المؤلفون: Ries, Laure Nicolas Annick, Steenwyk, Jacob L., de Castro, Patrícia Alves, de Lima, Pollyne Borborema Almeida, Almeida, Fausto, de Assis, Leandro José, Manfiolli, Adriana Oliveira, Takahashi-Nakaguchi, Azusa, Kusuya, Yoko, Hagiwara, Daisuke, Takahashi, Hiroki, Wang, Xi, Obar, Joshua J., Rokas, Antonis, Goldman, Gustavo H.

المساهمون: Fundação Ãmaznia Paraense de Amparo à Pesquisa

المصدر: Frontiers in Microbiology ; volume 10 ; ISSN 1664-302X

الإتاحة: https://doi.org/10.3389/fmicb.2019.00854Test

المؤلفون: dos Reis, Thaila Fernanda, Nitsche, Benjamin M., de Lima, Pollyne Borborema Almeida, de Assis, Leandro José, Mellado, Laura, Harris, Steven D., Meyer, Vera, dos Santos, Renato A. Corrêa, Riaño-Pachón, Diego M., Ries, Laure Nicolas Annick, Goldman, Gustavo H.

مصطلحات موضوعية: 570 Biowissenschaften, Biologie, Aspergillus nidulans, glucose, HxtB, glucose signalling, glucose transporter

الوصف: One of the drawbacks during second-generation biofuel production from plant lignocellulosic biomass is the accumulation of glucose, the preferred carbon source of microorganisms, which causes the repression of hydrolytic enzyme secretion by industrially relevant filamentous fungi. Glucose sensing, subsequent transport and cellular signalling pathways have been barely elucidated in these organisms. This study therefore characterized the transcriptional response of the filamentous fungus Aspergillus nidulans to the presence of high and low glucose concentrations under continuous chemostat cultivation with the aim to identify novel factors involved in glucose sensing and signalling. Several transcription factor- and transporter-encoding genes were identified as being differentially regulated, including the previously characterized glucose and xylose transporter HxtB. HxtB was confirmed to be a low affinity glucose transporter, localizing to the plasma membrane under low- and high-glucose conditions. Furthermore, HxtB was shown to be involved in conidiation-related processes and may play a role in downstream glucose signalling. A gene predicted to encode the protein kinase PskA was also identified as being important for glucose metabolism. This study identified several proteins with predicted roles in glucose metabolic processes and provides a foundation for further investigation into the response of biotechnologically important filamentous fungi to glucose.

وصف الملف: application/pdf

العلاقة: https://depositonce.tu-berlinTest.de/handle/11303/7739; http://dx.doi.org/10.14279/depositonce-6917Test

الإتاحة: https://doi.org/10.14279/depositonce-6917Test
https://depositonce.tu-berlinTest.de/handle/11303/7739

المؤلفون: dos Reis, Thaila Fernanda, Silva, Lilian Pereira, de Castro, Patrícia Alves, Almeida de Lima, Pollyne Borborema, do Carmo, Rafaela Andrade, Marini, Marjorie Mendes, da Silveira, José Franco, Ferreira, Beatriz Henriques, Rodrigues, Fernando, Malavazi, Iran, Goldman, Gustavo H

المصدر: G3 Genes|Genomes|Genetics ; volume 8, issue 1, page 265-278 ; ISSN 2160-1836

الوصف: Genetic stability is extremely important for the survival of every living organism, and a very complex set of genes has evolved to cope with DNA repair upon DNA damage. Here, we investigated the Aspergillus fumigatus AtmA (Ataxia-telangiectasia mutated, ATM) and AtrA kinases, and how they impact virulence and the evolution of azole resistance. We demonstrated that A. fumigatus atmA and atrA null mutants are haploid and have a discrete chromosomal polymorphism. The ΔatmA and ΔatrA strains are sensitive to several DNA-damaging agents, but surprisingly both strains were more resistant than the wild-type strain to paraquat, menadione, and hydrogen peroxide. The atmA and atrA genes showed synthetic lethality emphasizing the cooperation between both enzymes and their consequent redundancy. The lack of atmA and atrA does not cause any significant virulence reduction in A. fumigatus in a neutropenic murine model of invasive pulmonary aspergillosis and in the invertebrate alternative model Galleria mellonela. Wild-type, ΔatmA, and ΔatrA populations that were previously transferred 10 times in minimal medium (MM) in the absence of voriconazole have not shown any significant changes in drug resistance acquisition. In contrast, ΔatmA and ΔatrA populations that similarly evolved in the presence of a subinhibitory concentration of voriconazole showed an ∼5–10-fold increase when compared to the original minimal inhibitory concentration (MIC) values. There are discrete alterations in the voriconazole target Cyp51A/Erg11A or cyp51/erg11 and/or Cdr1B efflux transporter overexpression that do not seem to be the main mechanisms to explain voriconazole resistance in these evolved populations. Taken together, these results suggest that genetic instability caused by ΔatmA and ΔatrA mutations can confer an adaptive advantage, mainly in the intensity of voriconazole resistance acquisition.

