https://zenodo.org/communities/biosyslitTest; https://zenodo.org/record/4383511Test; https://doi.org/10.3897/zookeys.1003.55457.figure2Test; oai:zenodo.org:4383511

الإتاحة: https://doi.org/10.3897/zookeys.1003.55457.figure2Test
https://doi.org/10.3897/zookeys.1003.55457Test
https://zenodo.org/record/4383511Test

المؤلفون: Korobushkin, Daniil, Semenyuk, Irina, Tuf, Ivan

مصطلحات موضوعية: Plantae, Tracheophyta, Pinopsida, Pinales, Cupressaceae, JuniperusPachytheca, Pinophyta, Pseudolarix erensis, Taiwania cryptomerioides flousiana, Cupressoideae, Juniperus, Myriapoda, taxonomy, Utrish Nature Reserve, soil fauna

الوصف: Figure 1a - Photos of selected habitats in the Abrau Peninsula. A view of the coast of Utrish Nature Reserve

العلاقة: https://zenodo.org/communities/biosyslitTest; https://zenodo.org/record/911794Test; https://doi.org/10.3897/BDJ.4.e7308.figure1aTest; oai:zenodo.org:911794

الإتاحة: https://doi.org/10.3897/BDJ.4.e7308.figure1aTest
https://doi.org/10.3897/BDJ.4.e7308Test
https://zenodo.org/record/911794Test

المؤلفون: 朱麗萍, 潘富俊, 楊政川, 王亞男

المساهمون: 國立中興大學農學院實驗林管理處

مصطلحات موضوعية: RAPD, 臺灣杉, Taiwania cryptomerioides, Taiwania flousiana, Genetic variation, 禿杉, 遺傳變異

الوصف: 本研究以逢機擴大多型性(RAPD)來進行臺灣杉屬植物之遺傳變異分析：禿杉材料採自中國大陸福建屏南、貴州雷公山以及雲南騰衝；臺灣杉的材料則來自六龜研究中心20林班臺灣杉單親後裔試驗林木之雪山、望鄉及關山三個族群之種源，並以柳杉作為群外對照。RAPD電泳以POPGENE套裝軟體進行族群遺傳變異之相關分析，結果顯示臺灣杉之關山和望鄉地區族群的相似性指數最高，為0.9645，雪山和望鄉地區之相似性指數次之；而中國大陸地區之禿杉族群則以貴州和雲南之族群相關係數0.9228最高，福建和貴州地區族群之0.9050次之。 ; Genetic variation of the genus Taiwania was studied using RAPD markers. Sampling of flousiana Gaussen was made in its natural distribution areas, i.e. Fu-Jien, Guei-Jou and Yun-z Guei-Jou and Yun-nan was 0.9228, between Fu-Jien and Guei-Jou 0.9050. The ge

العلاقة: #PLACEHOLDER_PARENT_METADATA_VALUE#; 林業研究季刊, Volume 28, Issue 1, Page(s) 15-27.; http://hdl.handle.net/11455/74123Test

الإتاحة: http://hdl.handle.net/11455/74123Test

المؤلفون: 朱麗萍, 潘富俊, 楊政川, 王亞男

المساهمون: 景觀學系

مصطلحات موضوعية: 臺灣杉, 禿杉, 遺傳變異, RAPD, Taiwan cryptomerioides, Taiwan flousiana, Genetic variation

