المؤلفون: Panda, Debadatta^{1, 1}, Kumar, M.², Mahalingam, L.³, Ravendran, M.⁴, Manickam, S.⁵, Senguttuvan, K.⁶

المصدر: Agricultural Science Digest - A Research Journal 43(2):176-180. 2023

المؤلفون: Meghana R. Sagar, Sushil Kumar, Dhramendra Patidar, Amar A. Sakure

المصدر: Journal of King Saud University: Science, Vol 34, Iss 8, Pp 102336- (2022)

مصطلحات موضوعية: ANOVA, Diploid cotton, SSR, ISSR, Fibre strength, Oil, Science (General), Q1-390

الوصف: Desi cotton (Gossypium herbaceum) is one of the important crops as it is valuable source of lint and spinnable fibre for textile industries. G. herbaceum is resistant to biotic and abiotic stresses and is sturdy crops species. The genetic diversity analysis of this crop is important for further improvement in its productivity to cap the gap between demand and supply of short staple cotton. Therefore, a set of 48 inbred lines of desi cotton were used in this study and their variability was estimated using morphological traits, yield parameters, seed physical properties, fibre quality parameters and seed chemical parameters. Similarly, 13 SSR and five ISSR markers were used for molecular diversity evaluation of germplasm. In this study, ANOVA showed significant differences among all genotypes for all the traits, demonstrating a substantial amount of genetic variability in studied genotypes. Morphological studies showed that genotype Radhanpur had higher seed yield (185.50 g/plant) and lint yield (68.60 g/plant). Physico-biochemical studies suggest that genotypes GVhv-845 had higher fibre strength (26.10 g/tex), Wagad (19.05%) was higher in oil and W8 (51.67%) had maximum seed protein. The Manhattan dissimilarity co-efficient based phenotypic diversity generated six main clusters. The average dissimilarity value among genotypes was 0.30, indicating moderate phenotypic variability. The dendrogram generated from pooled data of SSR and ISSR markers based on Jaccard’s similarity matrix grouped the genotypes into four main clusters. The genetic coefficient of similarity among the genotypes ranged from 0.15 to 0.70 with an average of 0.32. The present study revealed a low correlation between phenotypic and marker-based matrices(r = 0.09). Along with, low correlation, both the matrices placed a limited number of genotypes (nine) in the same clusters in their respective dendrograms. The low correlation indicated that the two methods were different and highly variable as molecular markers are neutral in behave than quantitative traits.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1018364722005171Test; https://doaj.org/toc/1018-3647Test

الوصول الحر: https://doaj.org/article/f45ebc2a5bf74653a2b2323c3c82fa53Test

المؤلفون: Naghera, Y. V.^{1, 1}, Vadodariya, K. V.¹, Patel, R. K.¹, Modha, K. G.¹, Vadodariya, G. D.¹, Vavdiya, P. A.¹, Mungra, K. S.¹, Patel, A. D.¹

المصدر: Electronic Journal of Plant Breeding 12(2):563-566. 2021

المؤلفون: Y. V. Naghera*, K. V. Vadodariya, R. K. Patel, K. G. Modha, G. D. Vadodariya, P. A. Vavdiya, K. S. Mungra and A. D. Patel

المصدر: Electronic Journal of Plant Breeding, Vol 12, Iss 2, Pp 563-566 (2021)

مصطلحات موضوعية: diploid cotton, additive, dominance and epistasis gene actions, Plant culture, SB1-1110

الوصف: Generation mean study was carried out in two interspecific crosses of diploid cotton i.e., GBhv-302 × PA-812 and GBhv-302 × ARBa-1502 using six parameter model. Simple scaling tests along with joint scaling test were carried out to check the sufficiency of additive-dominance model. The estimates of dominant (h) gene action were higher than the additive (d) gene action in one or more characters studied. Among epistasis, additive × additive (i) type of gene action was found to be significant in cross GBhv-302 × PA-812 for sympodia per plant and ginning outturn and in cross GBhv-302 × ARBa-1502 for sympodia per plant, bolls per plant and seed cotton yield per plant. While dominance × dominance (l) type of gene effect contributed significantly in cross GBhv-302 × PA-812 for sympodia per plant and in cross GBhv-302 × ARBa-1502 for sympodia per plant, average boll weight, bolls per plant, average boll weight and seed cotton yield per plant. Duplicate type of epistasis was observed for sympodia per plant in both the crosses, for bolls per plant and seed cotton yield per plant in cross GBhv-302 × ARBa-1502 and for ginning outturn in cross GBhv-302 × PA-812. While complementary type of gene interaction was detected for bolls per plant and seed cotton yield per plant in cross GBhv-302 × PA-812 and for average boll weight and ginning outturn in cross GBhv-302 × ARBa-1502.