الإتاحة: https://doi.org/10.1534/g3.117.300265Test
http://academic.oup.com/g3journal/article-pdf/8/1/265/37183467/g3journal0265.pdfTest

المؤلفون: dos Reis, Thaila Fernanda, Nitsche, Benjamin M., de Lima, Pollyne Borborema Almeida, de Assis, Leandro José, Mellado, Laura, Harris, Steven D., Meyer, Vera, dos Santos, Renato A. Corrêa, Riaño-Pachón, Diego M., Ries, Laure Nicolas Annick, Goldman, Gustavo H.

المصدر: Scientific Reports ; volume 7, issue 1 ; ISSN 2045-2322

مصطلحات موضوعية: Multidisciplinary

الوصف: One of the drawbacks during second-generation biofuel production from plant lignocellulosic biomass is the accumulation of glucose, the preferred carbon source of microorganisms, which causes the repression of hydrolytic enzyme secretion by industrially relevant filamentous fungi. Glucose sensing, subsequent transport and cellular signalling pathways have been barely elucidated in these organisms. This study therefore characterized the transcriptional response of the filamentous fungus Aspergillus nidulans to the presence of high and low glucose concentrations under continuous chemostat cultivation with the aim to identify novel factors involved in glucose sensing and signalling. Several transcription factor- and transporter-encoding genes were identified as being differentially regulated, including the previously characterized glucose and xylose transporter HxtB. HxtB was confirmed to be a low affinity glucose transporter, localizing to the plasma membrane under low- and high-glucose conditions. Furthermore, HxtB was shown to be involved in conidiation-related processes and may play a role in downstream glucose signalling. A gene predicted to encode the protein kinase PskA was also identified as being important for glucose metabolism. This study identified several proteins with predicted roles in glucose metabolic processes and provides a foundation for further investigation into the response of biotechnologically important filamentous fungi to glucose.

الإتاحة: https://doi.org/10.1038/srep45073Test
https://www.nature.com/articles/srep45073.pdfTest
https://www.nature.com/articles/srep45073Test

المؤلفون: dos Reis, Thaila Fernanda, de Lima, Pollyne Borborema Almeida, Parachin, Nádia Skorupa, Mingossi, Fabiana Bombonato, de Castro Oliveira, Juliana Velasco, Ries, Laure Nicolas Annick, Goldman, Gustavo Henrique

المساهمون: Fundação de Amparo à Pesquisa do Estado de São Paulo, Conselho Nacional de Desenvolvimento Científico e Tecnológico

المصدر: Biotechnology for Biofuels ; volume 9, issue 1 ; ISSN 1754-6834

مصطلحات موضوعية: Management, Monitoring, Policy and Law, General Energy, Renewable Energy, Sustainability and the Environment, Applied Microbiology and Biotechnology, Biotechnology

الإتاحة: https://doi.org/10.1186/s13068-016-0611-1Test

المؤلفون: de Lima, Pollyne Borborema Almeida, Mulder, Kelly Cristina Leite, Melo, Nadiele Tamires Moreira, Carvalho, Lucas Silva, Menino, Gisele Soares, Mulinari, Eduardo, de Castro, Virgilio H., dos Reis, Thaila F., Goldman, Gustavo Henrique, Magalhães, Beatriz Simas, Parachin, Nádia Skorupa

المساهمون: Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação de Apoio à Pesquisa do Distrito Federal

المصدر: Microbial Cell Factories ; volume 15, issue 1 ; ISSN 1475-2859

مصطلحات موضوعية: Applied Microbiology and Biotechnology, Bioengineering, Biotechnology

الإتاحة: https://doi.org/10.1186/s12934-016-0557-9Test

المؤلفون: dos Reis, Thaila, de Lima, Pollyne, Parachin, Nádia, Mingossi, Fabiana, de Castro Oliveira, Juliana, Ries, Laure, Goldman, Gustavo