الوصف: 本研究以逢機擴大多型性(RAPD)來進行臺灣杉屬植物之遺傳變異分析：禿杉材料採自中國大陸福建屏南、貴州雷公山以及雲南騰衝；臺灣杉的材料則來自六龜研究中心20林班臺灣杉單親後裔試驗林木之雪山、望鄉及關山三個族群之種源，並以柳杉作為群外對照。RAPD電泳以POPGENE套裝軟體進行族群遺傳變異之相關分析，結果顯示臺灣杉之關山和望鄉地區族群的相似性指數最高，為0.9645，雪山和望鄉地區之相似性指數次之；而中國大陸地區之禿杉族群則以貴州和雲南之族群相關係數0.9228最高，福建和貴州地區族群之0.9050次之。依據樹狀圖分析，禿杉與臺灣杉之類群雖可以清楚的區分兩個類群，但兩類群之聯結點僅在11.1，顯見兩類群有非常大之相似性。中國大陸禿杉的遺傳歧異度為0.1744低於臺灣杉之0.2271，兩者總歧異度為0.2709，數值偏低。本研究用UPGMA樹枝狀圖得出兩類群間之遺傳相似度為0.8890，仍在種內族群間內之變異範圍，應可列於同一種群之下，但兩者之間的基因流Nm值低於1，為0.7553，顯示禿杉和臺灣杉之間有基因交流的障礙存在，而兩者亦顯示禿杉和臺灣杉之仍具有可資區別之定量及定性形態特徵。 Genetic variation of the genus Taiwania was studied using RAPD markers. Sampling of flousiana Gaussen was made in its natural distribution areas, i.e. Fu-Jien, Guei-Jou and Yun-z Guei-Jou and Yun-nan was 0.9228, between Fu-Jien and Guei-Jou 0.9050. The genetic diversity of T. flousiana was 0.1724, lower than that of T. cryptomerioides which was 0.2771. When pooling together, the genetic diversity was 0.2709. Based on the UPGMA phonogram, T. cryptomerioides and Taiwania flousiana could be divided into 2 different clusters. The SI between two species was as high as 0.8890, within the range of species level, suggesting they can be treated as the same species. However, Nm in the genus was as low as 0.7553. Besides, two taxa they still have consistent morphological difference, which can be used to distinguish each from other.

وصف الملف: 475 bytes; text/html

العلاقة: 林業研究季刊 28:1 民95.03 頁15-27; http://ir.lib.pccu.edu.tw//handle/987654321/39595Test; http://ir.lib.pccu.edu.tw/bitstream/987654321/39595/2/index.htmlTest

الإتاحة: http://ir.lib.pccu.edu.tw//handle/987654321/39595Test
http://ir.lib.pccu.edu.tw/bitstream/987654321/39595/2/index.htmlTest

المؤلفون: Jui-Lin, Chang

مرشدي الرسالة: Chi- Chu Tsai, Zin- Huang Liu, Chang- Hung Chou

مصطلحات موضوعية: genetic diversity, Taiwania flousiana Gaussen, internal transcribed spacers, inter- simple sequence repeat, Taiwania cryptomerioides Hayata

الوصف: The aim of this study was to obtain the molecular marker of Taiwania cryptomerioides Hayata based on DNA sequence data of PCR- sequencing and inter-simple sequence repeat (ISSR), and to evaluate the genetic diversity of populations of Taiwania cryptomerioides Hayata and molecular phylogeny of T. cryptomerioides and Taiwania flousiana Gaussen. The sequence data based on the internal transcribed spacer (ITS) of a total of 108 samples of T. cryptomerioides were determined. Eight different populations of T. cryptomerioides and 12 samples of T. flousiana from Yunnan, China were analyzed. The finding of the study showed that heterogeneity of ITS region within individuals of T. cryptomerioides was high by showing high nucleotide diversity among ITS sequences both in T. cryptomerioides ( £k = 0.18153) and T. flousiana ( £k = 0.19751). The findings fit in Tajima¡¦s D test of neutrality based on DNA sequence variation in the ITS region of T. cryptomerioides and T. flousiana. It is not obvious to incorporate into different population through clustering analysis based on data of the ITS region of T. cryptomerioides and T. flousiana. However, slightly genetic differentiation between T. cryptomerioides and T. flousiana was found, which figured of Fst (Fst = 0.0441~ 0.0856, an average value = 0.0611). On the other hand, the samples were studied by using ISSR markers. Of the 100 primers screened, 4 produced highly reproducible ISSR bands, and 24 discernible DNA fragments were generated with 17 being polymorphic. Based on cluster analysis of molecular data, the cluster is not clear among populations of T. cryptomerioides and T. flousiana. The analysis of AMOVA revealed that the variance component between species of T. cryptomerioides and T. flousiana was 38.54¢H (P < 0.001); however, the variance component within species is 61.46 (P < 0.001). The variation within population of T. cryptomerioides was 84.74¢H (P < 0.001) and the variance between populations is 15.26¢H (P < 0.001), indicating that the genetic diversity of individuals within population was high. The aforementioned data suggest that gene flow among different populations of T. cryptomerioides was high, indicating that the genetic diversity was high among individuals of T. cryptomerioides but was low between populations. Furthermore, it is concluded both species are genetically closer and could be grouped into the same species.

وصف الملف: application/pdf

الإتاحة: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0218105-011932Test