وصف الملف: electronic resource

العلاقة: http://ejplantbreeding.org/index.php/EJPB/article/view/3773Test; https://doaj.org/toc/0975-928XTest

الوصول الحر: https://doaj.org/article/b10ad59d1c94494b8dd98919446dcb48Test

المؤلفون: Zhaoguo Li (1916818), Zhen Liu (74646), Yangyang Wei (436909), Yuling Liu (781309), Linxue Xing (11105633), Mengjie Liu (573303), Pengtao Li (584898), Quanwei Lu (781993), Renhai Peng (329085)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Evolutionary Biology, Ecology, Plant Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, diploid cotton genome, MIOX gene family, domesticated tetraploid cottons Gos., PCR, 12 MIOX genes, diploid cotton species, enzyme myo-inositol oxygenase, Numerous cis-acting elements, 6 MIOX genes, tetraploid cottons Gossypium tomentosum, 11 MIOX genes, MIOX genes, tetraploid cotton genome

الوصف: Multiple sequence alignments were conducted using ClustalW. (PDF)

العلاقة: https://figshare.com/articles/journal_contribution/Alignment_of_85_MIOX_protein_sequence_/14942288Test

الإتاحة: https://doi.org/10.1371/journal.pone.0254111.s001Test

المؤلفون: Zhaoguo Li (1916818), Zhen Liu (74646), Yangyang Wei (436909), Yuling Liu (781309), Linxue Xing (11105633), Mengjie Liu (573303), Pengtao Li (584898), Quanwei Lu (781993), Renhai Peng (329085)

مصطلحات موضوعية: Medicine, Microbiology, Genetics, Evolutionary Biology, Ecology, Plant Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, diploid cotton genome, MIOX gene family, domesticated tetraploid cottons Gos., PCR, 12 MIOX genes, diploid cotton species, enzyme myo-inositol oxygenase, Numerous cis-acting elements, 6 MIOX genes, tetraploid cottons Gossypium tomentosum, 11 MIOX genes, MIOX genes, tetraploid cotton genome

الوصف: The promoter sequences (2 kb upstream of ATG) of the MIOX genes were analysed by PlantCARE. (PDF)

العلاقة: https://figshare.com/articles/journal_contribution/Promoter_analyses_of_MIOX_genes_/14942291Test

الإتاحة: https://doi.org/10.1371/journal.pone.0254111.s002Test

المؤلفون: Atul S. Hande, Ishwarappa S. Katageri, Mangesh P. Jadhav, Sateesh Adiger, Savita Gamanagatti, Kethireddy Venkata Padmalatha, Gurusamy Dhandapani, Mogilicherla Kanakachari, Polumetla Ananda Kumar, Vanga Siva Reddy

المصدر: BMC Genomics, Vol 18, Iss 1, Pp 1-15 (2017)

مصطلحات موضوعية: Diploid cotton, Microarray, qRT PCR, Transcription factors, Biotechnology, TP248.13-248.65, Genetics, QH426-470

الوصف: Abstract Background Cotton fibre is a single cell and it is one of the best platforms for unraveling the genes express during various stages of fibre development. There are reports devoted to comparative transcriptome study on fiber cell initiation and elongation in tetraploid cultivated cotton. However, in the present investigation, comparative transcriptome study was made in diploid cultivated cotton using isogenic fuzzy-lintless (Fl) and normal fuzzy linted (FL) lines belong to Gossypium arboreum, diploid species at two stages, 0 and 10 dpa (days post anthesis), using Affymetrix cotton GeneChip genome array. Result Scanning electron microscopy (SEM) analysis uncovered the occurrence of few fibre cell initials in the Fl line as compared to many in Normal FL at −2 and 0 dpa. However, at 10 dpa there were no fibre cells found elongated in Fl but many elongated cells were found in FL line. Up-regulation of transcription factors, AP2-EREBP, C2H2, C3H, HB and WRKY was observed at 0 dpa whereas in 10 dpa transcription factors, AP2-EREBP, AUX/IAA, bHLH, C2H2, C3H, HB, MYB, NAC, Orphans, PLATZ and WRKY were found down regulated in Fl line. These transcription factors were mainly involved in metabolic pathways such as phytohormone signaling, energy metabolism of cell, fatty acid metabolism, secondary metabolism and other signaling pathways and are related directly or indirectly in fiber development. Quantitative real-time PCR was performed to check fold up or down-regulation of these genes and transcription factors (TFs) down regulated in mutants as compared to normal at 0 and 10 dpa. Conclusion This study elucidates that the up-regulation of transcription factors like AP2-EREBP, C2H2, C3H, HB, WRKY and phytohormone signaling genes at 0 dpa and their down-regulation at the 10 dpa might have constrain the fibre elongation in fuzzy-lintless line. Along with this the down-regulation of genes involved in synthesis of VLCFA chain, transcripts necessary for energy and cell wall metabolism, EXPANSINs, arabinogalactan proteins (AGPs), tubulin might also be the probable reason for reduced growth of fibres in the Fl. Plant receptor-like kinases (RLKs), Leucine Rich Repeats) LRR- family protein and signal transduction coding for mitogen-activated protein kinase (MAPK) cascade, have been engaged in coordination of cell elongation and SCW biosynthesis, down-regulation of these might loss the function leads to reduced fibre growth.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12864-017-4066-yTest; https://doaj.org/toc/1471-2164Test