مصطلحات موضوعية: Aspergillus nidulans, Saccharomyces cerevisiae, Xylose, Cellobiose, Sugar transport

الوصف: Background The conversion of lignocellulosic biomass to biofuels (second-generation biofuel production) is an environmentally friendlier alternative to petroleum-based energy sources. Enzymatic deconstruction of lignocellulose, catalyzed by filamentous fungi such as Aspergillus nidulans , releases a mixture of mono- and polysaccharides, including hexose (glucose) and pentose (xylose) sugars, cellodextrins (cellobiose), and xylooligosaccharides (xylobiose). These sugars can subsequently be fermented by yeast cells to ethanol. One of the major drawbacks in this process lies in the inability of yeast, such as Saccharomyces cerevisiae , to successfully internalize sugars other than glucose. The aim of this study was, therefore, to screen the genome of A. nidulans , which encodes a multitude of sugar transporters, for transporters able to internalize non-glucose sugars and characterize them when introduced into S. cerevisiae . Results This work identified two proteins in A. nidulans , CltA and CltB, with roles in cellobiose transport and cellulose signaling, respectively. CltA, when introduced into S. cerevisiae , conferred growth on low and high concentrations of cellobiose. Deletion of cltB resulted in reduced growth and extracellular cellulase activity in A. nidulans in the presence of cellobiose. CltB, when introduced into S. cerevisiae , was not able to confer growth on cellobiose, suggesting that this protein is a sensor rather than a transporter. However, we have shown that the introduction of additional functional copies of CltB increases the growth in the presence of low concentrations of cellobiose, strongly indicating CltB is able to transport cellobiose. Furthermore, a previously identified glucose transporter, HxtB, was also found to be a major xylose transporter in A. nidulans . In S. cerevisiae , HxtB conferred growth on xylose which was accompanied by ethanol production. Conclusions This work identified a cellobiose transporter, a xylose transporter, and a putative cellulose transceptor in A. ...

العلاقة: http://www.biotechnologyforbiofuels.com/content/9/1/204Test

الإتاحة: http://www.biotechnologyforbiofuels.com/content/9/1/204Test

المؤلفون: de Lima, Pollyne, Mulder, Kelly, Melo, Nadiele, Carvalho, Lucas, Menino, Gisele, Mulinari, Eduardo, de Castro, Virgilio, dos Reis, Thaila, Goldman, Gustavo, Magalhães, Beatriz, Parachin, Nádia

مصطلحات موضوعية: l-Lactic acid, Pichia (Komagataella) pastoris, Lactate transporter, Oxygen limited fermentation, Lactate dehydrogenase

الوصف: Background Crude glycerol is the main byproduct of the biodiesel industry. Although it can have different applications, its purification is costly. Therefore, in this study a biotechnological route has been proposed for further utilization of crude glycerol in the fermentative production of lactic acid. This acid is largely utilized in food, pharmaceutical, textile, and chemical industries, making it the hydroxycarboxylic acid with the highest market potential worldwide. Currently, industrial production of lactic acid is done mainly using sugar as the substrate. Thus here, for the first time, Pichia pastoris has been engineered for heterologous l -lactic acid production using glycerol as a single carbon source. For that, the Bos taurus lactate dehydrogenase gene was introduced into P. pastoris . Moreover, a heterologous and a novel homologous lactate transporter have been evaluated for l -lactic acid production. Results Batch fermentation of the P. pastoris X-33 strain producing LDHb allowed for lactic acid production in this yeast. Although P. pastoris is known for its respiratory metabolism, batch fermentations were performed with different oxygenation levels, indicating that lower oxygen availability increased lactic acid production by 20 %, pushing the yeast towards a fermentative metabolism. Furthermore, a newly putative lactate transporter from P. pastoris named PAS has been identified by search similarity with the lactate transporter from Saccharomyces cerevisiae Jen1p. Both heterologous and homologous transporters, Jen1p and PAS, were evaluated in one strain already containing LDH activity. Fed-batch experiments of P. pastoris strains carrying the lactate transporter were performed with the batch phase at aerobic conditions followed by an aerobic oxygen-limited phase where production of lactic acid was favored. The results showed that the strain containing PAS presented the highest lactic acid titer, reaching a yield of approximately 0.7 g/g. Conclusions We showed that P. pastoris has a great ...

العلاقة: http://www.microbialcellfactories.com/content/15/1/158Test

الإتاحة: http://www.microbialcellfactories.com/content/15/1/158Test