الوصول الحر: https://doaj.org/article/e186897e9a5d43be9fb5af508dd55586Test

المؤلفون: Pirithiraj Uthirapathy, Murugan Marimuthu, Balasubramani Venkatasamy, Senguttuvan Kannan, N. Manikanda Boopathi, Hari Ramakrishnan Selladurai, Premalatha Nallathambi

المساهمون: Pirithiraj Uthirapathy, Murugan Marimuthu, Balasubramani Venkatasamy, Senguttuvan Kannan, N. Manikanda Boopathi, Hari Ramakrishnan Selladurai, Premalatha Nallathambi

المصدر: https://doi.org/10.1093/jee/toad136Test.

مصطلحات موضوعية: desi diploid cotton

جغرافية الموضوع: world

الوصف: Cotton-melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae), is emerging as a potential threat to cotton cultivation worldwide. The resistance categories in Gossypium arboreum to A. gossypii still need to be explored. We screened 87 G. arboreum and 20 Gossypium hirsutum genotypes against aphids under natural field conditions. Twenty-six selected genotypes from these 2 species were tested under glasshouse conditions for resistance categories (antixenosis, antibiosis, and tolerance). Resistance categories were assessed by no-choice antibiosis assay, free-choice aphid settling assay, cumulative aphid days using population buildup tests, chlorophyl loss index, and damage ratings. No-choice antibiosis experiment revealed that the G. arboreum genotypes GAM156, PA785, CNA1008, DSV1202, FDX235, AKA2009-6, DAS1032, DHH05-1, GAM532, and GAM216 had a significant adverse effect on aphid development time, longevity, and fecundity. Gossypium arboreum genotypes CISA111 and AKA2008-7 expressed a low level of antixenosis but possessed antibiosis and tolerance. Aphid resistance persisted uniformly at different plant developmental stages studied. The chlorophyl loss percentage and damage rating scores were lower in G. arboreum than in G. hirsutum genotypes, indicating the existence of tolerance in G. arboreum to aphids. Logical relations analysis of resistance contributing factors depicted the presence of antixenosis, antibiosis, and tolerance in the G. arboreum genotypes PA785, CNA1008, DSV1202, and FDX235, indicating their utility for evaluating the mechanisms of resistance and aphid resistance introgression breeding into G. hirsutum to develop commercially cultivated cotton lines. Graphical Abstract

وصف الملف: text/HTML

الإتاحة: https://doi.org/10.1093/jee/toad136Test

المؤلفون: Bipinchandra B. Kalbande, Anita S. Patil

المصدر: Journal of Genetic Engineering and Biotechnology, Vol 14, Iss 1, Pp 9-18 (2016)

مصطلحات موضوعية: In-planta transformation, Diploid cotton, Gossypium hirsutum cv LRK-516 Anjali, pBinAR, At-NPR1 gene, Cotyledonary leaves, Meristem cells, Transformation frequency, Biotechnology, TP248.13-248.65, Genetics, QH426-470

الوصف: A new method of transgenic development called “In-planta” transformation method, where Agrobacterium is used to infect the plantlets but the steps of in vitro regeneration of plants is totally avoided. In this study, we have reported a simple In-planta method for efficient transformation of diploid cotton Gossypium hirsutum cv LRK-516 Anjali using Agrobacterium tumefaciens EHA-105 harbouring recombinant binary vector plasmid pBinAR with Arabidopsis At-NPR1 gene. Four day old plantlets were used for transformation. A vertical cut was made at the junction of cotyledonary leaves, moderately bisecting the shoot tip and exposing meristem cells at apical meristem. This site was infected with Agrobacterium inoculum. The transgenic events obtained were tested positive for the presence of At-NPR1 gene with promoter nptII gene. They are also tested negative for vector backbone integration and Agrobacterium contamination in T0 events. With this method a transformation frequency of 6.89% was reported for the cv LRK-516.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1687157X16300105Test; https://doaj.org/toc/1687-157XTest

الوصول الحر: https://doaj.org/article/2eff9007f3ae467bb7f95c432e8c5de8Test

المؤلفون: Patil, Bhuvaneshwaragouda R., Deshpande, S. K., Patil, Malagouda, Jyotiba, S. S., Khadi, B. M.

المصدر: Crop Research 44(3):437-446. 2012